LOCUS BBa_K863209 6396 bp DNA linear 01-Jul-2024 DEFINITION BBa_500002 integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast ACCESSION BBa_K863209 VERSION BBa_K863209.1 FEATURES Location/Qualifiers misc_feature 1..940 /label=5' AOX RBS 941..952 /label=Kozak sequence misc_feature 953..1215 /label=MFalpha1 misc_feature 1220..2776 /label=BBa_K500002 misc_feature 2777..3058 /label=AOX1 terminator CDS 5711..6394 /label=3' AOX misc_feature 3142..5670 /label=his4 ORIGIN 1 agagatctaa catccaaaga cgaaaggttg aatgaaacct ttttgccatc cgacatccac 61 aggtccattc tcacacataa gtgccaaacg caacaggagg ggatacacta gcagcagacc 121 gttgcaaacg caggacctcc actcctcttc tcctcaacac ccacttttgc catcgaaaaa 181 ccagcccagt tattgggctt gattggagct cgctcattcc aattccttct attaggctac 241 taacaccatg actttattag cctgtctatc ctggcccccc tggcgaggtt catgtttgtt 301 tatttccgaa tgcaacaagc tccgcattac acccgaacat cactccagat gagggctttc 361 tgagtgtggg gtcaaatagt ttcatgttcc ccaaatggcc caaaactgac agtttaaacg 421 ctgtcttgga acctaatatg acaaaagcgt gatctcatcc aagatgaact aagtttggtt 481 cgttgaaatg ctaacggcca gttggtcaaa aagaaacttc caaaagtcgg cataccgttt 541 gtcttgtttg gtattgattg acgaatgctc aaaaataatc tcattaatgc ttagcgcagt 601 ctctctatcg cttctgaacc ccggtgcacc tgtgccgaaa cgcaaatggg gaaacacccg 661 ctttttggat gattatgcat tgtctccaca ttgtatgctt ccaagattct ggtgggaata 721 ctgctgatag cctaacgttc atgatcaaaa tttaactgtt ctaaccccta cttgacagca 781 atatataaac agaaggaagc tgccctgtct taaacctttt tttttatcat cattattagc 841 ttactttcat aattgcgact ggttccaatt gacaagcttt tgattttaac gacttttaac 901 gacaacttga gaagatcaaa aaacaactaa ttattcgaaa cgcccgccgc caccatgaga 961 tttccttcaa tttttactgc tgttttattc gcagcatcct ccgcattagc tgctccagtc 1021 aacactacaa cagaagatga aacggcacaa attccggctg aagctgtcat cggttactca 1081 gatttagaag gggatttcga tgttgctgtt ttgccatttt ccaacagcac aaataacggg 1141 ttattgttta taaatactac tattgccagc attgctgcta aagaagaagg ggtatctctc 1201 gagaaaagag aggctgaagc tggtttacaa agattctcct tcttcgttac cttggctttg 1261 gttgctagat ctttggctgc tattggtcca gttgcttctt tggttgttgc taatgctcca 1321 gtttctccag atggtttttt gagagatgcc atcgttgtta atggtgttgt tccatctcca 1381 ttgattactg gtaagaaggg tgacagattc caattgaacg ttgttgatac cttgaccaac 1441 cattctatgt tgaagtctac ctccattcat tggcatggtt tttttcaagc tggtactaat 1501 tgggctgatg gtccagcttt tgttaatcaa tgtccaattg cttccggtca ttccttcttg 1561 tacgattttc atgttccaga tcaagccggt actttctggt atcattctca tttgtccacc 1621 caatactgtg atggtttgag aggtccattc gttgtttacg atccaaaaga tccacatgct 1681 tccagatacg atgttgataa cgaatctacc gttatcactt tgaccgattg gtatcatact 1741 gctgctagat tgggtccaag atttccattg ggtgctgatg ctactttgat taacggtttg 1801 ggtagatctg cttctactcc aactgctgct ttggctgtta ttaacgttca acatggtaag 1861 agatacagat tcagattggt ttccatttcc tgcgatccaa actacacctt ttccattgat 1921 ggtcataact tgaccgttat cgaagttgat ggtattaact cccaaccttt gttggttgat 1981 tccattcaaa ttttcgccgc tcaaagatac tcctttgttt tgaacgccaa tcaaaccgtt 2041 ggtaattatt gggttagagc taatccaaac ttcggtactg ttggttttgc tggtggtatc 2101 aattccgcta ttttgagata tcaaggtgct ccagttgctg aacctactac tactcaaact 2161 acctctgtta tcccattgat cgaaactaac ttgcatccat tggctagaat gccagttcca 2221 ggttctccaa ctccaggtgg tgttgataag gctttgaatt tggctttcaa cttcaacggt 2281 acaaacttct tcattaacaa cgctactttc actccaccaa ctgttccagt tttgttgcaa 2341 attttgtctg gtgctcaaac cgctcaagat ttgttgccag ctggttctgt ttatccattg 2401 ccagctcatt ccaccattga aattactttg ccagctactg ctttggctcc aggtgctcca 2461 catccatttc acttgcatgg tcatgctttt gctgttgtta gatctgctgg ttctactact 2521 tacaactaca acgatccaat cttcagagat gttgtttcta ctggtactcc agctgctggt 2581 gataatgtta ctattagatt ccaaaccgac aatccaggtc catggttttt acattgccat 2641 atcgatttcc atttggatgc tggtttcgct atcgtttttg ctgaagatgt tgctgatgtt 2701 aaggctgcta atccagttcc aaaagcttgg tctgatttgt gtccaatcta cgatggtttg 2761 tccgaagcta atcaataact tgctagattc taatcaagag gatgtcagaa tgccatttgc 2821 ctgagagatg caggcttcat ttttgatact tttttatttg taacctatat agtataggat 2881 tttttttgtc attttgtttc ttctcgtacg agcttgctcc tgatcagcct atctcgcagc 2941 tgatgaatat cttgtggtag gggtttggga aaatcattcg agtttgatgt ttttcttggt 3001 atttcccact cctcttcaga gtacagaaga ttaagtgaga agttcgtttg tgcaagctta 3061 agatctcctg atgactgact cactgataat aaaaatacgg cttcagaatt tctcaagact 3121 acactcactg tccgacttca agtatgacat ttcccttgct acctgcatac gcaagtgttg 3181 cagagtttga taattccttg agtttggtag gaaaagccgt gtttccctat gctgctgacc 3241 agctgcacaa cctgatcaag ttcactcaat cgactgagct tcaagttaat gtgcaagttg 3301 agtcatccgt tacagaggac caatttgagg agctgatcga caacttgctc aagttgtaca 3361 ataatggtat caatgaagtg attttggacc tagatttggc agaaagagtt gtccaaagga 3421 tcccaggcgc tagggttatc tataggaccc tggttgataa agttgcatcc ttgcccgcta 3481 atgctagtat cgctgtgcct ttttcttctc cactgggcga tttgaaaagt ttcactaatg 3541 gcggtagtag aactgtttat gctttttctg agaccgcaaa gttggtagat gtgacttcca 3601 ctgttgcttc tggtataatc cccattattg atgctcggca attgactact gaatacgaac 3661 tttctgaaga tgtcaaaaag ttccctgtca gtgaaatttt gttggcgtct ttgactactg 3721 accgccccga tggtctattc actactttgg tggctgactc ttctaattac tcgttgggcc 3781 tggtgtactc gtccaaaaag tctattccgg aggctataag gacacaaact ggagtctacc 3841 aatctcgtcg tcacggtttg tggtataaag gtgctacatc tggagcaact caaaagttgc 3901 tgggtatcga attggattgt gatggagact gcttgaaatt tgtggttgaa caaacaggtg 3961 ttggtttctg tcacttggaa cgcacttcct gttttggcca atcaaagggt cttagagcca 4021 tggaagccac cttgtgggat cgtaagagca atgctccaga aggttcttat accaaacggt 4081 tatttgacga cgaagttttg ttgaacgcta aaattaggga ggaagctgat gaacttgcag 4141 aagctaaatc caaggaagat atagcctggg aatgtgctga cttattttat tttgcattag 4201 ttagatgtgc caagtacggc gtgacgttgg acgaggtgga gagaaacctg gatatgaagt 4261 ccctaaaggt cactagaagg aaaggagatg ccaagccagg atacaccaag gaacaaccta 4321 aagaagaatc caaacctaaa gaagtccctt ctgaaggtcg tattgaattg tgcaaaattg 4381 acgtttctaa ggcctcctca caagaaattg aagatgccct tcgtcgtcct atccagaaaa 4441 cggaacagat tatggaatta gtcaaaccaa ttgtcgacaa tgttcgtcaa aatggtgaca 4501 aagccctttt agaactaact gccaagtttg atggagtcgc tttgaagaca cctgtgttag 4561 aagctccttt cccagaggaa cttatgcaat tgccagataa cgttaagaga gccattgatc 4621 tctctataga taacgtcagg aaattccatg aagctcaact agcggagacg ttgcaagttg 4681 agacttgccc tggtgtagtc tgctctcgtt ttgcaagacc tattgagaaa gttggcctct 4741 atattcctgg tggaaccgca attctgcctt ccacttccct gatgctgggt gttcctgcca 4801 aagttgctgg ttgcaaagaa attgtttttg catctccacc taagaaggat ggtaccctta 4861 ccccagaagt catctacgtt gcccacaagg ttggtgctaa gtgtatcgtg ctagcaggag 4921 gcgcccaggc agtagctgct atggcttacg gaacagaaac tgttcctaag tgtgacaaaa 4981 tatttggtcc aggaaaccag ttcgttactg ctgccaagat gatggttcaa aatgacacat 5041 cagccctgtg tagtattgac atgcctgctg ggccttctga agttctagtt attgctgata 5101 aatacgctga tccagatttc gttgtctcag accttctgtc tcaagctgaa catggtattg 5161 attcccaggt gattctgttg gctgtcgata tgacagacaa ggagcttgcc agaattgaag 5221 atgctgttca caaccaagct gtgcagttgc caagggttga aattgtacgc aagtgtattg 5281 cacactctac aaccctatcg gttgcaacct acgagcaggc tttggaaatg tccaatcagt 5341 acgctcctga acacttgatc ctgcaaatcg agaatgcttc ttatgttgat caagtacaac 5401 acgctggatc tgtgtttgtt ggtgcctact ctccagagag ttgtggagat tactcctccg 5461 gtaccaacca cactttgcca acgtacggat atgcccgtca atacagcgga gttaacactg 5521 caaccttcca gaagttcatc acttcacaag acgtaactcc tgagggactg aaacatattg 5581 gccaagcagt gatggatctg gctgctgttg aaggtctaga tgctcaccgc aatgctgtta 5641 aggttcgtat ggagaaactg ggacttattt aattatttag agattttaac ttacatttag 5701 attcgataga tccgagtatc tatgattgga agtatgggaa tggtgatacc cgcattcttc 5761 agtgtcttga ggtctcctat cagattatgc ccaactaaag caaccggagg aggagatttc 5821 atggtaaatt tctctgactt ttggtcatca gtagactcga actgtgagac tatctcggtt 5881 atgacagcag aaatgtcctt cttggagaca gtaaatgaag tcccaccaat aaagaaatcc 5941 ttgttatcag gaacaaactt cttgtttcga actttttcgg tgccttgaac tataaaatgt 6001 agagtggata tgtcgggtag gaatggagcg ggcaaatgct taccttctgg accttcaaga 6061 ggtatgtagg gtttgtagat actgatgcca acttcagtga caacgttgct atttcgttca 6121 aaccattccg aatccagaga aatcaaagtt gtttgtctac tattgatcca agccagtgcg 6181 gtcttgaaac tgacaatagt gtgctcgtgt tttgaggtca tctttgtatg aataaatcta 6241 gtctttgatc taaataatct tgacgagcca aggcgataaa tacccaaatc taaaactctt 6301 ttaaaacgtt aaaaggacaa gtatgtctgc ctgtattaaa ccccaaatca gctcgtagtc 6361 tgatcctcat caacttgagg ggcactatct tgtttt //