##gff-version 3
##sequence-region BBa_K864000 18 3592
BBa_K864000	.	promoter	881	886	.	+	0	ID=annotation2198009;Name=-35
BBa_K864000	.	promoter	3413	3418	.	+	0	ID=annotation2198010;Name=-35
BBa_K864000	.	ribosome_entry_site	3374	3378	.	+	0	ID=annotation2198034;Name=RBS
BBa_K864000	.	promoter	903	908	.	+	0	ID=annotation2198006;Name=-10
BBa_K864000	.	sequence_feature	3515	3592	.	+	0	ID=annotation2198076;Name=T3 terminator
BBa_K864000	.	sequence_feature	767	788	.	+	0	ID=annotation2198037;Name=Tandem repeat
BBa_K864000	.	sequence_feature	2509	3369	.	+	0	ID=annotation2198012;Name=ampR BBa_P1002
BBa_K864000	.	sequence_feature	862	880	.	+	0	ID=annotation2198038;Name=Repeat "4"
BBa_K864000	.	sequence_feature	136	175	.	+	0	ID=annotation2198093;Name=rrnC terminator
BBa_K864000	.	sequence_feature	190	2405	.	+	0	ID=annotation2198057;Name=pSC101 ori
BBa_K864000	.	promoter	3387	3392	.	+	0	ID=annotation2198007;Name=-10
BBa_K864000	.	sequence_feature	891	912	.	+	0	ID=annotation2198056;Name=Repeat "5"
BBa_K864000	.	sequence_feature	2454	2499	.	+	0	ID=annotation2198075;Name=Lux terminator
BBa_K864000	.	primer_binding_site	18	38	.	+	0	ID=annotation2198031;Name=Lambda Red reverse primer
BBa_K864000	.	primer_binding_site	2433	2450	.	+	0	ID=annotation2198030;Name=Lambda Red forward primer
BBa_K864000	.	primer_binding_site	116	135	.	+	0	ID=annotation2198033;Name=VR
BBa_K864000	.	CDS	939	1889	.	+	0	ID=annotation2198011;Name=repA
BBa_K864000	.	sequence_feature	820	841	.	+	0	ID=annotation2198039;Name=Tandem repeat
BBa_K864000	.	primer_binding_site	3495	3514	.	+	0	ID=annotation2198032;Name=VF2
BBa_K864000	.	sequence_feature	47	115	.	+	0	ID=annotation2198074;Name=tonB terminator
BBa_K864000	.	sequence_feature	790	809	.	+	0	ID=annotation2198036;Name=Tandem repeat
BBa_K864000	.	sequence_alteration	867	867	.	+	0	ID=annotation2198029;Name=Mutated A>T to remove SpeI
>BBa_K864000
tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggagg
cttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctt
tcgctaggatgatttctgctagagacgcgtggacagtaagacgggtaagcctgttgatgataccgctgccttactgggt
gcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctga
acagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgta
ttatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgag
cgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgattt
gcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgac
atccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatcttttttt
attctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacaga
gggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggtct
agtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaat
tagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctg
tgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgct
cagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtgg
aaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattaga
attagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaag
tcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaa
atatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaacca
atgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccg
actgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaa
tgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgc
tttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggt
tcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttctt
atggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagg
gaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcatt
caaagctgttcaccatgaacagatcgacaatgtaacagatgaacagcatgtaacacctaatagaacaggtgaaaccagt
aaaacaaagcaactagaacatgaaattgaacacctgagacaacttgttacagctcaacagtcacacatagacagcctga
aacaggcgatgctgcttatcgaatcaaagctgccgacaacacgggagccagtgacgcctcccgtggggaaaaaatcatg
gcaattctggaagaaatagcgctttcagccggcaaaccggctgaagccggatctgcgattctgataacaaactagcaac
accagaacagcccgtttgcgggcagcaaaacccgtcagctgtctcttatacactgctagcgccacacaaccacaccaca
ccacaaataataaaaaagccggattaataatctggctttttatattctctgtcgacttattaccaatgcttaatcaggg
aggcacctatctcagcgatctggcgattacgttcgtccatagttgcctggctccccgtcgtgtagataactacgatacg
ggagggcttaccatctggccccagggctgcaataataccgcgggacccacgctcaccggctccagatttatcagcaata
aaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgcc
gggaagcgagagtaagaagttcgccagttaagagtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacg
ctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaa
aaagcggtaagctccttcggtcctccgatcgttgtcagaagtaagttggccgccgtgttatcactcatggttatggcag
cgctgcataattcgcgtactgtcatgccgtccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctg
agaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacagagcagaacttta
aaagtgctcatcattgggaaacgttcttcggggcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgt
aacccacacgtgcacccaactgatcttcggcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggca
aaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagc
atttatcagggttattgtctcatgagcggatagatatttgaatgtatttagagctcaaataaagccctgagtttaaccg
ctcggggctttttgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggccca
cccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttc
tagag