LOCUS BBa_K864002 3663 bp DNA linear 22-Nov-2024 DEFINITION Low copy BioBrick standard vector ACCESSION BBa_K864002 VERSION BBa_K864002.1 FEATURES Location/Qualifiers primer_bind 18..38 /label=Lambda Red reverse primer stem_loop 47..115 /label=TonB terminator primer_bind 116..135 /label=VR stem_loop 136..175 /label=rrnC terminator misc_feature 190..2405 /label=pSC101 ori BBa_K864050 primer_bind 2433..2450 /label=Lambda Red forward primer stem_loop 2454..2499 /label=Lux terminator misc_feature 2506..3224 /label=KanR BBa_P1003 stem_loop 3478..3518 /label=P22 late terminator primer_bind 3519..3538 /label=VF2 stem_loop 3539..3616 /label=T3 terminator ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaggatga tttctgctag 181 agacgcgtgg acagtaagac gggtaagcct gttgatgata ccgctgcctt actgggtgca 241 ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga 301 gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa 361 acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat 421 ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc 481 gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa 541 tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc 601 tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact 661 aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt tattctttct 721 ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg 781 aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac 841 agatacccac aactcaaagg aaaaggtcta gtaattatca ttgactagcc catctcaatt 901 ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca 961 aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt 1021 tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg 1081 tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg 1141 gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt 1201 tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat 1261 tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa 1321 aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt 1381 ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg 1441 atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc 1501 aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg aaattggtgg 1561 ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa 1621 tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa 1681 caaccattac atcagattcc tacctacata acggactaag aaaaacacta cacgatgctt 1741 taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac atgcaaagta 1801 agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga 1861 acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa 1921 gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa 1981 ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga gcttttacga 2041 gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac agatgaacag 2101 catgtaacac ctaatagaac aggtgaaacc agtaaaacaa agcaactaga acatgaaatt 2161 gaacacctga gacaacttgt tacagctcaa cagtcacaca tagacagcct gaaacaggcg 2221 atgctgctta tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc tcccgtgggg 2281 aaaaaatcat ggcaattctg gaagaaatag cgctttcagc cggcaaaccg gctgaagccg 2341 gatctgcgat tctgataaca aactagcaac accagaacag cccgtttgcg ggcagcaaaa 2401 cccgtcagct gtctcttata cactgctagc gccacacaac cacaccacac cacaaataat 2461 aaaaaagccg gattaataat ctggcttttt atattctctg tcgacttatt agaaaaactc 2521 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg 2581 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccaaa gaatggcaag 2641 gtcctggtaa cggtctgcga ttccgacccg tccaacatca atacaaccta ttaatttccc 2701 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga 2761 gaatggcaag agcttgtgca tttctttcca gacttgttca acaggccagc cattacgctc 2821 gtcatcaaaa tcactcgcat caaccaaacc gttattcatg cgtgattgcg cctgagcaag 2881 acgaaataca cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gtaaccggcg 2941 caggaacacg gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac 3001 ctggaaggct gttttcccag gaatcgcggt ggtgagtaac cacgcatcat caggagtacg 3061 gataaaatgc ttgatggtcg ggagaggcat aaactccgtc agccagttga gacggaccat 3121 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc 3181 atcgggcttc ccatacaagc gatagattgt cgcacctgat tgcccgacat tatcgcgagc 3241 ccatttatac ccatataaat cagcgtccat gttggagttt aagcgcggac gggagcaaga 3301 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag 3361 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga 3421 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agagctcaaa 3481 taaagccctg agtttaaccg ctcggggctt tttgcgtttg ccacctgacg tctaagaaaa 3541 ggaatattca gcaatttgcc cgtgccgaag aaaggcccac ccgtgaaggt gagccagtga 3601 gttgattgct acgtaattag ttagttagcc cttagtgact cgaattcgcg gccgcttcta 3661 gag //