LOCUS BBa_K864001 3466 bp DNA linear 18-May-2024 DEFINITION Low copy BioBrick standard vector ACCESSION BBa_K864001 VERSION BBa_K864001.1 FEATURES Location/Qualifiers primer_bind 18..38 /label=Lambda Red reverse primer stem_loop 47..115 /label=tonB terminator primer_bind 116..135 /label=VR stem_loop 136..175 /label=rrnC terminator misc_feature 190..2405 /label= pSC101 ori misc_feature 767..788 /label=Tandem repeat misc_feature 790..809 /label=Tandem repeat misc_feature 820..841 /label=Tandem repeat misc_feature 862..880 /label=Repeat "4" misc_feature 867..867 /label=Mutated A>T to remove SpeI promoter 881..886 /label=-35 misc_feature 891..912 /label=Repeat "5" promoter 903..908 /label=-10 CDS 939..1889 /label=repA primer_bind 2433..2450 /label=Lambda Red forward primer stem_loop 3342..3419 /label=T3 terminator misc_feature 2454..2499 /label=Lux terminator CDS 2506..3168 /label=CmR BBa_P1004 promoter 3235..3261 /label=Promoter stem_loop 3251..3321 /label=P22 late terminator primer_bind 3322..3341 /label=VF2 ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaggatga tttctgctag 181 agacgcgtgg acagtaagac gggtaagcct gttgatgata ccgctgcctt actgggtgca 241 ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga 301 gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa 361 acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat 421 ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc 481 gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa 541 tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc 601 tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact 661 aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt tattctttct 721 ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg 781 aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac 841 agatacccac aactcaaagg aaaaggtcta gtaattatca ttgactagcc catctcaatt 901 ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca 961 aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt 1021 tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg 1081 tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg 1141 gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt 1201 tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat 1261 tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa 1321 aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt 1381 ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg 1441 atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc 1501 aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg aaattggtgg 1561 ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa 1621 tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa 1681 caaccattac atcagattcc tacctacata acggactaag aaaaacacta cacgatgctt 1741 taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac atgcaaagta 1801 agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga 1861 acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa 1921 gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa 1981 ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga gcttttacga 2041 gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac agatgaacag 2101 catgtaacac ctaatagaac aggtgaaacc agtaaaacaa agcaactaga acatgaaatt 2161 gaacacctga gacaacttgt tacagctcaa cagtcacaca tagacagcct gaaacaggcg 2221 atgctgctta tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc tcccgtgggg 2281 aaaaaatcat ggcaattctg gaagaaatag cgctttcagc cggcaaaccg gctgaagccg 2341 gatctgcgat tctgataaca aactagcaac accagaacag cccgtttgcg ggcagcaaaa 2401 cccgtcagct gtctcttata cactgctagc gccacacaac cacaccacac cacaaataat 2461 aaaaaagccg gattaataat ctggcttttt atattctctg tcgacttatt acgccccgcc 2521 ctgccactca tcacagtact gttgtaattc attaagcatg cggccgacat ggaagccatc 2581 acaaacggca tgatgaacct ggatcgccag cggcatcagc accttgtcgc cttgcgtata 2641 atatttgccc atcgtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc 2701 aaaactggtg aaactcaccc agggattggc tgagacaaaa aacatattct caataaaccc 2761 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtggag 2821 aaactgccgg aaatcgtcgt ggtattcact ccagagggac gaaaacgttt cagtttgctc 2881 atggaaaacg gtgtaacaag ggtgaacact atcccagatc accagctcac cgtctttcat 2941 ggccatacga aactccgggt gagcgttcat caggcgggca agaatgtgaa taaaggccgg 3001 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac 3061 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg 3121 ccattgggat atatcaacgg tggtatatcc cgtgattttt ttctccattt tagcttcctt 3181 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg 3241 gtgaaagttg gaacctctta cgtgcccgat caacgagctc aaataaagcc ctgagtttaa 3301 ccgctcgggg ctttttgcgt ttgccacctg acgtctaaga aaaggaatat tcagcaattt 3361 gcccgtgccg aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gctacgtaat 3421 tagttagtta gcccttagtg actcgaattc gcggccgctt ctagag //