LOCUS BBa_K863204 4859 bp DNA linear 22-Nov-2024 DEFINITION shuttle vector pECPP11JS for recombination of genes of interest in yeast ACCESSION BBa_K863204 VERSION BBa_K863204.1 FEATURES Location/Qualifiers misc_feature 1..940 /label=5' AOX misc_feature 941..952 /label=Kosak sequence misc_feature 953..1215 /label=MFalpha misc_feature 1250..1531 /label=AOX1 terminator CDS 1615..4143 /label=his4 misc_feature 4184..4867 /label=3' AOX ORIGIN 1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt 541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 901 caacttgaga agatcaaaaa acaactaatt attcgaaacg cccgccgcca ccatgagatt 961 tccttcaatt tttactgctg ttttattcgc agcatcctcc gcattagctg ctccagtcaa 1021 cactacaaca gaagatgaaa cggcacaaat tccggctgaa gctgtcatcg gttactcaga 1081 tttagaaggg gatttcgatg ttgctgtttt gccattttcc aacagcacaa ataacgggtt 1141 attgtttata aatactacta ttgccagcat tgctgctaaa gaagaagggg tatctctcga 1201 gaaaagagag gctgaagcta cacgcaggtg gtatgtatca cctgcgtgtc ttgctagatt 1261 ctaatcaaga ggatgtcaga atgccatttg cctgagagat gcaggcttca tttttgatac 1321 ttttttattt gtaacctata tagtatagga ttttttttgt cattttgttt cttctcgtac 1381 gagcttgctc ctgatcagcc tatctcgcag ctgatgaata tcttgtggta ggggtttggg 1441 aaaatcattc gagtttgatg tttttcttgg tatttcccac tcctcttcag agtacagaag 1501 attaagtgag aagttcgttt gtgcaagctt aagatctcct gatgactgac tcactgataa 1561 taaaaatacg gcttcagaat ttctcaagac tacactcact gtccgacttc aagtatgaca 1621 tttcccttgc tacctgcata cgcaagtgtt gcagagtttg ataattcctt gagtttggta 1681 ggaaaagccg tgtttcccta tgctgctgac cagctgcaca acctgatcaa gttcactcaa 1741 tcgactgagc ttcaagttaa tgtgcaagtt gagtcatccg ttacagagga ccaatttgag 1801 gagctgatcg acaacttgct caagttgtac aataatggta tcaatgaagt gattttggac 1861 ctagatttgg cagaaagagt tgtccaaagg atcccaggcg ctagggttat ctataggacc 1921 ctggttgata aagttgcatc cttgcccgct aatgctagta tcgctgtgcc tttttcttct 1981 ccactgggcg atttgaaaag tttcactaat ggcggtagta gaactgttta tgctttttct 2041 gagaccgcaa agttggtaga tgtgacttcc actgttgctt ctggtataat ccccattatt 2101 gatgctcggc aattgactac tgaatacgaa ctttctgaag atgtcaaaaa gttccctgtc 2161 agtgaaattt tgttggcgtc tttgactact gaccgccccg atggtctatt cactactttg 2221 gtggctgact cttctaatta ctcgttgggc ctggtgtact cgtccaaaaa gtctattccg 2281 gaggctataa ggacacaaac tggagtctac caatctcgtc gtcacggttt gtggtataaa 2341 ggtgctacat ctggagcaac tcaaaagttg ctgggtatcg aattggattg tgatggagac 2401 tgcttgaaat ttgtggttga acaaacaggt gttggtttct gtcacttgga acgcacttcc 2461 tgttttggcc aatcaaaggg tcttagagcc atggaagcca ccttgtggga tcgtaagagc 2521 aatgctccag aaggttctta taccaaacgg ttatttgacg acgaagtttt gttgaacgct 2581 aaaattaggg aggaagctga tgaacttgca gaagctaaat ccaaggaaga tatagcctgg 2641 gaatgtgctg acttatttta ttttgcatta gttagatgtg ccaagtacgg cgtgacgttg 2701 gacgaggtgg agagaaacct ggatatgaag tccctaaagg tcactagaag gaaaggagat 2761 gccaagccag gatacaccaa ggaacaacct aaagaagaat ccaaacctaa agaagtccct 2821 tctgaaggtc gtattgaatt gtgcaaaatt gacgtttcta aggcctcctc acaagaaatt 2881 gaagatgccc ttcgtcgtcc tatccagaaa acggaacaga ttatggaatt agtcaaacca 2941 attgtcgaca atgttcgtca aaatggtgac aaagcccttt tagaactaac tgccaagttt 3001 gatggagtcg ctttgaagac acctgtgtta gaagctcctt tcccagagga acttatgcaa 3061 ttgccagata acgttaagag agccattgat ctctctatag ataacgtcag gaaattccat 3121 gaagctcaac tagcggagac gttgcaagtt gagacttgcc ctggtgtagt ctgctctcgt 3181 tttgcaagac ctattgagaa agttggcctc tatattcctg gtggaaccgc aattctgcct 3241 tccacttccc tgatgctggg tgttcctgcc aaagttgctg gttgcaaaga aattgttttt 3301 gcatctccac ctaagaagga tggtaccctt accccagaag tcatctacgt tgcccacaag 3361 gttggtgcta agtgtatcgt gctagcagga ggcgcccagg cagtagctgc tatggcttac 3421 ggaacagaaa ctgttcctaa gtgtgacaaa atatttggtc caggaaacca gttcgttact 3481 gctgccaaga tgatggttca aaatgacaca tcagccctgt gtagtattga catgcctgct 3541 gggccttctg aagttctagt tattgctgat aaatacgctg atccagattt cgttgtctca 3601 gaccttctgt ctcaagctga acatggtatt gattcccagg tgattctgtt ggctgtcgat 3661 atgacagaca aggagcttgc cagaattgaa gatgctgttc acaaccaagc tgtgcagttg 3721 ccaagggttg aaattgtacg caagtgtatt gcacactcta caaccctatc ggttgcaacc 3781 tacgagcagg ctttggaaat gtccaatcag tacgctcctg aacacttgat cctgcaaatc 3841 gagaatgctt cttatgttga tcaagtacaa cacgctggat ctgtgtttgt tggtgcctac 3901 tctccagaga gttgtggaga ttactcctcc ggtaccaacc acactttgcc aacgtacgga 3961 tatgcccgtc aatacagcgg agttaacact gcaaccttcc agaagttcat cacttcacaa 4021 gacgtaactc ctgagggact gaaacatatt ggccaagcag tgatggatct ggctgctgtt 4081 gaaggtctag atgctcaccg caatgctgtt aaggttcgta tggagaaact gggacttatt 4141 taattattta gagattttaa cttacattta gattcgatag atccgagtat ctatgattgg 4201 aagtatggga atggtgatac ccgcattctt cagtgtcttg aggtctccta tcagattatg 4261 cccaactaaa gcaaccggag gaggagattt catggtaaat ttctctgact tttggtcatc 4321 agtagactcg aactgtgaga ctatctcggt tatgacagca gaaatgtcct tcttggagac 4381 agtaaatgaa gtcccaccaa taaagaaatc cttgttatca ggaacaaact tcttgtttcg 4441 aactttttcg gtgccttgaa ctataaaatg tagagtggat atgtcgggta ggaatggagc 4501 gggcaaatgc ttaccttctg gaccttcaag aggtatgtag ggtttgtaga tactgatgcc 4561 aacttcagtg acaacgttgc tatttcgttc aaaccattcc gaatccagag aaatcaaagt 4621 tgtttgtcta ctattgatcc aagccagtgc ggtcttgaaa ctgacaatag tgtgctcgtg 4681 ttttgaggtc atctttgtat gaataaatct agtctttgat ctaaataatc ttgacgagcc 4741 agacgataat accaatctaa actctttaaa cgttaaagga caagtatgtc tgcctgtatt 4801 aaaccccaaa tcagctcgta gtctgatcct catcaacttg aggggcacta tcttgtttt //