LOCUS BBa_K862001 3290 bp DNA linear 22-Nov-2024 DEFINITION psulA-LacZ-doubleTerminator ACCESSION BBa_K862001 VERSION BBa_K862001.1 FEATURES Location/Qualifiers promoter 1..52 /label=BBa_K518010 misc_feature 65..68 /label=conserved RBS 61..72 /label=BBa_B0034 misc_feature 3102..3102 /label=C->T CDS 79..3153 /label=lacZ misc_feature 79..81 /label=START misc_feature 3151..3153 /label=STOP CDS 79..3153 /label=BBa_I732005 stem_loop 3173..3216 /label=stem_loop misc_feature 3162..3241 /label=BBa_B0010 terminator 3162..3241 /label=BBa_B0010 stem_loop 3257..3276 /label=T7 TE misc_feature 3283..3283 /label=stop polyA_site 3277..3290 /label=polya misc_feature 3250..3290 /label=BBa_B0012 terminator 3250..3290 /label=BBa_B0012 misc_feature 61..3290 /label=BBa_K173004 ORIGIN 1 gggttgatct ttgttgtcac tggatgtact gtacatccat acagtaactc actactagag 61 aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt 121 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 181 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 241 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg 301 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac 361 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt 421 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg 481 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg 541 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg 601 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac 661 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg 721 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat 781 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac 841 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct 901 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg 961 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt 1021 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc 1081 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc 1141 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg 1201 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg 1261 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc 1321 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta 1381 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg 1441 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc 1501 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc 1561 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg 1621 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg 1681 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg 1741 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag 1801 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc 1861 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg 1921 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg 1981 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac 2041 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct 2101 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa 2161 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac 2221 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg 2281 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat 2341 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa 2401 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt 2461 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg 2521 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg 2581 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg 2641 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg 2701 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag 2761 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc 2821 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc 2881 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc 2941 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc 3001 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt 3061 ggtggcgacg actcctggag cccgtcagta tcggcggaat ttcagctgag cgccggtcgc 3121 taccattacc agttggtctg gtgtcaaaaa taatactaga gccaggcatc aaataaaacg 3181 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 3241 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //