##gff-version 3
##sequence-region BBa_K862001 1 3290
BBa_K862001	.	promoter	1	52	.	+	0	ID=BBa_K518010;Name=BBa_K518010
BBa_K862001	.	engineered_region	61	3290	.	+	0	ID=BBa_K173004;Name=BBa_K173004
BBa_K862001	.	CDS	79	3153	.	+	0	ID=BBa_I732005;Name=BBa_I732005;Parent=BBa_K173004
BBa_K862001	.	point_mutation	3102	3102	.	+	0	ID=annotation1936869;Name=C->T;Parent=BBa_I732005
BBa_K862001	.	stop_codon	3151	3153	.	+	0	ID=annotation1936872;Name=STOP;Parent=BBa_I732005
BBa_K862001	.	start_codon	79	81	.	+	0	ID=annotation1936871;Name=START;Parent=BBa_I732005
BBa_K862001	.	CDS	79	3153	.	+	0	ID=annotation1936870;Name=lacZ;Parent=BBa_I732005
BBa_K862001	.	terminator	3162	3241	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K173004
BBa_K862001	.	stem_loop	3173	3216	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K862001	.	engineered_region	3162	3241	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K862001	.	terminator	3250	3290	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K173004
BBa_K862001	.	stop_codon	3283	3283	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K862001	.	stem_loop	3257	3276	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K862001	.	polyA_site	3277	3290	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K862001	.	engineered_region	3250	3290	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K862001	.	ribosome_entry_site	61	72	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K173004
BBa_K862001	.	conserved_region	65	68	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K862001
gggttgatctttgttgtcactggatgtactgtacatccatacagtaactcactactagagaaagaggagaaatactaga
tgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcg
ccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgc
agcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttc
ctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgaccta
tcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaa
agctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgct
gggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcct
cgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgt
gacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagcc
gcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcaggg
tgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtc
acactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgc
acaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgct
gctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggat
gagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaacc
atccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggt
gccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgat
cgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgct
ggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttg
cccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgcta
cctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatact
ggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatga
tgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggt
ctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttat
ccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtggcgct
ggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaa
ctaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaag
ccgggcacatcagcgcctggcagcagtggcgtctggcggaaaacctcagtgtgacgctccccgccgcgtcccacgccat
cccgcatctgaccaccagcgaaatggatttttgcatcgagctgggtaataagcgttggcaatttaaccgccagtcaggc
tttctttcacagatgtggattggcgataaaaaacaactgctgacgccgctgcgcgatcagttcacccgtgcaccgctgg
ataacgacattggcgtaagtgaagcgacccgcattgaccctaacgcctgggtcgaacgctggaaggcggcgggccatta
ccaggccgaagcagcgttgttgcagtgcacggcagatacacttgctgatgcggtgctgattacgaccgctcacgcgtgg
cagcatcaggggaaaaccttatttatcagccggaaaacctaccggattgatggtagtggtcaaatggcgattaccgttg
atgttgaagtggcgagcgatacaccgcatccggcgcggattggcctgaactgccagctggcgcaggtagcagagcgggt
aaactggctcggattagggccgcaagaaaactatcccgaccgccttactgccgcctgttttgaccgctgggatctgcca
ttgtcagacatgtataccccgtacgtcttcccgagcgaaaacggtctgcgctgcgggacgcgcgaattgaattatggcc
cacaccagtggcgcggcgacttccagttcaacatcagccgctacagtcaacagcaactgatggaaaccagccatcgcca
tctgctgcacgcggaagaaggcacatggctgaatatcgacggtttccatatggggattggtggcgacgactcctggagc
ccgtcagtatcggcggaatttcagctgagcgccggtcgctaccattaccagttggtctggtgtcaaaaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata