BBa_K112121
1
BBa_K112121
BglII endonuclease with ribosome binding site
2008-10-29T12:00:00Z
2015-05-08T01:09:14Z
<pre>
PCR mv032/mv010 on pBca9145-Bca1147 (722bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112121
</pre>
BglII nuclease with ribosome binding site and stop codon
false
false
_224_
0
3023
9
It's complicated
true
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.
---
<pre>
PCR mv032/mv010 on pBca9145-Bca1147 (722bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112121
-----------------------------------------------
mv032 Forward Biobricking of rbs.r.BglII!
cgataGAATTCatgAGATCTtggaaggagacactcataatgaagattgatataacggactataaccatgc
mv010 Reverse Biobricking of r.BglII!
cgttaGGATCCttaatatgtcacgattgttcctc
</pre>
false
Madhvi Venkatesh
BBa_K861201
1
BBa_K861201
Death Device designed for probiotics
2012-09-29T11:00:00Z
2015-05-08T01:13:36Z
None
We plan to place endonuclease BglII and YhjH gene downstream the xylR repressed promoter BBa_K861200 to make bacteria die without lysis and lose their adhesion when exposed to xylose.
false
false
_1121_
0
11007
9
Not in stock
false
None
false
Kuanwei Sheng
component2204307
1
BBa_K112121
component2204313
1
BBa_K861091
component2204306
1
BBa_K861200
annotation2204306
1
BBa_K861200
range2204306
1
1
49
annotation2204307
1
BBa_K112121
range2204307
1
58
753
annotation2204313
1
BBa_K861091
range2204313
1
762
1653
BBa_K861200
1
BBa_K861200
XylR repressed promoter
2012-09-29T11:00:00Z
2015-05-08T01:13:36Z
None
This synthetic promoter is aimed at being repressed by xylR from Bacillus subtilis and being derepressed by xylose. XylR binding seqence is overlapped with the -10 region of modified constitutive promoter. It is a part of our death device.
false
false
_1121_
0
11007
9
Not in stock
false
None
false
Kuanwei Sheng
annotation2204304
1
xylR binding site
range2204304
1
13
49
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_K861091
1
BBa_K861091
YhjH gene with a RBS and a Terminator
2012-09-17T11:00:00Z
2015-05-08T01:13:35Z
E.coli DH5α
YhjH gene can low the concentration of c-di-GMP in bacteria. Therefore make bacteria more motile. This part is the coding region of YhjH gene with a RBS and a terminator.
false
false
_1121_
0
11007
9
It's complicated
false
None
false
Kuanwei Sheng
component2185190
1
BBa_B0030
component2185193
1
BBa_B0024
component2185192
1
BBa_K861090
annotation2185192
1
BBa_K861090
range2185192
1
22
789
annotation2185193
1
BBa_B0024
range2185193
1
798
892
annotation2185190
1
BBa_B0030
range2185190
1
1
15
BBa_K861090
1
BBa_K861090
YhjH Gene From <i>E.coli str. K12</i>
2012-09-17T11:00:00Z
2015-05-08T01:13:35Z
E.coli DH5α
Released HQ 2013
YhjH protein contain a EAL domain to catalyze c-di-GMP into GMP. c-di-GMP is a magic molecule that can activates bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. Thus, the expression of YhjH gene can make the bacteria more motile.
false
true
_1121_
0
11007
9
In stock
false
None
false
Kuanwei Sheng
BBa_B0024
1
BBa_B0024
double terminator (B0012-B0011), reversed
2003-12-02T12:00:00Z
2015-08-31T04:07:20Z
-- No description --
false
false
_1_
0
24
7
In stock
false
true
Caitlin Conboy
BBa_B0024_sequence
1
aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
BBa_K861201_sequence
1
ttgacagctagcaaagcttttagtttgtttgggcaacaaactaaggtactactagaggatcttggaaggagacactcataatgaagattgatataacggactataaccatgctgatgaaatactaaatcctcaattatggaaagaaattgaagaaacgctattaaaaatgccattacacgttaaagcatcagatcaagccagcaaagttggcagtttaatttttgatcctgtcggtacaaatcaatacataaaagatgagttggtaccaaaacattggaaaaataatatccctatacctaaacgatttgacttcctaggtactgacatagattttggtaaaagagatacgctagttgaagttcagttttctaattacccatttctgctcaataatacggtacgttcagaactgtttcataaaagtaacatggacattgatgaagaaggaatgaaagtagcgatcattattactaaagggcatatgtttcccgcttctaacagttcattatattatgaacaagctcaaaatcaacttaactccttagctgaatataacgtttttgatgtacctataagattagtagggttaatagaagactttgaaactgatattgatattgtttcaactacatatgcggacaaacgctattcaaggacaataacaaaaagagataccgttaaaggtaaagtgattgataccaacacgccgaataccagacgtcggaaaagaggaacaatcgtgacatattaagtactagagattaaagaggagaaatactagatgataaggcaggttatccagcgaataagcaaccctgaagcaagcatcgagagcttgcaggaacggcgtttttggttgcagtgtgagcgtgcttacacctggcagccgatctatcaaacatgcgggcggttaatggccgtggagctattaacggtggtcacgcatcccttgaacccttcgcaacgcctgccgccggatcgctattttactgaaatcaccgtcagccatcggatggaggttgtgaaagagcagattgatttgctggcgcaaaaagccgacttctttatagagcacggcctgctggcatcggtcaatattgatggccctacgctcatcgccctgcgtcagcaaccaaaaatcctgcgccagattgagcgtcttccctggctgcgtttcgaactggtggagcatatccgtctgccgaaagattcaacctttgcctcgatgtgtgaatttggcccgctgtggctggatgattttggtaccgggatggcaaatttctctgcgctaagtgaagtgcgttatgactacatcaaaatcgcgcgagaactgtttgtgatgctgcgtcagtcgccggaaggacgcacactcttttctcagcttttacatctaatgaatcgctattgtcgcggggtgattgtcgagggcgtagaaacgccggaagagtggcgtgatgttcagaactcgcccgcattcgccgcacaaggctggtttctttcacgcccggcaccgatagaaacgctgaatacggcggttctggcgctataatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K112121_sequence
1
gatcttggaaggagacactcataatgaagattgatataacggactataaccatgctgatgaaatactaaatcctcaattatggaaagaaattgaagaaacgctattaaaaatgccattacacgttaaagcatcagatcaagccagcaaagttggcagtttaatttttgatcctgtcggtacaaatcaatacataaaagatgagttggtaccaaaacattggaaaaataatatccctatacctaaacgatttgacttcctaggtactgacatagattttggtaaaagagatacgctagttgaagttcagttttctaattacccatttctgctcaataatacggtacgttcagaactgtttcataaaagtaacatggacattgatgaagaaggaatgaaagtagcgatcattattactaaagggcatatgtttcccgcttctaacagttcattatattatgaacaagctcaaaatcaacttaactccttagctgaatataacgtttttgatgtacctataagattagtagggttaatagaagactttgaaactgatattgatattgtttcaactacatatgcggacaaacgctattcaaggacaataacaaaaagagataccgttaaaggtaaagtgattgataccaacacgccgaataccagacgtcggaaaagaggaacaatcgtgacatattaag
BBa_K861200_sequence
1
ttgacagctagcaaagcttttagtttgtttgggcaacaaactaaggtac
BBa_K861090_sequence
1
atgataaggcaggttatccagcgaataagcaaccctgaagcaagcatcgagagcttgcaggaacggcgtttttggttgcagtgtgagcgtgcttacacctggcagccgatctatcaaacatgcgggcggttaatggccgtggagctattaacggtggtcacgcatcccttgaacccttcgcaacgcctgccgccggatcgctattttactgaaatcaccgtcagccatcggatggaggttgtgaaagagcagattgatttgctggcgcaaaaagccgacttctttatagagcacggcctgctggcatcggtcaatattgatggccctacgctcatcgccctgcgtcagcaaccaaaaatcctgcgccagattgagcgtcttccctggctgcgtttcgaactggtggagcatatccgtctgccgaaagattcaacctttgcctcgatgtgtgaatttggcccgctgtggctggatgattttggtaccgggatggcaaatttctctgcgctaagtgaagtgcgttatgactacatcaaaatcgcgcgagaactgtttgtgatgctgcgtcagtcgccggaaggacgcacactcttttctcagcttttacatctaatgaatcgctattgtcgcggggtgattgtcgagggcgtagaaacgccggaagagtggcgtgatgttcagaactcgcccgcattcgccgcacaaggctggtttctttcacgcccggcaccgatagaaacgctgaatacggcggttctggcgctataa
BBa_K861091_sequence
1
attaaagaggagaaatactagatgataaggcaggttatccagcgaataagcaaccctgaagcaagcatcgagagcttgcaggaacggcgtttttggttgcagtgtgagcgtgcttacacctggcagccgatctatcaaacatgcgggcggttaatggccgtggagctattaacggtggtcacgcatcccttgaacccttcgcaacgcctgccgccggatcgctattttactgaaatcaccgtcagccatcggatggaggttgtgaaagagcagattgatttgctggcgcaaaaagccgacttctttatagagcacggcctgctggcatcggtcaatattgatggccctacgctcatcgccctgcgtcagcaaccaaaaatcctgcgccagattgagcgtcttccctggctgcgtttcgaactggtggagcatatccgtctgccgaaagattcaacctttgcctcgatgtgtgaatttggcccgctgtggctggatgattttggtaccgggatggcaaatttctctgcgctaagtgaagtgcgttatgactacatcaaaatcgcgcgagaactgtttgtgatgctgcgtcagtcgccggaaggacgcacactcttttctcagcttttacatctaatgaatcgctattgtcgcggggtgattgtcgagggcgtagaaacgccggaagagtggcgtgatgttcagaactcgcccgcattcgccgcacaaggctggtttctttcacgcccggcaccgatagaaacgctgaatacggcggttctggcgctataatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z