LOCUS BBa_K863207 6339 bp DNA linear 26-Nov-2024 DEFINITION tvel5 integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast ACCESSION BBa_K863207 VERSION BBa_K863207.1 FEATURES Location/Qualifiers RBS 941..952 /label=Kosak misc_feature 1..940 /label=5'AOX misc_feature 953..1215 /label=MFalpha1 CDS 1222..2721 /label=tvel5 misc_feature 2720..3001 /label=AOX1 terminator CDS 3085..5613 /label=his 4 misc_feature 5654..6337 /label=3' AOX ORIGIN 1 agagatctaa catccaaaga cgaaaggttg aatgaaacct ttttgccatc cgacatccac 61 aggtccattc tcacacataa gtgccaaacg caacaggagg ggatacacta gcagcagacc 121 gttgcaaacg caggacctcc actcctcttc tcctcaacac ccacttttgc catcgaaaaa 181 ccagcccagt tattgggctt gattggagct cgctcattcc aattccttct attaggctac 241 taacaccatg actttattag cctgtctatc ctggcccccc tggcgaggtt catgtttgtt 301 tatttccgaa tgcaacaagc tccgcattac acccgaacat cactccagat gagggctttc 361 tgagtgtggg gtcaaatagt ttcatgttcc ccaaatggcc caaaactgac agtttaaacg 421 ctgtcttgga acctaatatg acaaaagcgt gatctcatcc aagatgaact aagtttggtt 481 cgttgaaatg ctaacggcca gttggtcaaa aagaaacttc caaaagtcgg cataccgttt 541 gtcttgtttg gtattgattg acgaatgctc aaaaataatc tcattaatgc ttagcgcagt 601 ctctctatcg cttctgaacc ccggtgcacc tgtgccgaaa cgcaaatggg gaaacacccg 661 ctttttggat gattatgcat tgtctccaca ttgtatgctt ccaagattct ggtgggaata 721 ctgctgatag cctaacgttc atgatcaaaa tttaactgtt ctaaccccta cttgacagca 781 atatataaac agaaggaagc tgccctgtct taaacctttt tttttatcat cattattagc 841 ttactttcat aattgcgact ggttccaatt gacaagcttt tgattttaac gacttttaac 901 gacaacttga gaagatcaaa aaacaactaa ttattcgaaa cgcccgccgc caccatgaga 961 tttccttcaa tttttactgc tgttttattc gcagcatcct ccgcattagc tgctccagtc 1021 aacactacaa cagaagatga aacggcacaa attccggctg aagctgtcat cggttactca 1081 gatttagaag gggatttcga tgttgctgtt ttgccatttt ccaacagcac aaataacggg 1141 ttattgttta taaatactac tattgccagc attgctgcta aagaagaagg ggtatctctc 1201 gagaaaagag aggctgaagc tggtatcggt cctgtcgccg acctcaccat caccaacgca 1261 gcggtcagcc ccgacgggtt ttctcgccag gccgtcgtcg tgaacggcgg cacccctggc 1321 cctctcatca ccggtaacat gggggatcgc ttccagctca atgtcatcga caacctcacg 1381 aaccacacga tgctgaagag caccagtatt cactggcacg gtttcttcca gaagggcacc 1441 aactgggccg acggtcccgc cttcatcaac cagtgcccga tctcatctgg ccactcgttc 1501 ctgtatgact tccaggttcc tgaccaggct ggcaccttct ggtaccacag tcacttgtcc 1561 acgcagtact gtgatggtct gaggggtccg ttcgttgttt acgacccgaa tgacccggcc 1621 gccgacctgt acgacgtcga caacgacgac acggtcatta cccttgcgga ttggtaccac 1681 gtcgccgcga agctgggccc cgcattccct ctcggcgccg acgccactct catcaacggt 1741 aagggacgct cccccagcac gaccaccgcg gacctcactg ttatcagcgt cactccgggt 1801 aaacgttacc gtttccgcct ggtgtccctg tcgtgcgacc ccaaccacac cttcagcatc 1861 gatggccaca acatgacgat catcgagacc gactcgatca acacggcgcc cctcgtggtc 1921 gactccattc agatcttcgc tgcccagcgt tactccttcg tgctcgaggc caaccaggcc 1981 gtcgacaact actggattcg cgccaacccg agcttcggta acgtcgggtt caccggcggc 2041 atcaactcgg ctatcctccg ctatgatggc gccgctgcca tcgagcccac caccacgcag 2101 accacttcga ccgagccgct caacgaggtc aacctgcacc cgctggttgc caccgctgtc 2161 cctggctctc ccgctgcggg tggtgttgac ctggccatca atatggcgtt caacttcaat 2221 ggcaccaact tcttcatcaa cggcgcgtct ttcacgcccc cgaccgtgcc tgtcctcctc 2281 cagatcatca gcggcgcgca gaacgcgcag gacctcctgc cctccggcag cgtatactcg 2341 ctcccctcga acgccgacat cgagatctcc ttccccgcca ccgccgctgc ccccggtgcg 2401 ccccacccct tccacttgca cgggcacgcg ttcgcggtcg tccgcagcgc cggcagcacg 2461 gtctacaact acgacaaccc catcttccgc gacgtcgtca gcacggggac gcctgcggcc 2521 ggtgacaacg tcaccatccg cttccgcacc gacaaccccg gcccgtggtt tctccactgc 2581 cacatcgact tccacctcga ggccggcttc gccgtcgtgt ttgcggagga catccccgat 2641 gtcgcatcgg cgaaccccgt cccccaggcg tggtccgacc tctgcccgac ctacgacgcg 2701 cgcgacccga gcgaccagta acttgctaga ttctaatcaa gaggatgtca gaatgccatt 2761 tgcctgagag atgcaggctt catttttgat acttttttat ttgtaaccta tatagtatag 2821 gatttttttt gtcattttgt ttcttctcgt acgagcttgc tcctgatcag cctatctcgc 2881 agctgatgaa tatcttgtgg taggggtttg ggaaaatcat tcgagtttga tgtttttctt 2941 ggtatttccc actcctcttc agagtacaga agattaagtg agaagttcgt ttgtgcaagc 3001 ttaagatctc ctgatgactg actcactgat aataaaaata cggcttcaga atttctcaag 3061 actacactca ctgtccgact tcaagtatga catttccctt gctacctgca tacgcaagtg 3121 ttgcagagtt tgataattcc ttgagtttgg taggaaaagc cgtgtttccc tatgctgctg 3181 accagctgca caacctgatc aagttcactc aatcgactga gcttcaagtt aatgtgcaag 3241 ttgagtcatc cgttacagag gaccaatttg aggagctgat cgacaacttg ctcaagttgt 3301 acaataatgg tatcaatgaa gtgattttgg acctagattt ggcagaaaga gttgtccaaa 3361 ggatcccagg cgctagggtt atctatagga ccctggttga taaagttgca tccttgcccg 3421 ctaatgctag tatcgctgtg cctttttctt ctccactggg cgatttgaaa agtttcacta 3481 atggcggtag tagaactgtt tatgcttttt ctgagaccgc aaagttggta gatgtgactt 3541 ccactgttgc ttctggtata atccccatta ttgatgctcg gcaattgact actgaatacg 3601 aactttctga agatgtcaaa aagttccctg tcagtgaaat tttgttggcg tctttgacta 3661 ctgaccgccc cgatggtcta ttcactactt tggtggctga ctcttctaat tactcgttgg 3721 gcctggtgta ctcgtccaaa aagtctattc cggaggctat aaggacacaa actggagtct 3781 accaatctcg tcgtcacggt ttgtggtata aaggtgctac atctggagca actcaaaagt 3841 tgctgggtat cgaattggat tgtgatggag actgcttgaa atttgtggtt gaacaaacag 3901 gtgttggttt ctgtcacttg gaacgcactt cctgttttgg ccaatcaaag ggtcttagag 3961 ccatggaagc caccttgtgg gatcgtaaga gcaatgctcc agaaggttct tataccaaac 4021 ggttatttga cgacgaagtt ttgttgaacg ctaaaattag ggaggaagct gatgaacttg 4081 cagaagctaa atccaaggaa gatatagcct gggaatgtgc tgacttattt tattttgcat 4141 tagttagatg tgccaagtac ggcgtgacgt tggacgaggt ggagagaaac ctggatatga 4201 agtccctaaa ggtcactaga aggaaaggag atgccaagcc aggatacacc aaggaacaac 4261 ctaaagaaga atccaaacct aaagaagtcc cttctgaagg tcgtattgaa ttgtgcaaaa 4321 ttgacgtttc taaggcctcc tcacaagaaa ttgaagatgc ccttcgtcgt cctatccaga 4381 aaacggaaca gattatggaa ttagtcaaac caattgtcga caatgttcgt caaaatggtg 4441 acaaagccct tttagaacta actgccaagt ttgatggagt cgctttgaag acacctgtgt 4501 tagaagctcc tttcccagag gaacttatgc aattgccaga taacgttaag agagccattg 4561 atctctctat agataacgtc aggaaattcc atgaagctca actagcggag acgttgcaag 4621 ttgagacttg ccctggtgta gtctgctctc gttttgcaag acctattgag aaagttggcc 4681 tctatattcc tggtggaacc gcaattctgc cttccacttc cctgatgctg ggtgttcctg 4741 ccaaagttgc tggttgcaaa gaaattgttt ttgcatctcc acctaagaag gatggtaccc 4801 ttaccccaga agtcatctac gttgcccaca aggttggtgc taagtgtatc gtgctagcag 4861 gaggcgccca ggcagtagct gctatggctt acggaacaga aactgttcct aagtgtgaca 4921 aaatatttgg tccaggaaac cagttcgtta ctgctgccaa gatgatggtt caaaatgaca 4981 catcagccct gtgtagtatt gacatgcctg ctgggccttc tgaagttcta gttattgctg 5041 ataaatacgc tgatccagat ttcgttgtct cagaccttct gtctcaagct gaacatggta 5101 ttgattccca ggtgattctg ttggctgtcg atatgacaga caaggagctt gccagaattg 5161 aagatgctgt tcacaaccaa gctgtgcagt tgccaagggt tgaaattgta cgcaagtgta 5221 ttgcacactc tacaacccta tcggttgcaa cctacgagca ggctttggaa atgtccaatc 5281 agtacgctcc tgaacacttg atcctgcaaa tcgagaatgc ttcttatgtt gatcaagtac 5341 aacacgctgg atctgtgttt gttggtgcct actctccaga gagttgtgga gattactcct 5401 ccggtaccaa ccacactttg ccaacgtacg gatatgcccg tcaatacagc ggagttaaca 5461 ctgcaacctt ccagaagttc atcacttcac aagacgtaac tcctgaggga ctgaaacata 5521 ttggccaagc agtgatggat ctggctgctg ttgaaggtct agatgctcac cgcaatgctg 5581 ttaaggttcg tatggagaaa ctgggactta tttaattatt tagagatttt aacttacatt 5641 tagattcgat agatccgagt atctatgatt ggaagtatgg gaatggtgat acccgcattc 5701 ttcagtgtct tgaggtctcc tatcagatta tgcccaacta aagcaaccgg aggaggagat 5761 ttcatggtaa atttctctga cttttggtca tcagtagact cgaactgtga gactatctcg 5821 gttatgacag cagaaatgtc cttcttggag acagtaaatg aagtcccacc aataaagaaa 5881 tccttgttat caggaacaaa cttcttgttt cgaacttttt cggtgccttg aactataaaa 5941 tgtagagtgg atatgtcggg taggaatgga gcgggcaaat gcttaccttc tggaccttca 6001 agaggtatgt agggtttgta gatactgatg ccaacttcag tgacaacgtt gctatttcgt 6061 tcaaaccatt ccgaatccag agaaatcaaa gttgtttgtc tactattgat ccaagccagt 6121 gcggtcttga aactgacaat agtgtgctcg tgttttgagg tcatctttgt atgaataaat 6181 ctagtctttg atctaaataa tcttgacgag ccaaggcgat aaatacccaa atctaaaact 6241 cttttaaaac gttaaaagga caagtatgtc tgcctgtatt aaaccccaaa tcagctcgta 6301 gtctgatcct catcaacttg aggggcacta tcttgtttt //