LOCUS       BBa_K863012             1471 bp    DNA     linear       16-Feb-2025
DEFINITION  tthl laccase from  T. thermophilus with constitutive promoter 
            J23100, RBS and His-tag
ACCESSION   BBa_K863012
VERSION     BBa_K863012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23100
     RBS             44..55
                     /label=BBa_J61101
     misc_feature    62..64
                     /label=ATG
     misc_feature    1448..1465
                     /label=6 x HIS
     misc_feature    1466..1468
                     /label=TAA
     misc_feature    1469..1471
                     /label=TAA
     CDS             62..1447
                     /label=tthl
     misc_feature    775..775
                     /label=g (original) --> a (mutation)
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagcttcta gagaaagaca ggacccacta
       61 gatgctggcg cgcaggagct ttctccaagc ggcggcgggc agtctggtcc tgggcctggc
      121 ccgggcccaa ggcccttcct tccccgagcc caaggtggtg cggagccagg gcggcctcct
      181 ctccctgaag ctttctgcca cccccacccc gcttgccctg gcggggcaaa gggccaccct
      241 cctcacctac ggggggagct ttcccgggcc caccctccgg gtccgcccca gggacacggt
      301 gcgcctcacc ctggaaaacc gccttcccga gcccaccaac ctccactggc acggcctgcc
      361 catctcccct aaggtggacg accccttcct ggagatcccc ccgggggaga gctggaccta
      421 cgagttcacc gttcccaagg agctggcagg taccttctgg taccaccccc acctgcacgg
      481 ccgggtagcc ccccagctct ttgccggcct cctgggagcc ctcgtggtgg aaagctccct
      541 ggacgccatc cccgagctca gggaggcgga ggagcacctc ctcgtcctga aggacctggc
      601 cctccagggc gggcgcccgg cgccgcacac ccccatggac tggatgaacg ggaaggaggg
      661 cgacctggtc ctggtgaacg gggccctgcg gcccaccctg gtggcccaga aggccaccct
      721 gaggcttcgc ctcctcaacg cctccaacgc ccgctactac cgcctggccc tgcaagacca
      781 ccccctttac ctcatcgccg ccgatggggg cttcctggaa gagcccctgg aggtgtccga
      841 gctcctcctg gccccaggag agcgggccga ggtcctggtg cgcttgcgga aagagggccg
      901 cttcctcctc caggccctgc cctacgaccg cggggccatg ggcatgatgg acatgggggg
      961 catggcccac gccatgcccc aagggccaag ccggcccgaa acccttcttt acctcatcgc
     1021 ccccaagaac cccaagccct tacccctgcc caaggcccta agccccttcc ccaccctgcc
     1081 cgcccccgtg gtcacccgcc gcctggtcct caccgaggac atgatggccg cccgcttctt
     1141 catcaacggc caggtctttg accacaggcg ggtggacctg aaggggcagg cccagacggt
     1201 ggaggtctgg gaggtggaaa accaggggga catggaccac cccttccacc tccacgtcca
     1261 ccccttccag gtcctctccg tgggcgggag gcctttcccc taccgggcct ggaaggatgt
     1321 ggtcaacctg aaggcgggcg aggtggccag gcttctggtt cccttgaggg aaaagggccg
     1381 gaccgttttt cactgccaca tcgtggagca cgaggaccgg gggatgatgg gagtcctcga
     1441 ggtgggtcat caccatcacc atcactaata a
//