LOCUS BBa_K863205 5553 bp DNA linear 16-Jun-2024 DEFINITION GFP integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast ACCESSION BBa_K863205 VERSION BBa_K863205.1 FEATURES Location/Qualifiers misc_feature 1..940 /label=5' AOX RBS 941..952 /label=Kosak misc_feature 953..1215 /label=MFalpha CDS 1220..1930 /label=GFP misc_feature 1934..2215 /label=AOX1 terminator CDS 2299..4827 /label=his4 misc_feature 4868..5551 /label=3' AOX1 ORIGIN 1 agagatctaa catccaaaga cgaaaggttg aatgaaacct ttttgccatc cgacatccac 61 aggtccattc tcacacataa gtgccaaacg caacaggagg ggatacacta gcagcagacc 121 gttgcaaacg caggacctcc actcctcttc tcctcaacac ccacttttgc catcgaaaaa 181 ccagcccagt tattgggctt gattggagct cgctcattcc aattccttct attaggctac 241 taacaccatg actttattag cctgtctatc ctggcccccc tggcgaggtt catgtttgtt 301 tatttccgaa tgcaacaagc tccgcattac acccgaacat cactccagat gagggctttc 361 tgagtgtggg gtcaaatagt ttcatgttcc ccaaatggcc caaaactgac agtttaaacg 421 ctgtcttgga acctaatatg acaaaagcgt gatctcatcc aagatgaact aagtttggtt 481 cgttgaaatg ctaacggcca gttggtcaaa aagaaacttc caaaagtcgg cataccgttt 541 gtcttgtttg gtattgattg acgaatgctc aaaaataatc tcattaatgc ttagcgcagt 601 ctctctatcg cttctgaacc ccggtgcacc tgtgccgaaa cgcaaatggg gaaacacccg 661 ctttttggat gattatgcat tgtctccaca ttgtatgctt ccaagattct ggtgggaata 721 ctgctgatag cctaacgttc atgatcaaaa tttaactgtt ctaaccccta cttgacagca 781 atatataaac agaaggaagc tgccctgtct taaacctttt tttttatcat cattattagc 841 ttactttcat aattgcgact ggttccaatt gacaagcttt tgattttaac gacttttaac 901 gacaacttga gaagatcaaa aaacaactaa ttattcgaaa cgcccgccgc caccatgaga 961 tttccttcaa tttttactgc tgttttattc gcagcatcct ccgcattagc tgctccagtc 1021 aacactacaa cagaagatga aacggcacaa attccggctg aagctgtcat cggttactca 1081 gatttagaag gggatttcga tgttgctgtt ttgccatttt ccaacagcac aaataacggg 1141 ttattgttta taaatactac tattgccagc attgctgcta aagaagaagg ggtatctctc 1201 gagaaaagag aggctgaagc tcgtaaagga gaagaacttt tcactggagt tgtcccaatt 1261 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa 1321 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct 1381 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac 1441 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 1501 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt 1561 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga 1621 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca 1681 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga 1741 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 1801 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag 1861 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat 1921 gaactataca aataacttgc tagattctaa tcaagaggat gtcagaatgc catttgcctg 1981 agagatgcag gcttcatttt tgatactttt ttatttgtaa cctatatagt ataggatttt 2041 ttttgtcatt ttgtttcttc tcgtacgagc ttgctcctga tcagcctatc tcgcagctga 2101 tgaatatctt gtggtagggg tttgggaaaa tcattcgagt ttgatgtttt tcttggtatt 2161 tcccactcct cttcagagta cagaagatta agtgagaagt tcgtttgtgc aagcttaaga 2221 tctcctgatg actgactcac tgataataaa aatacggctt cagaatttct caagactaca 2281 ctcactgtcc gacttcaagt atgacatttc ccttgctacc tgcatacgca agtgttgcag 2341 agtttgataa ttccttgagt ttggtaggaa aagccgtgtt tccctatgct gctgaccagc 2401 tgcacaacct gatcaagttc actcaatcga ctgagcttca agttaatgtg caagttgagt 2461 catccgttac agaggaccaa tttgaggagc tgatcgacaa cttgctcaag ttgtacaata 2521 atggtatcaa tgaagtgatt ttggacctag atttggcaga aagagttgtc caaaggatcc 2581 caggcgctag ggttatctat aggaccctgg ttgataaagt tgcatccttg cccgctaatg 2641 ctagtatcgc tgtgcctttt tcttctccac tgggcgattt gaaaagtttc actaatggcg 2701 gtagtagaac tgtttatgct ttttctgaga ccgcaaagtt ggtagatgtg acttccactg 2761 ttgcttctgg tataatcccc attattgatg ctcggcaatt gactactgaa tacgaacttt 2821 ctgaagatgt caaaaagttc cctgtcagtg aaattttgtt ggcgtctttg actactgacc 2881 gccccgatgg tctattcact actttggtgg ctgactcttc taattactcg ttgggcctgg 2941 tgtactcgtc caaaaagtct attccggagg ctataaggac acaaactgga gtctaccaat 3001 ctcgtcgtca cggtttgtgg tataaaggtg ctacatctgg agcaactcaa aagttgctgg 3061 gtatcgaatt ggattgtgat ggagactgct tgaaatttgt ggttgaacaa acaggtgttg 3121 gtttctgtca cttggaacgc acttcctgtt ttggccaatc aaagggtctt agagccatgg 3181 aagccacctt gtgggatcgt aagagcaatg ctccagaagg ttcttatacc aaacggttat 3241 ttgacgacga agttttgttg aacgctaaaa ttagggagga agctgatgaa cttgcagaag 3301 ctaaatccaa ggaagatata gcctgggaat gtgctgactt attttatttt gcattagtta 3361 gatgtgccaa gtacggcgtg acgttggacg aggtggagag aaacctggat atgaagtccc 3421 taaaggtcac tagaaggaaa ggagatgcca agccaggata caccaaggaa caacctaaag 3481 aagaatccaa acctaaagaa gtcccttctg aaggtcgtat tgaattgtgc aaaattgacg 3541 tttctaaggc ctcctcacaa gaaattgaag atgcccttcg tcgtcctatc cagaaaacgg 3601 aacagattat ggaattagtc aaaccaattg tcgacaatgt tcgtcaaaat ggtgacaaag 3661 cccttttaga actaactgcc aagtttgatg gagtcgcttt gaagacacct gtgttagaag 3721 ctcctttccc agaggaactt atgcaattgc cagataacgt taagagagcc attgatctct 3781 ctatagataa cgtcaggaaa ttccatgaag ctcaactagc ggagacgttg caagttgaga 3841 cttgccctgg tgtagtctgc tctcgttttg caagacctat tgagaaagtt ggcctctata 3901 ttcctggtgg aaccgcaatt ctgccttcca cttccctgat gctgggtgtt cctgccaaag 3961 ttgctggttg caaagaaatt gtttttgcat ctccacctaa gaaggatggt acccttaccc 4021 cagaagtcat ctacgttgcc cacaaggttg gtgctaagtg tatcgtgcta gcaggaggcg 4081 cccaggcagt agctgctatg gcttacggaa cagaaactgt tcctaagtgt gacaaaatat 4141 ttggtccagg aaaccagttc gttactgctg ccaagatgat ggttcaaaat gacacatcag 4201 ccctgtgtag tattgacatg cctgctgggc cttctgaagt tctagttatt gctgataaat 4261 acgctgatcc agatttcgtt gtctcagacc ttctgtctca agctgaacat ggtattgatt 4321 cccaggtgat tctgttggct gtcgatatga cagacaagga gcttgccaga attgaagatg 4381 ctgttcacaa ccaagctgtg cagttgccaa gggttgaaat tgtacgcaag tgtattgcac 4441 actctacaac cctatcggtt gcaacctacg agcaggcttt ggaaatgtcc aatcagtacg 4501 ctcctgaaca cttgatcctg caaatcgaga atgcttctta tgttgatcaa gtacaacacg 4561 ctggatctgt gtttgttggt gcctactctc cagagagttg tggagattac tcctccggta 4621 ccaaccacac tttgccaacg tacggatatg cccgtcaata cagcggagtt aacactgcaa 4681 ccttccagaa gttcatcact tcacaagacg taactcctga gggactgaaa catattggcc 4741 aagcagtgat ggatctggct gctgttgaag gtctagatgc tcaccgcaat gctgttaagg 4801 ttcgtatgga gaaactggga cttatttaat tatttagaga ttttaactta catttagatt 4861 cgatagatcc gagtatctat gattggaagt atgggaatgg tgatacccgc attcttcagt 4921 gtcttgaggt ctcctatcag attatgccca actaaagcaa ccggaggagg agatttcatg 4981 gtaaatttct ctgacttttg gtcatcagta gactcgaact gtgagactat ctcggttatg 5041 acagcagaaa tgtccttctt ggagacagta aatgaagtcc caccaataaa gaaatccttg 5101 ttatcaggaa caaacttctt gtttcgaact ttttcggtgc cttgaactat aaaatgtaga 5161 gtggatatgt cgggtaggaa tggagcgggc aaatgcttac cttctggacc ttcaagaggt 5221 atgtagggtt tgtagatact gatgccaact tcagtgacaa cgttgctatt tcgttcaaac 5281 cattccgaat ccagagaaat caaagttgtt tgtctactat tgatccaagc cagtgcggtc 5341 ttgaaactga caatagtgtg ctcgtgtttt gaggtcatct ttgtatgaat aaatctagtc 5401 tttgatctaa ataatcttga cgagccaagg cgataaatac ccaaatctaa aactctttta 5461 aaacgttaaa aggacaagta tgtctgcctg tattaaaccc caaatcagct cgtagtctga 5521 tcctcatcaa cttgaggggc actatcttgt ttt //