LOCUS       BBa_K863103             1111 bp    DNA     linear       10-May-2024
DEFINITION  Cellulose binding Domain from Cellulomonas Fimi with Reporter GFP
ACCESSION   BBa_K863103
VERSION     BBa_K863103.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=T7 promoter
     misc_feature    1..32
                     /label=BBa_K525998
     misc_feature    1..364
                     /label=BBa_K863102
     misc_feature    21..32
                     /label=B0034
     RBS             24..27
                     /label=RBS
     misc_feature    35..37
                     /label=ATG
     misc_feature    38..49
                     /label=Exoglucanase
     misc_feature    38..364
                     /label=BBa_K863101
     CDS             50..349
                     /label=Cellulose binding domain (CBDcex)
     misc_feature    350..358
                     /label=Exoglucanase
     CDS             359..364
                     /label=GS-Linker
     misc_feature    365..370
                     /label=Freiburg-Scar
     misc_feature    371..1084
                     /label=BBa_E0040
     misc_feature    371..1111
                     /label=BBa_K863121
     misc_feature    1085..1102
                     /label=His-Tag
     misc_feature    1103..1108
                     /label=AgeI
     misc_feature    1109..1111
                     /label=TAA
ORIGIN
        1 taatacgact cactataggg aaagaggaga aataatgggt ccggccgggt gccaggtgct
       61 gtggggcgtc aaccagtgga acaccggctt caccgcgaac gtcaccgtga agaacacgtc
      121 ctccgctccg gtagacggct ggacgctcac gttcagcttc ccgtccggcc agcaggtcac
      181 ccaggcgtgg agctcgacgg tcacgcagtc cggctcggcc gtgacggtcc gcaacgcccc
      241 gtggaacggc tcgatcccgg cgggcggcac cgcgcagttc ggcttcaacg gctcgcacac
      301 gggcaccaac gccgcgccga cggcgttctc gctcaacggc acgccctgca cggtcggcgg
      361 cagcaccggc atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      421 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      481 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      541 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
      601 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      661 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      721 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      781 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
      841 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
      901 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
      961 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     1021 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     1081 caaacatcac catcaccatc acaccggtta a
//