LOCUS       BBa_K863000             1587 bp    DNA     linear       22-Dec-2024
DEFINITION  bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS 
            tag
ACCESSION   BBa_K863000
VERSION     BBa_K863000.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=T7- Promotor BBa_I719005
     RBS             25..30
                     /label=RBS
     misc_feature    34..36
                     /label=ATG
     CDS             34..1556
                     /label=bpul
     misc_feature    834..834
                     /label=A (Original) T (Mutation)
     misc_feature    1557..1581
                     /label=6 x HIS
     misc_feature    1582..1584
                     /label=TAA
     misc_feature    1585..1587
                     /label=TAA
ORIGIN
        1 taatacgact cactataggg agagaggaga aaaatgaacc tagaaaaatt tgttgacgag
       61 ctgccaattc cagaagttgc gaagcccgtc aaaaagaacc caaaacaaac gtattatgaa
      121 atcgctatgg aggaggtatt tctaaaagtt catagagatc tgcccccaac caagctatgg
      181 acctataatg gcagtttgcc tggtccaacc attcatgcga atcgaaatga aaaagtcaaa
      241 gtgaaatgga tgaacaaatt gccacttaag cattttctac cggtcgatca caccattcac
      301 gaaggccatc atgatgaacc agaagttaaa accgtcgttc atttacatgg tggcgtcaca
      361 ccagcaagca gtgatggcta tccagaggct tggttttcac gagactttga agcaaccggc
      421 cccttctttg aacgggaggt gtacgaatac ccaaatcatc agcaagcctg cacattgtgg
      481 tatcacgatc atgcgatggc attgacacga ttaaatgtgt atgccggctt agctggattt
      541 tatttgatct cagatgcgtt tgaaaagtcg ctagaattac cgaagggtga gtatgatatt
      601 ccgctaatga tcatggaccg tacgtttcag gaggatggcg cactgtttta tccaagcagg
      661 ccaaacaaca caccagaaga cagtgacata ccagatccgt ctatcgtgcc tttcttttgc
      721 ggagaaacca ttttggtcaa tggaaaagta tggccgtatt tagaagtaga gccgcgaaaa
      781 tatcgttttc gtattttaaa tgcttccaat acaagaactt acgagctgca tcttgacaac
      841 gatgcgacga ttttgcaaat tggatctgat ggcggctttt taccaagacc tgttcaccat
      901 caatccttta gcattgctcc tgctgaacga tttgatgtca tcatcgattt ttcagcttac
      961 gaaaacaaaa cgatcaccct taaaaataaa gccggctgcg gacaggaagt aaatcctgaa
     1021 acagatgcaa acatcatgca atttaaagtc actcgaccgc taaaagggag agcacctaaa
     1081 acattacggc ctattttcaa accgcttcca ccacttcggc cttgtcgagc tgataaagag
     1141 cgtacgctca ctcttaccgg tacacaggat aaatacggcc gtcctatttt attgctagat
     1201 aaccaatttt ggaatgaccc tgtcacggaa aatcctcgtc ttggcagtgt ggaggtttgg
     1261 tctatcgtca atccaacaag gggcacacat cctattcatt tacaccttgt tcaattcaga
     1321 gtgatagaca gaagaccatt tgatactgag gtctatcaat cgacagggga cattgtgtat
     1381 acaggaccaa acgaagcacc tcccttacat gaacaaggct acaaggacac cattcaagcg
     1441 catgccggtg aagtcattcg gatcatcgct cgctttgttc catacagcgg caggtatgtg
     1501 tggcattgtc atatattaga gcacgaggat tatgacatga tgcggccgat ggatatcatc
     1561 cagcatcacc atcaccatca ctaataa
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