##gff-version 3
##sequence-region BBa_K861177 1 1713
BBa_K861177	.	engineered_region	809	1713	.	+	0	ID=BBa_K861175;Name=BBa_K861175
BBa_K861177	.	promoter	809	844	.	+	0	ID=BBa_K861170;Name=BBa_K861170;Parent=BBa_K861175
BBa_K861177	.	non_covalent_binding_site	823	844	.	+	0	ID=annotation2183481;Name=Modified Consensus Crp binding site;Parent=BBa_K861170
BBa_K861177	.	engineered_region	853	1713	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_K861175
BBa_K861177	.	terminator	1673	1713	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K861177	.	stem_loop	1680	1699	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K861177	.	stop_codon	1706	1706	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K861177	.	polyA_site	1700	1713	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K861177	.	engineered_region	1673	1713	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K861177	.	ribosome_entry_site	853	864	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K861177	.	conserved_region	857	860	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K861177	.	terminator	1585	1664	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K861177	.	stem_loop	1596	1639	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K861177	.	engineered_region	1585	1664	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K861177	.	CDS	871	1551	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K861177	.	engineered_tag	1552	1576	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K861177	.	CDS	871	1545	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K861177	.	engineered_region	1	800	.	+	0	ID=BBa_K861162;Name=BBa_K861162
BBa_K861177	.	engineered_region	44	800	.	+	0	ID=BBa_K861161;Name=BBa_K861161;Parent=BBa_K861162
BBa_K861177	.	CDS	65	697	.	+	0	ID=BBa_K861160;Name=BBa_K861160;Parent=BBa_K861161
BBa_K861177	.	terminator	706	800	.	+	0	ID=BBa_B0024;Name=BBa_B0024;Parent=BBa_K861161
BBa_K861177	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K861161
BBa_K861177	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K861177	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K861177	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K861177	.	promoter	1	35	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K861162
>BBa_K861177
ttgacagctagctcagtcctagggactatgctagctactagagattaaagaggagaaatactagatggtgcttggcaaa
ccgcaaacagacccgactctcgaatggttcttgtctcattgccacattcataagtacccatccaagagcacgcttattc
accagggtgaaaaagcggaaacgctgtactacatcgttaaaggctctgtggcagtgctgatcaaagacgaagagggtaa
agaaatgatcctctcctatctgaatcagggtgattttattggcgaactgggcctgtttgaagagggccaggaacgtagc
gcatgggtacgtgcgaaaaccgcctgtgaagtggctgaaatttcgtacaaaaaatttcgccaattgattcaggtaaacc
cggacattctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgtt
cctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggt
atgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctgaaga
tgctggaagatcagaacctgatctccgcacacggtaaaaccatcgtcgtttacggcactcgttaatactagagaaataa
taaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtga
gccagtgtgatactagagttgacagctagctcaaatgtgattataatcacattttactagagaaagaggagaaatacta
gatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgag
ttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtc
cgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtt
accgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccg
acggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaa
aggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaa
aaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcg
ttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata