LOCUS BBa_K864000 3639 bp DNA linear 23-May-2024 DEFINITION Low copy BioBrick standard vector ACCESSION BBa_K864000 VERSION BBa_K864000.1 FEATURES Location/Qualifiers promoter 903..908 /label=-10 promoter 3387..3392 /label=-10 promoter 881..886 /label=-35 promoter 3413..3418 /label=-35 CDS 939..1889 /label=repA misc_feature 2509..3369 /label=ampR BBa_P1002 misc_feature 867..867 /label=Mutated A>T to remove SpeI primer_bind 2433..2450 /label=Lambda Red forward primer primer_bind 18..38 /label=Lambda Red reverse primer primer_bind 3495..3514 /label=VF2 primer_bind 116..135 /label=VR RBS 3374..3378 /label=RBS misc_feature 790..809 /label=Tandem repeat misc_feature 767..788 /label=Tandem repeat misc_feature 862..880 /label=Repeat "4" misc_feature 820..841 /label=Tandem repeat misc_feature 891..912 /label=Repeat "5" misc_feature 190..2405 /label=pSC101 ori misc_feature 47..115 /label=tonB terminator misc_feature 2454..2499 /label=Lux terminator misc_feature 3515..3592 /label=T3 terminator misc_feature 136..175 /label=rrnC terminator ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaggatga tttctgctag 181 agacgcgtgg acagtaagac gggtaagcct gttgatgata ccgctgcctt actgggtgca 241 ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga 301 gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa 361 acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat 421 ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc 481 gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa 541 tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc 601 tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact 661 aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt tattctttct 721 ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg 781 aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac 841 agatacccac aactcaaagg aaaaggtcta gtaattatca ttgactagcc catctcaatt 901 ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca 961 aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt 1021 tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg 1081 tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg 1141 gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt 1201 tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat 1261 tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa 1321 aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt 1381 ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg 1441 atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc 1501 aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg aaattggtgg 1561 ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa 1621 tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa 1681 caaccattac atcagattcc tacctacata acggactaag aaaaacacta cacgatgctt 1741 taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac atgcaaagta 1801 agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga 1861 acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa 1921 gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa 1981 ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga gcttttacga 2041 gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac agatgaacag 2101 catgtaacac ctaatagaac aggtgaaacc agtaaaacaa agcaactaga acatgaaatt 2161 gaacacctga gacaacttgt tacagctcaa cagtcacaca tagacagcct gaaacaggcg 2221 atgctgctta tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc tcccgtgggg 2281 aaaaaatcat ggcaattctg gaagaaatag cgctttcagc cggcaaaccg gctgaagccg 2341 gatctgcgat tctgataaca aactagcaac accagaacag cccgtttgcg ggcagcaaaa 2401 cccgtcagct gtctcttata cactgctagc gccacacaac cacaccacac cacaaataat 2461 aaaaaagccg gattaataat ctggcttttt atattctctg tcgacttatt accaatgctt 2521 aatcagggag gcacctatct cagcgatctg gcgattacgt tcgtccatag ttgcctggct 2581 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gggctgcaat 2641 aataccgcgg gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 2701 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 2761 ttgccgggaa gcgagagtaa gaagttcgcc agttaagagt ttgcgcaacg ttgttgccat 2821 agctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 2881 ccaacgatca aggcgagtta catgatcacc catgttgtgc aaaaaagcgg taagctcctt 2941 cggtcctccg atcgttgtca gaagtaagtt ggccgccgtg ttatcactca tggttatggc 3001 agcgctgcat aattcgcgta ctgtcatgcc gtccgtaaga tgcttttctg tgactggtga 3061 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 3121 gtcaatacgg gataataccg cgccacagag cagaacttta aaagtgctca tcattgggaa 3181 acgttcttcg gggcgaaaac tctcaagaat cttaccgctg ttgaggtcca gttcgatgta 3241 acccacacgt gcacccaact gatcttcggc atcttttact ttcaccagcg tttctgggtg 3301 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 3361 aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 3421 gagcggatag atatttgaat gtatttagag ctcaaataaa gccctgagtt taaccgctcg 3481 gggctttttg cgtttgccac ctgacgtcta agaaaaggaa tattcagcaa tttgcccgtg 3541 ccgaagaaag gcccacccgt gaaggtgagc cagtgagttg attgctacgt aattagttag 3601 ttagccctta gtgactcgaa ttcgcggccg cttctagag //