BBa_K861041 1 BBa_K861041 FadI, gene for fatty acid degradation from <i>E.coli str. K12</i> 2012-09-05T11:00:00Z 2015-05-08T01:13:35Z E.coli str.K12 lab mutant DH5&#945; FadI is the anaerobic counterpart of FadA. It is shown to have better degradation of medium and short length fatty acid together with FadJ false false _1121_ 0 12357 9 It's complicated false None false Kuanwei Sheng BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_K861350 1 BBa_K861350 PfadR with FadI, intermediate part for BBa_K861047 2012-09-20T11:00:00Z 2015-05-08T01:13:36Z E.coli K12 DH5&#945; PfadR with FadI, a intermediate part for BBa_K861047 false false _1121_ 0 11007 9 It's complicated false None false Kuanwei Sheng component2191336 1 BBa_K861044 component2191331 1 BBa_K861060 annotation2191331 1 BBa_K861060 range2191331 1 1 76 annotation2191336 1 BBa_K861044 range2191336 1 85 1416 BBa_K861044 1 BBa_K861044 FadI with a RBS 2012-09-05T11:00:00Z 2015-05-08T01:13:35Z E.coli str.K12 lab mutant DH5&#945; FadI is the anaerobic counterpart of FadA. It is shown to have better degradation of medium and short length fatty acid together with FadJ.This device contains FadI with a RBS and a terminator. false false _1121_ 0 12357 9 It's complicated false None false Kuanwei Sheng component2182626 1 BBa_B0030 component2182628 1 BBa_K861041 annotation2182626 1 BBa_B0030 range2182626 1 1 15 annotation2182628 1 BBa_K861041 range2182628 1 22 1332 BBa_K861060 1 PfadR PfadR, synthetic promoter with tandem FadR binding site 2012-09-07T11:00:00Z 2015-05-08T01:13:35Z Escherichia coli str. K-12 substr. MG1655 At least to our knowledge, there is no promoter exists in nature that can respond solely to FadR since those promoters are often regulated by glucose concentration or oxidative stress and many other factors. We decided to design an artificial promoter to fulfill this task. Specifically, a tandem FadR binding site in FadL promoter is place downstream of promoter J23110 with 3 base pair overlap. We test its function in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1. false false _1121_ 0 12357 9 In stock true None false Kuanwei Sheng annotation2183423 1 FadR tandem binding site range2183423 1 33 76 BBa_K861060_sequence 1 tttacggctagctcagtcctaggtacaatgctagctggtccgacctatactctcgccactggtctgatttctaaga BBa_K861041_sequence 1 atgggtcaggttttaccgctggttacccgccagggcgatcgtatcgccattgttagcggtttacgtacgccttttgcccgtcaggcgacggcttttcatggcattcccgcggttgatttagggaagatggtggtaggcgaactgctggcacgcagcgagatccccgccgaagtgattgaacaactggtctttggtcaggtcgtacaaatgcctgaagcccccaacattgcgcgtgaaattgttctcggtacgggaatgaatgtacataccgatgcttacagcgtcagccgcgcttgcgctaccagtttccaggcagttgcaaacgtcgcagaaagcctgatggcgggaactattcgagcggggattgccggtggggcagattcctcttcggtattgccaattggcgtcagtaaaaaactggcgcgcgtgctggttgatgtcaacaaagctcgtaccatgagccagcgactgaaactcttctctcgcctgcgtttgcgcgacttaatgcccgtaccacctgcggtagcagaatattctaccggcttgcggatgggcgacaccgcagagcaaatggcgaaaacctacggcatcacccgagaacagcaagatgcattagcgcaccgttcgcatcagcgtgccgctcaggcatggtcagacggaaaactcaaagaagaggtgatgactgcctttatccctccttataaacaaccgcttgtcgaagacaacaatattcgcggtaattcctcgcttgccgattacgcaaagctgcgcccggcgtttgatcgcaaacacggaacggtaacggcggcaaacagtacgccgctgaccgatggcgcggcagcggtgatcctgatgactgaatcccgggcgaaagaattagggctggtgccgctggggtatctgcgcagctacgcatttactgcgattgatgtctggcaggacatgttgctcggtccagcctggtcaacaccgctggcgctggagcgtgccggtttgacgatgagcgatctgacattgatcgatatgcacgaagcctttgcagctcagacgctggcgaatattcagttgctgggtagtgaacgttttgctcgtgaagcactggggcgtgcacatgccactggcgaagtggacgatagcaaatttaacgtgcttggcggttcgattgcttacgggcatcccttcgcggcgaccggcgcgcggatgattacccagacattgcatgaacttcgccgtcgcggcggtggatttggtttagttaccgcctgtgctgccggtgggcttggcgcggcaatggttctggaggcggaataa BBa_K861044_sequence 1 attaaagaggagaaatactagatgggtcaggttttaccgctggttacccgccagggcgatcgtatcgccattgttagcggtttacgtacgccttttgcccgtcaggcgacggcttttcatggcattcccgcggttgatttagggaagatggtggtaggcgaactgctggcacgcagcgagatccccgccgaagtgattgaacaactggtctttggtcaggtcgtacaaatgcctgaagcccccaacattgcgcgtgaaattgttctcggtacgggaatgaatgtacataccgatgcttacagcgtcagccgcgcttgcgctaccagtttccaggcagttgcaaacgtcgcagaaagcctgatggcgggaactattcgagcggggattgccggtggggcagattcctcttcggtattgccaattggcgtcagtaaaaaactggcgcgcgtgctggttgatgtcaacaaagctcgtaccatgagccagcgactgaaactcttctctcgcctgcgtttgcgcgacttaatgcccgtaccacctgcggtagcagaatattctaccggcttgcggatgggcgacaccgcagagcaaatggcgaaaacctacggcatcacccgagaacagcaagatgcattagcgcaccgttcgcatcagcgtgccgctcaggcatggtcagacggaaaactcaaagaagaggtgatgactgcctttatccctccttataaacaaccgcttgtcgaagacaacaatattcgcggtaattcctcgcttgccgattacgcaaagctgcgcccggcgtttgatcgcaaacacggaacggtaacggcggcaaacagtacgccgctgaccgatggcgcggcagcggtgatcctgatgactgaatcccgggcgaaagaattagggctggtgccgctggggtatctgcgcagctacgcatttactgcgattgatgtctggcaggacatgttgctcggtccagcctggtcaacaccgctggcgctggagcgtgccggtttgacgatgagcgatctgacattgatcgatatgcacgaagcctttgcagctcagacgctggcgaatattcagttgctgggtagtgaacgttttgctcgtgaagcactggggcgtgcacatgccactggcgaagtggacgatagcaaatttaacgtgcttggcggttcgattgcttacgggcatcccttcgcggcgaccggcgcgcggatgattacccagacattgcatgaacttcgccgtcgcggcggtggatttggtttagttaccgcctgtgctgccggtgggcttggcgcggcaatggttctggaggcggaataa BBa_B0030_sequence 1 attaaagaggagaaa BBa_K861350_sequence 1 tttacggctagctcagtcctaggtacaatgctagctggtccgacctatactctcgccactggtctgatttctaagatactagagattaaagaggagaaatactagatgggtcaggttttaccgctggttacccgccagggcgatcgtatcgccattgttagcggtttacgtacgccttttgcccgtcaggcgacggcttttcatggcattcccgcggttgatttagggaagatggtggtaggcgaactgctggcacgcagcgagatccccgccgaagtgattgaacaactggtctttggtcaggtcgtacaaatgcctgaagcccccaacattgcgcgtgaaattgttctcggtacgggaatgaatgtacataccgatgcttacagcgtcagccgcgcttgcgctaccagtttccaggcagttgcaaacgtcgcagaaagcctgatggcgggaactattcgagcggggattgccggtggggcagattcctcttcggtattgccaattggcgtcagtaaaaaactggcgcgcgtgctggttgatgtcaacaaagctcgtaccatgagccagcgactgaaactcttctctcgcctgcgtttgcgcgacttaatgcccgtaccacctgcggtagcagaatattctaccggcttgcggatgggcgacaccgcagagcaaatggcgaaaacctacggcatcacccgagaacagcaagatgcattagcgcaccgttcgcatcagcgtgccgctcaggcatggtcagacggaaaactcaaagaagaggtgatgactgcctttatccctccttataaacaaccgcttgtcgaagacaacaatattcgcggtaattcctcgcttgccgattacgcaaagctgcgcccggcgtttgatcgcaaacacggaacggtaacggcggcaaacagtacgccgctgaccgatggcgcggcagcggtgatcctgatgactgaatcccgggcgaaagaattagggctggtgccgctggggtatctgcgcagctacgcatttactgcgattgatgtctggcaggacatgttgctcggtccagcctggtcaacaccgctggcgctggagcgtgccggtttgacgatgagcgatctgacattgatcgatatgcacgaagcctttgcagctcagacgctggcgaatattcagttgctgggtagtgaacgttttgctcgtgaagcactggggcgtgcacatgccactggcgaagtggacgatagcaaatttaacgtgcttggcggttcgattgcttacgggcatcccttcgcggcgaccggcgcgcggatgattacccagacattgcatgaacttcgccgtcgcggcggtggatttggtttagttaccgcctgtgctgccggtgggcttggcgcggcaatggttctggaggcggaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z