LOCUS       BBa_K861178             1713 bp    DNA     linear       17-May-2024
DEFINITION  Pcar efficacy testing device with CRP overexpressed
ACCESSION   BBa_K861178
VERSION     BBa_K861178.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23116
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             65..697
                     /label=BBa_K861160
     terminator      706..800
                     /label=BBa_B0024
     misc_feature    44..800
                     /label=BBa_K861161
     misc_feature    1..800
                     /label=BBa_K861162
     misc_feature    823..844
                     /label=Consensus CRP binding site
     promoter        809..844
                     /label=BBa_K861171
     misc_feature    857..860
                     /label=conserved
     RBS             853..864
                     /label=BBa_B0034
     CDS             871..1545
                     /label=mrfp1
     misc_feature    1552..1576
                     /label=Help:Barcodes
     CDS             871..1551
                     /label=BBa_E1010
     stem_loop       1596..1639
                     /label=stem_loop
     misc_feature    1585..1664
                     /label=BBa_B0010
     terminator      1585..1664
                     /label=BBa_B0010
     stem_loop       1680..1699
                     /label=T7 TE
     misc_feature    1706..1706
                     /label=stop
     polyA_site      1700..1713
                     /label=polya
     misc_feature    1673..1713
                     /label=BBa_B0012
     terminator      1673..1713
                     /label=BBa_B0012
     misc_feature    853..1713
                     /label=BBa_I13507
     misc_feature    809..1713
                     /label=BBa_K861176
ORIGIN
        1 ttgacagcta gctcagtcct agggactatg ctagctacta gagattaaag aggagaaata
       61 ctagatggtg cttggcaaac cgcaaacaga cccgactctc gaatggttct tgtctcattg
      121 ccacattcat aagtacccat ccaagagcac gcttattcac cagggtgaaa aagcggaaac
      181 gctgtactac atcgttaaag gctctgtggc agtgctgatc aaagacgaag agggtaaaga
      241 aatgatcctc tcctatctga atcagggtga ttttattggc gaactgggcc tgtttgaaga
      301 gggccaggaa cgtagcgcat gggtacgtgc gaaaaccgcc tgtgaagtgg ctgaaatttc
      361 gtacaaaaaa tttcgccaat tgattcaggt aaacccggac attctgatgc gtttgtctgc
      421 acagatggcg cgtcgtctgc aagtcacttc agagaaagtg ggcaacctgg cgttcctcga
      481 cgtgacgggc cgcattgcac agactctgct gaatctggca aaacaaccag acgctatgac
      541 tcacccggac ggtatgcaaa tcaaaattac ccgtcaggaa attggtcaga ttgtcggctg
      601 ttctcgtgaa accgtgggac gcattctgaa gatgctggaa gatcagaacc tgatctccgc
      661 acacggtaaa accatcgtcg tttacggcac tcgttaatac tagagaaata ataaaaaagc
      721 cggattaata atctggcttt ttatattctc tctctagtat ataaacgcag aaaggcccac
      781 ccgaaggtga gccagtgtga tactagagtt gacagctagc tcaaatgtga tttaaatcac
      841 attttactag agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag
      901 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa
      961 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa
     1021 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa
     1081 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt
     1141 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac
     1201 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg
     1261 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg
     1321 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt
     1381 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg
     1441 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
     1501 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag
     1561 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1621 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1681 gctcaccttc gggtgggcct ttctgcgttt ata
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