LOCUS BBa_K863208 6333 bp DNA linear 29-Sep-2024 DEFINITION tvel35 integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast ACCESSION BBa_K863208 VERSION BBa_K863208.1 FEATURES Location/Qualifiers misc_feature 1..940 /label=5' AOX RBS 941..952 /label=Kozak sequence misc_feature 953..1215 /label=MFalpha1 misc_feature 5648..6331 /label=3' AOX CDS 1220..2710 /label=tvel35 CDS 3079..5607 /label=his4 misc_feature 2714..2995 /label=AOX1 terminator ORIGIN 1 agagatctaa catccaaaga cgaaaggttg aatgaaacct ttttgccatc cgacatccac 61 aggtccattc tcacacataa gtgccaaacg caacaggagg ggatacacta gcagcagacc 121 gttgcaaacg caggacctcc actcctcttc tcctcaacac ccacttttgc catcgaaaaa 181 ccagcccagt tattgggctt gattggagct cgctcattcc aattccttct attaggctac 241 taacaccatg actttattag cctgtctatc ctggcccccc tggcgaggtt catgtttgtt 301 tatttccgaa tgcaacaagc tccgcattac acccgaacat cactccagat gagggctttc 361 tgagtgtggg gtcaaatagt ttcatgttcc ccaaatggcc caaaactgac agtttaaacg 421 ctgtcttgga acctaatatg acaaaagcgt gatctcatcc aagatgaact aagtttggtt 481 cgttgaaatg ctaacggcca gttggtcaaa aagaaacttc caaaagtcgg cataccgttt 541 gtcttgtttg gtattgattg acgaatgctc aaaaataatc tcattaatgc ttagcgcagt 601 ctctctatcg cttctgaacc ccggtgcacc tgtgccgaaa cgcaaatggg gaaacacccg 661 ctttttggat gattatgcat tgtctccaca ttgtatgctt ccaagattct ggtgggaata 721 ctgctgatag cctaacgttc atgatcaaaa tttaactgtt ctaaccccta cttgacagca 781 atatataaac agaaggaagc tgccctgtct taaacctttt tttttatcat cattattagc 841 ttactttcat aattgcgact ggttccaatt gacaagcttt tgattttaac gacttttaac 901 gacaacttga gaagatcaaa aaacaactaa ttattcgaaa cgcccgccgc caccatgaga 961 tttccttcaa tttttactgc tgttttattc gcagcatcct ccgcattagc tgctccagtc 1021 aacactacaa cagaagatga aacggcacaa attccggctg aagctgtcat cggttactca 1081 gatttagaag gggatttcga tgttgctgtt ttgccatttt ccaacagcac aaataacggg 1141 ttattgttta taaatactac tattgccagc attgctgcta aagaagaagg ggtatctctc 1201 gagaaaagag aggctgaagc tgccataggg cctgtggcgg acctgaccct caccaatgcc 1261 caggtcagcc ccgatggctt cgctcgcgag gccgtcgtgg tgaacggtat cacccctgcc 1321 cctctcatca caggcaataa gggcgatcga ttccagctca atgtcatcga ccagttgaca 1381 aatcatacca tgttgaaaac atctagtatt cattggcacg gcttcttcca gcaaggcacg 1441 aactgggccg atggtcccgc gttcgtgaac cagtgtccca tcgcttcggg ccactcgttc 1501 ttgtatgact ttcaagttcc cgaccaagca gggaccttct ggtaccatag ccatctctcc 1561 acgcaatact gcgatggttt gagggggcct ttcgtcgtct acgaccccaa cgatcctcac 1621 gctagcctgt atgacattga taacgacgac actgtcatta cgctggctga ttggtatcac 1681 gttgctgcca agctcggacc tcgcttccca tttggctccg attcaaccct tatcaatgga 1741 cttggtcgaa ccactggcat agcaccgtcc gacttggcag ttatcaaggt cacgcagggc 1801 aagcgctacc gcttccgctt ggtgtcgctt tcttgcgatc cgaaccatac attcagcatt 1861 gataatcaca caatgactat aattgaggcg gactcgatca acactcaacc cctagaggtt 1921 gattcaatcc agatttttgc cgcgcagcgc tactccttcg tgctggatgc tagccagccg 1981 gtggataact actggatccg cgcaaaccct gtcttcggaa acacaggttt tgctggtgga 2041 atcaattctg ccatcctgcg ttatgatggc gcacccgaga tcgagcctac gtctgtccag 2101 actactccta cgaagcctct gaacgaggtc gacttgcatc ctctctcgcc tatgcctgtg 2161 cctggcagcc ccgagcccgg aggtgtcgac aagcctctga acttggtctt caacttcaac 2221 ggcaccaact tcttcatcaa cgaccacacc tttgtcccgc cgtctgtccc agtcttgcta 2281 caaatcctca gtggggcgca ggcggctcag gacctggtcc cggagggcag cgtgttcgtt 2341 cttcccagca actcgtccat tgagatatcc ttccctgcca ctgccaatgc ccctggattc 2401 ccccatccgt tccacttgca cggtcacgcc ttcgctgtcg tccggagcgc cgggagcagc 2461 gtctacaact acgacaaccc gatcttccgc gacgtcgtca gcaccggcca gcccggcgac 2521 aacgtcacga ttcgcttcga gaccaataac ccaggcccgt ggttcctcca ctgccacatc 2581 gacttccacc tcgacgcagg ctttgctgta gtcatggccg aggacactcc ggacaccaag 2641 gccgcgaacc ctgttcctca ggcgtggtcg gacttgtgcc ccatctatga tgcacttgac 2701 cccagcgacc tctgacttgc tagattctaa tcaagaggat gtcagaatgc catttgcctg 2761 agagatgcag gcttcatttt tgatactttt ttatttgtaa cctatatagt ataggatttt 2821 ttttgtcatt ttgtttcttc tcgtacgagc ttgctcctga tcagcctatc tcgcagctga 2881 tgaatatctt gtggtagggg tttgggaaaa tcattcgagt ttgatgtttt tcttggtatt 2941 tcccactcct cttcagagta cagaagatta agtgagaagt tcgtttgtgc aagcttaaga 3001 tctcctgatg actgactcac tgataataaa aatacggctt cagaatttct caagactaca 3061 ctcactgtcc gacttcaagt atgacatttc ccttgctacc tgcatacgca agtgttgcag 3121 agtttgataa ttccttgagt ttggtaggaa aagccgtgtt tccctatgct gctgaccagc 3181 tgcacaacct gatcaagttc actcaatcga ctgagcttca agttaatgtg caagttgagt 3241 catccgttac agaggaccaa tttgaggagc tgatcgacaa cttgctcaag ttgtacaata 3301 atggtatcaa tgaagtgatt ttggacctag atttggcaga aagagttgtc caaaggatcc 3361 caggcgctag ggttatctat aggaccctgg ttgataaagt tgcatccttg cccgctaatg 3421 ctagtatcgc tgtgcctttt tcttctccac tgggcgattt gaaaagtttc actaatggcg 3481 gtagtagaac tgtttatgct ttttctgaga ccgcaaagtt ggtagatgtg acttccactg 3541 ttgcttctgg tataatcccc attattgatg ctcggcaatt gactactgaa tacgaacttt 3601 ctgaagatgt caaaaagttc cctgtcagtg aaattttgtt ggcgtctttg actactgacc 3661 gccccgatgg tctattcact actttggtgg ctgactcttc taattactcg ttgggcctgg 3721 tgtactcgtc caaaaagtct attccggagg ctataaggac acaaactgga gtctaccaat 3781 ctcgtcgtca cggtttgtgg tataaaggtg ctacatctgg agcaactcaa aagttgctgg 3841 gtatcgaatt ggattgtgat ggagactgct tgaaatttgt ggttgaacaa acaggtgttg 3901 gtttctgtca cttggaacgc acttcctgtt ttggccaatc aaagggtctt agagccatgg 3961 aagccacctt gtgggatcgt aagagcaatg ctccagaagg ttcttatacc aaacggttat 4021 ttgacgacga agttttgttg aacgctaaaa ttagggagga agctgatgaa cttgcagaag 4081 ctaaatccaa ggaagatata gcctgggaat gtgctgactt attttatttt gcattagtta 4141 gatgtgccaa gtacggcgtg acgttggacg aggtggagag aaacctggat atgaagtccc 4201 taaaggtcac tagaaggaaa ggagatgcca agccaggata caccaaggaa caacctaaag 4261 aagaatccaa acctaaagaa gtcccttctg aaggtcgtat tgaattgtgc aaaattgacg 4321 tttctaaggc ctcctcacaa gaaattgaag atgcccttcg tcgtcctatc cagaaaacgg 4381 aacagattat ggaattagtc aaaccaattg tcgacaatgt tcgtcaaaat ggtgacaaag 4441 cccttttaga actaactgcc aagtttgatg gagtcgcttt gaagacacct gtgttagaag 4501 ctcctttccc agaggaactt atgcaattgc cagataacgt taagagagcc attgatctct 4561 ctatagataa cgtcaggaaa ttccatgaag ctcaactagc ggagacgttg caagttgaga 4621 cttgccctgg tgtagtctgc tctcgttttg caagacctat tgagaaagtt ggcctctata 4681 ttcctggtgg aaccgcaatt ctgccttcca cttccctgat gctgggtgtt cctgccaaag 4741 ttgctggttg caaagaaatt gtttttgcat ctccacctaa gaaggatggt acccttaccc 4801 cagaagtcat ctacgttgcc cacaaggttg gtgctaagtg tatcgtgcta gcaggaggcg 4861 cccaggcagt agctgctatg gcttacggaa cagaaactgt tcctaagtgt gacaaaatat 4921 ttggtccagg aaaccagttc gttactgctg ccaagatgat ggttcaaaat gacacatcag 4981 ccctgtgtag tattgacatg cctgctgggc cttctgaagt tctagttatt gctgataaat 5041 acgctgatcc agatttcgtt gtctcagacc ttctgtctca agctgaacat ggtattgatt 5101 cccaggtgat tctgttggct gtcgatatga cagacaagga gcttgccaga attgaagatg 5161 ctgttcacaa ccaagctgtg cagttgccaa gggttgaaat tgtacgcaag tgtattgcac 5221 actctacaac cctatcggtt gcaacctacg agcaggcttt ggaaatgtcc aatcagtacg 5281 ctcctgaaca cttgatcctg caaatcgaga atgcttctta tgttgatcaa gtacaacacg 5341 ctggatctgt gtttgttggt gcctactctc cagagagttg tggagattac tcctccggta 5401 ccaaccacac tttgccaacg tacggatatg cccgtcaata cagcggagtt aacactgcaa 5461 ccttccagaa gttcatcact tcacaagacg taactcctga gggactgaaa catattggcc 5521 aagcagtgat ggatctggct gctgttgaag gtctagatgc tcaccgcaat gctgttaagg 5581 ttcgtatgga gaaactggga cttatttaat tatttagaga ttttaactta catttagatt 5641 cgatagatcc gagtatctat gattggaagt atgggaatgg tgatacccgc attcttcagt 5701 gtcttgaggt ctcctatcag attatgccca actaaagcaa ccggaggagg agatttcatg 5761 gtaaatttct ctgacttttg gtcatcagta gactcgaact gtgagactat ctcggttatg 5821 acagcagaaa tgtccttctt ggagacagta aatgaagtcc caccaataaa gaaatccttg 5881 ttatcaggaa caaacttctt gtttcgaact ttttcggtgc cttgaactat aaaatgtaga 5941 gtggatatgt cgggtaggaa tggagcgggc aaatgcttac cttctggacc ttcaagaggt 6001 atgtagggtt tgtagatact gatgccaact tcagtgacaa cgttgctatt tcgttcaaac 6061 cattccgaat ccagagaaat caaagttgtt tgtctactat tgatccaagc cagtgcggtc 6121 ttgaaactga caatagtgtg ctcgtgtttt gaggtcatct ttgtatgaat aaatctagtc 6181 tttgatctaa ataatcttga cgagccaagg cgataaatac ccaaatctaa aactctttta 6241 aaacgttaaa aggacaagta tgtctgcctg tattaaaccc caaatcagct cgtagtctga 6301 tcctcatcaa cttgaggggc actatcttgt ttt //