LOCUS       BBa_K863122              821 bp    DNA     linear       11-May-2024
DEFINITION  BBa_I13522 with His-tag
ACCESSION   BBa_K863122
VERSION     BBa_K863122.1  
FEATURES             Location/Qualifiers
     misc_feature    1..54
                     /label=BBa_R0040
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..800
                     /label=BBa_I13522
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     misc_feature    63..74
                     /label=BBa_B0034
     misc_feature    67..70
                     /label=conserved
     misc_feature    81..800
                     /label=BBa_E0040
     misc_feature    819..821
                     /label=stop
     CDS             801..812
                     /label=His-tag
     misc_feature    813..818
                     /label=AgeI
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaacatcac catcaccatc acaccggtta a
//