LOCUS BBa_K861345 2779 bp DNA linear 27-Dec-2024 DEFINITION IPTG induced S-fadE ACCESSION BBa_K861345 VERSION BBa_K861345.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..78 /label=RBS-1\Strong RBS 71..75 /label=RBS misc_feature 64..78 /label=BBa_B0030 RBS 64..78 /label=BBa_B0030 CDS 85..2676 /label=BBa_K861021 terminator 2685..2779 /label=BBa_B0024 misc_feature 64..2779 /label=BBa_K861023 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagattaaag aggagaaata ctagatgatg attttgagta ttatcgctac ggttgttctg 121 ctcggcgcat tgttctatca ccgcgtgagt ttatttctca gcagcctgat tctgctcgcc 181 tggaccgccg cacttggcgt cgctggcctg tggtccatct ggctgttggt tatgatgatt 241 ttgagtatta tcgctacggt tgttctgctc ggcgcattgt tctatcaccg cgtgagttta 301 tttctcagca gcctgattct gctcgcctgg accgccgcac ttggcgtcgc tggcctgtgg 361 tccatctggc tgttggttcc tctggccatt atcctcgtgc cgtttaacct cacaccgatg 421 cgaaaatcaa tgatttccgc accggtgttc cgcggcttcc gtaaggtcat gccgccgatg 481 tcgcgcactg agaaagaagc gattgatgcc ggtacaacct ggtgggaagg cgatctgttc 541 cagggcaagc ctgactggaa aaaactgcac aactatccgc aaccgcaact cactgccgaa 601 gagcaagcgt ttctcgacgg tccggtagaa gaagcgtgcc ggatggcgaa cgatttccag 661 attacccatg aactggcgga tctgccgccg gaactgtggg cttatctgaa agagcatcgt 721 ttttttgcga tgatcattaa aaaagagtac ggcggactgg agttctccgc ttatgcgcag 781 tcccgtgtac tgcaaaaact gtccggcgtc tccgggattc tggctatcac cgtcggcgtt 841 ccaaactcac tcggccctgg cgagttgttg cagcactatg gtacggaaga gcagaagaat 901 cactatctgc cgcgactggc gcgcggccag gagatcccct gctttgcgct gaccagcccg 961 gaagcaggtt ctgatgcagg cgccattccg gataccggcg tggtttgcat gggcgaatgg 1021 cagggccagc aggtgctggg tatgcgcctg acctggaaca agcgttacat tacgctggca 1081 ccgatagcta ccgtgttggg cctggcgttt aagctttccg atccggatag attgttaggc 1141 ggagaagaag agctgggtat tacctgtgcc ctgatcccca cctcaacgcc gggtgtggaa 1201 attggtcgtc gtcacttccc gcttaacgtt ccgttccaga atggtccaac ccgcggtaac 1261 gatattttcg tgcctattga ttatattatc ggcggcccca aaatggccgg acaaggctgg 1321 cgtatgctgg tggaatgttt gtcggtagga cgcggtatta ccctgccgtc caactccact 1381 ggcggggtga aatccgtcgc gctggcaacc ggcgcctatg ctcacattcg tcgtcagttc 1441 aaaatatcta tcggcaagat ggaaggtatt gaggaaccgc tggcgcgtat tgcaggtaac 1501 gcttatgtga tggatgccgc cgcatcgtta atcacctatg gcattatgct gggtgaaaaa 1561 ccggcggtac tgtcggctat cgtgaagtac cactgcaccc accgtggcca gcagtccatt 1621 atcgacgcga tggatatcac tgggggtaaa ggcattatgc tgggcgagag caacttcctg 1681 gcgcgtgctt accagggcgc gcctatcgcg atcaccgtgg aaggcgcgaa tatcctgacc 1741 cgtagcatga tgatctttgg tcagggcgcc attcgctgcc atccttatgt actggaagag 1801 atggccgcag cgcagaataa tgatgtgaat gcctttgata agctgttgtt taaacatatc 1861 ggccatgtcg gcagcaatac cgtccgcagt ttctggcttg gcctgacccg cggattaacg 1921 agccatacgc caaccggcga cgccacgaag cgttattacc agcatctgaa ccgactgagc 1981 gcaaacctgg cgctgctgtc tgacgtgtca atggcggtac tgggcggcag cctgaaacgc 2041 cgcgagcgta tctccgcgcg tctgggcgat gtgttgagtc agctttatct ggcctcagcc 2101 gtactgaagc gttacgacga cgaaggtcgc cacgaagccg acctgccgct ggtgcactgg 2161 ggcgtacagg atgcgctgta tcgtgctgaa caagcgatgg acgatctgct gcaaaacttc 2221 ccgaaccgcg tcgtggccgg attgctcacc gcgatgatct tcccgactgg ccgtcattat 2281 ctggcgccgt ctgataagct ggatcacgcc gtggcgaaga tcctacaggt gccgaacgcc 2341 acacgttcac gcattgggcg cggtcagtat ctgacgcccg ccgaacataa tccggtcggt 2401 ttactggaag aagcgctgcg tgatgtcatc gctgccgacc ctatccacca gcgcatctgc 2461 aaagaactgg gtaaaaatct gccgtttact cgtctggatg aactggcgcg caatgcgctg 2521 gcgaaagggc ttatagataa agatgaagcc gccatcctgg cgaaagcgga agagagccgc 2581 ctgcgcagta tcaacgttga tgattttgaa cctgaagcgc tggcgaccaa accggtaaag 2641 ctgccggaga aagtgcgtaa agtcgaagcc gcataatact agagaaataa taaaaaagcc 2701 ggattaataa tctggctttt tatattctct ctctagtata taaacgcaga aaggcccacc 2761 cgaaggtgag ccagtgtga //