##gff-version 3 ##sequence-region BBa_K864001 18 3419 BBa_K864001 . promoter 3235 3261 . + 0 ID=annotation2198622;Name=Promoter BBa_K864001 . sequence_feature 790 809 . + 0 ID=annotation2198576;Name=Tandem repeat BBa_K864001 . promoter 881 886 . + 0 ID=annotation2198584;Name=-35 BBa_K864001 . sequence_feature 862 880 . + 0 ID=annotation2198580;Name=Repeat "4" BBa_K864001 . stem_loop 47 115 . + 0 ID=annotation2198561;Name=tonB terminator BBa_K864001 . sequence_feature 767 788 . + 0 ID=annotation2198574;Name=Tandem repeat BBa_K864001 . primer_binding_site 18 38 . + 0 ID=annotation2198340;Name=Lambda Red reverse primer BBa_K864001 . primer_binding_site 3322 3341 . + 0 ID=annotation2198629;Name=VF2 BBa_K864001 . CDS 939 1889 . + 0 ID=annotation2198605;Name=repA BBa_K864001 . sequence_feature 820 841 . + 0 ID=annotation2198578;Name=Tandem repeat BBa_K864001 . CDS 2506 3168 . + 0 ID=annotation2198620;Name=CmR BBa_P1004 BBa_K864001 . promoter 903 908 . + 0 ID=annotation2198603;Name=-10 BBa_K864001 . stem_loop 136 175 . + 0 ID=annotation2198566;Name=rrnC terminator BBa_K864001 . primer_binding_site 116 135 . + 0 ID=annotation2198565;Name=VR BBa_K864001 . primer_binding_site 2433 2450 . + 0 ID=annotation2198606;Name=Lambda Red forward primer BBa_K864001 . sequence_feature 190 2405 . + 0 ID=annotation2198568;Name= pSC101 ori BBa_K864001 . stem_loop 3251 3321 . + 0 ID=annotation2198628;Name=P22 late terminator BBa_K864001 . stem_loop 3342 3419 . + 0 ID=annotation2198614;Name=T3 terminator BBa_K864001 . sequence_feature 2454 2499 . + 0 ID=annotation2198616;Name=Lux terminator BBa_K864001 . sequence_feature 891 912 . + 0 ID=annotation2198585;Name=Repeat "5" BBa_K864001 . sequence_feature 867 867 . + 0 ID=annotation2198582;Name=Mutated A>T to remove SpeI >BBa_K864001 tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggagg cttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctt tcgctaggatgatttctgctagagacgcgtggacagtaagacgggtaagcctgttgatgataccgctgccttactgggt gcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctga acagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgta ttatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgag cgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgattt gcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgac atccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatcttttttt attctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacaga gggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggtct agtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaat tagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctg tgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgct cagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtgg aaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattaga attagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaag tcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaa atatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaacca atgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccg actgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaa tgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgc tttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggt tcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttctt atggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagg gaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcatt caaagctgttcaccatgaacagatcgacaatgtaacagatgaacagcatgtaacacctaatagaacaggtgaaaccagt aaaacaaagcaactagaacatgaaattgaacacctgagacaacttgttacagctcaacagtcacacatagacagcctga aacaggcgatgctgcttatcgaatcaaagctgccgacaacacgggagccagtgacgcctcccgtggggaaaaaatcatg gcaattctggaagaaatagcgctttcagccggcaaaccggctgaagccggatctgcgattctgataacaaactagcaac accagaacagcccgtttgcgggcagcaaaacccgtcagctgtctcttatacactgctagcgccacacaaccacaccaca ccacaaataataaaaaagccggattaataatctggctttttatattctctgtcgacttattacgccccgccctgccact catcacagtactgttgtaattcattaagcatgcggccgacatggaagccatcacaaacggcatgatgaacctggatcgc cagcggcatcagcaccttgtcgccttgcgtataatatttgcccatcgtgaaaacgggggcgaagaagttgtccatattg gccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacaaaaaacatattctcaataaaccctttag ggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtggagaaactgccggaaatcgtcgtggta ttcactccagagggacgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccagatcacc agctcaccgtctttcatggccatacgaaactccgggtgagcgttcatcaggcgggcaagaatgtgaataaaggccggat aaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattg agcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatcccgtgattttt ttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattat ggtgaaagttggaacctcttacgtgcccgatcaacgagctcaaataaagccctgagtttaaccgctcggggctttttgc gtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagc cagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagag