LOCUS BBa_K862002 3308 bp DNA linear 25-Nov-2024 DEFINITION precB-LacZ-doubleTerminator ACCESSION BBa_K862002 VERSION BBa_K862002.1 FEATURES Location/Qualifiers promoter 1..70 /label=BBa_K862003 misc_feature 83..86 /label=conserved RBS 79..90 /label=BBa_B0034 misc_feature 3120..3120 /label=C->T CDS 97..3171 /label=lacZ misc_feature 97..99 /label=START misc_feature 3169..3171 /label=STOP CDS 97..3171 /label=BBa_I732005 stem_loop 3191..3234 /label=stem_loop misc_feature 3180..3259 /label=BBa_B0010 terminator 3180..3259 /label=BBa_B0010 stem_loop 3275..3294 /label=T7 TE misc_feature 3301..3301 /label=stop polyA_site 3295..3308 /label=polya misc_feature 3268..3308 /label=BBa_B0012 terminator 3268..3308 /label=BBa_B0012 misc_feature 79..3308 /label=BBa_K173004 ORIGIN 1 cctgaaggct ggaaagtgtg ggagaacgtc agcgcgttgc agcaaacaat gcccctgatg 61 agtgaaaaga tactagagaa agaggagaaa tactagatga ccatgattac ggattcactg 121 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 181 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 241 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa 301 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc 361 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt 421 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt 481 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac 541 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg 601 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg 661 gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc 721 attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt 781 gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc 841 ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc 901 gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat 961 cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat 1021 ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc 1081 tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag 1141 ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg 1201 gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg 1261 cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg 1321 tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc 1381 gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat 1441 cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat 1501 cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa 1561 ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat 1621 gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct 1681 ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc 1741 ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc 1801 tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct 1861 tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc 1921 tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc 1981 cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc 2041 gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa 2101 gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag 2161 ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca 2221 tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt 2281 gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt 2341 tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag 2401 atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca 2461 ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc 2521 gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca 2581 gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa 2641 accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc 2701 gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag 2761 ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc 2821 gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc 2881 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc 2941 ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg 3001 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac 3061 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattt 3121 cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata atactagagc 3181 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3241 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 3301 cgtttata //