BBa_K112121 1 BBa_K112121 BglII endonuclease with ribosome binding site 2008-10-29T12:00:00Z 2015-05-08T01:09:14Z <pre> PCR mv032/mv010 on pBca9145-Bca1147 (722bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112121 </pre> BglII nuclease with ribosome binding site and stop codon false false _224_ 0 3023 9 It's complicated true This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. --- <pre> PCR mv032/mv010 on pBca9145-Bca1147 (722bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112121 ----------------------------------------------- mv032 Forward Biobricking of rbs.r.BglII! cgataGAATTCatgAGATCTtggaaggagacactcataatgaagattgatataacggactataaccatgc mv010 Reverse Biobricking of r.BglII! cgttaGGATCCttaatatgtcacgattgttcctc </pre> false Madhvi Venkatesh BBa_K861201 1 BBa_K861201 Death Device designed for probiotics 2012-09-29T11:00:00Z 2015-05-08T01:13:36Z None We plan to place endonuclease BglII and YhjH gene downstream the xylR repressed promoter BBa_K861200 to make bacteria die without lysis and lose their adhesion when exposed to xylose. false false _1121_ 0 11007 9 Not in stock false None false Kuanwei Sheng component2204307 1 BBa_K112121 component2204313 1 BBa_K861091 component2204306 1 BBa_K861200 annotation2204306 1 BBa_K861200 range2204306 1 1 49 annotation2204307 1 BBa_K112121 range2204307 1 58 753 annotation2204313 1 BBa_K861091 range2204313 1 762 1653 BBa_K861200 1 BBa_K861200 XylR repressed promoter 2012-09-29T11:00:00Z 2015-05-08T01:13:36Z None This synthetic promoter is aimed at being repressed by xylR from Bacillus subtilis and being derepressed by xylose. XylR binding seqence is overlapped with the -10 region of modified constitutive promoter. It is a part of our death device. false false _1121_ 0 11007 9 Not in stock false None false Kuanwei Sheng annotation2204304 1 xylR binding site range2204304 1 13 49 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 BBa_K861091 1 BBa_K861091 YhjH gene with a RBS and a Terminator 2012-09-17T11:00:00Z 2015-05-08T01:13:35Z E.coli DH5&#945; YhjH gene can low the concentration of c-di-GMP in bacteria. Therefore make bacteria more motile. This part is the coding region of YhjH gene with a RBS and a terminator. false false _1121_ 0 11007 9 It's complicated false None false Kuanwei Sheng component2185190 1 BBa_B0030 component2185193 1 BBa_B0024 component2185192 1 BBa_K861090 annotation2185192 1 BBa_K861090 range2185192 1 22 789 annotation2185193 1 BBa_B0024 range2185193 1 798 892 annotation2185190 1 BBa_B0030 range2185190 1 1 15 BBa_K861090 1 BBa_K861090 YhjH Gene From <i>E.coli str. K12</i> 2012-09-17T11:00:00Z 2015-05-08T01:13:35Z E.coli DH5&#945; Released HQ 2013 YhjH protein contain a EAL domain to catalyze c-di-GMP into GMP. c-di-GMP is a magic molecule that can activates bcsB gene, a major component of cellulose synthetase complex. Also, c-di-GMP can serve as a second messager to inhibit motility and increse biofilm formation and adhesion of bacteria. Thus, the expression of YhjH gene can make the bacteria more motile. false true _1121_ 0 11007 9 In stock false None false Kuanwei Sheng BBa_B0024 1 BBa_B0024 double terminator (B0012-B0011), reversed 2003-12-02T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy BBa_B0024_sequence 1 aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_K861201_sequence 1 ttgacagctagcaaagcttttagtttgtttgggcaacaaactaaggtactactagaggatcttggaaggagacactcataatgaagattgatataacggactataaccatgctgatgaaatactaaatcctcaattatggaaagaaattgaagaaacgctattaaaaatgccattacacgttaaagcatcagatcaagccagcaaagttggcagtttaatttttgatcctgtcggtacaaatcaatacataaaagatgagttggtaccaaaacattggaaaaataatatccctatacctaaacgatttgacttcctaggtactgacatagattttggtaaaagagatacgctagttgaagttcagttttctaattacccatttctgctcaataatacggtacgttcagaactgtttcataaaagtaacatggacattgatgaagaaggaatgaaagtagcgatcattattactaaagggcatatgtttcccgcttctaacagttcattatattatgaacaagctcaaaatcaacttaactccttagctgaatataacgtttttgatgtacctataagattagtagggttaatagaagactttgaaactgatattgatattgtttcaactacatatgcggacaaacgctattcaaggacaataacaaaaagagataccgttaaaggtaaagtgattgataccaacacgccgaataccagacgtcggaaaagaggaacaatcgtgacatattaagtactagagattaaagaggagaaatactagatgataaggcaggttatccagcgaataagcaaccctgaagcaagcatcgagagcttgcaggaacggcgtttttggttgcagtgtgagcgtgcttacacctggcagccgatctatcaaacatgcgggcggttaatggccgtggagctattaacggtggtcacgcatcccttgaacccttcgcaacgcctgccgccggatcgctattttactgaaatcaccgtcagccatcggatggaggttgtgaaagagcagattgatttgctggcgcaaaaagccgacttctttatagagcacggcctgctggcatcggtcaatattgatggccctacgctcatcgccctgcgtcagcaaccaaaaatcctgcgccagattgagcgtcttccctggctgcgtttcgaactggtggagcatatccgtctgccgaaagattcaacctttgcctcgatgtgtgaatttggcccgctgtggctggatgattttggtaccgggatggcaaatttctctgcgctaagtgaagtgcgttatgactacatcaaaatcgcgcgagaactgtttgtgatgctgcgtcagtcgccggaaggacgcacactcttttctcagcttttacatctaatgaatcgctattgtcgcggggtgattgtcgagggcgtagaaacgccggaagagtggcgtgatgttcagaactcgcccgcattcgccgcacaaggctggtttctttcacgcccggcaccgatagaaacgctgaatacggcggttctggcgctataatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_B0030_sequence 1 attaaagaggagaaa BBa_K112121_sequence 1 gatcttggaaggagacactcataatgaagattgatataacggactataaccatgctgatgaaatactaaatcctcaattatggaaagaaattgaagaaacgctattaaaaatgccattacacgttaaagcatcagatcaagccagcaaagttggcagtttaatttttgatcctgtcggtacaaatcaatacataaaagatgagttggtaccaaaacattggaaaaataatatccctatacctaaacgatttgacttcctaggtactgacatagattttggtaaaagagatacgctagttgaagttcagttttctaattacccatttctgctcaataatacggtacgttcagaactgtttcataaaagtaacatggacattgatgaagaaggaatgaaagtagcgatcattattactaaagggcatatgtttcccgcttctaacagttcattatattatgaacaagctcaaaatcaacttaactccttagctgaatataacgtttttgatgtacctataagattagtagggttaatagaagactttgaaactgatattgatattgtttcaactacatatgcggacaaacgctattcaaggacaataacaaaaagagataccgttaaaggtaaagtgattgataccaacacgccgaataccagacgtcggaaaagaggaacaatcgtgacatattaag BBa_K861200_sequence 1 ttgacagctagcaaagcttttagtttgtttgggcaacaaactaaggtac BBa_K861090_sequence 1 atgataaggcaggttatccagcgaataagcaaccctgaagcaagcatcgagagcttgcaggaacggcgtttttggttgcagtgtgagcgtgcttacacctggcagccgatctatcaaacatgcgggcggttaatggccgtggagctattaacggtggtcacgcatcccttgaacccttcgcaacgcctgccgccggatcgctattttactgaaatcaccgtcagccatcggatggaggttgtgaaagagcagattgatttgctggcgcaaaaagccgacttctttatagagcacggcctgctggcatcggtcaatattgatggccctacgctcatcgccctgcgtcagcaaccaaaaatcctgcgccagattgagcgtcttccctggctgcgtttcgaactggtggagcatatccgtctgccgaaagattcaacctttgcctcgatgtgtgaatttggcccgctgtggctggatgattttggtaccgggatggcaaatttctctgcgctaagtgaagtgcgttatgactacatcaaaatcgcgcgagaactgtttgtgatgctgcgtcagtcgccggaaggacgcacactcttttctcagcttttacatctaatgaatcgctattgtcgcggggtgattgtcgagggcgtagaaacgccggaagagtggcgtgatgttcagaactcgcccgcattcgccgcacaaggctggtttctttcacgcccggcaccgatagaaacgctgaatacggcggttctggcgctataa BBa_K861091_sequence 1 attaaagaggagaaatactagatgataaggcaggttatccagcgaataagcaaccctgaagcaagcatcgagagcttgcaggaacggcgtttttggttgcagtgtgagcgtgcttacacctggcagccgatctatcaaacatgcgggcggttaatggccgtggagctattaacggtggtcacgcatcccttgaacccttcgcaacgcctgccgccggatcgctattttactgaaatcaccgtcagccatcggatggaggttgtgaaagagcagattgatttgctggcgcaaaaagccgacttctttatagagcacggcctgctggcatcggtcaatattgatggccctacgctcatcgccctgcgtcagcaaccaaaaatcctgcgccagattgagcgtcttccctggctgcgtttcgaactggtggagcatatccgtctgccgaaagattcaacctttgcctcgatgtgtgaatttggcccgctgtggctggatgattttggtaccgggatggcaaatttctctgcgctaagtgaagtgcgttatgactacatcaaaatcgcgcgagaactgtttgtgatgctgcgtcagtcgccggaaggacgcacactcttttctcagcttttacatctaatgaatcgctattgtcgcggggtgattgtcgagggcgtagaaacgccggaagagtggcgtgatgttcagaactcgcccgcattcgccgcacaaggctggtttctttcacgcccggcaccgatagaaacgctgaatacggcggttctggcgctataatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z