LOCUS       BBa_K863113             1075 bp    DNA     linear       03-May-2024
DEFINITION  Cellulose binding Domain of C. cellulovorans cellulose binding 
            protein with Reporter GFP
ACCESSION   BBa_K863113
VERSION     BBa_K863113.1  
FEATURES             Location/Qualifiers
     misc_feature    1..328
                     /label=BBa_K863112
     misc_feature    1..32
                     /label=BBa_K525998
     promoter        1..20
                     /label=T7 promoter
     misc_feature    21..32
                     /label=B0034
     RBS             24..27
                     /label=RBS
     misc_feature    35..38
                     /label=ATG
     misc_feature    35..40
                     /label=Cellulose Binding Protein
     misc_feature    35..328
                     /label=BBa_K863111
     CDS             41..316
                     /label=Cellulose binding domain (CBDclos)
     misc_feature    317..322
                     /label=Cellulose Binding Protein
     CDS             323..328
                     /label=GS-Linker
     misc_feature    329..334
                     /label=Freiburg-Scar
     misc_feature    335..1075
                     /label=BBa_K863121
     misc_feature    335..1048
                     /label=BBa_E0040
     misc_feature    1049..1066
                     /label=His-Tag
     misc_feature    1068..1072
                     /label=AgeI
     misc_feature    1073..1075
                     /label=TAA
     misc_feature    112..112
                     /label=Silent mutation (A original)
ORIGIN
        1 taatacgact cactataggg aaagaggaga aataatgtca gttgaatttt acaactctaa
       61 caaatcagca caaacaaact caattacacc aataatcaaa attactaaca cgtctgacag
      121 tgatttaaat ttaaatgacg taaaagttag atattattac acaagtgatg gtacacaagg
      181 acaaactttc tggtgtgacc atgctggtgc attattagga aatagctatg ttgataacac
      241 tagcaaagtg acagcaaact tcgttaaaga aacagcaagc ccaacatcaa cctatgatac
      301 atatgttgaa tttggatttg caggcagcac cggcatgcgt aaaggagaag aacttttcac
      361 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      421 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      481 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
      541 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
      601 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
      661 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
      721 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
      781 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
      841 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
      901 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
      961 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     1021 tacacatggc atggatgaac tatacaaaca tcaccatcac catcacaccg gttaa
//