LOCUS BBa_K864003 3793 bp DNA linear 22-Nov-2024 DEFINITION Low copy BioBrick standard vector ACCESSION BBa_K864003 VERSION BBa_K864003.1 FEATURES Location/Qualifiers primer_bind 18..38 /label=Lambda Red reverse primer stem_loop 47..115 /label=TonB terminator primer_bind 116..135 /label=VR stem_loop 136..175 /label=rnC terminator misc_feature 190..2405 /label=pSC101 ori BBa_K864050 primer_bind 2433..2450 /label=Lambda Red forward primer stem_loop 2454..2499 /label=Lux terminator CDS 2510..3508 /label=spcR promoter 3575..3601 /label=Promoter stem_loop 3608..3648 /label=P22 late terminator primer_bind 3649..3668 /label=VF2 stem_loop 3669..3746 /label=T3 terminator ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaggatga tttctgctag 181 agacgcgtgg acagtaagac gggtaagcct gttgatgata ccgctgcctt actgggtgca 241 ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga 301 gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa 361 acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat 421 ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc 481 gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa 541 tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc 601 tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact 661 aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt tattctttct 721 ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg 781 aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac 841 agatacccac aactcaaagg aaaaggtcta gtaattatca ttgactagcc catctcaatt 901 ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca 961 aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt 1021 tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg 1081 tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg 1141 gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt 1201 tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat 1261 tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa 1321 aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt 1381 ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg 1441 atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc 1501 aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg aaattggtgg 1561 ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa 1621 tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa 1681 caaccattac atcagattcc tacctacata acggactaag aaaaacacta cacgatgctt 1741 taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac atgcaaagta 1801 agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga 1861 acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa 1921 gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa 1981 ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga gcttttacga 2041 gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac agatgaacag 2101 catgtaacac ctaatagaac aggtgaaacc agtaaaacaa agcaactaga acatgaaatt 2161 gaacacctga gacaacttgt tacagctcaa cagtcacaca tagacagcct gaaacaggcg 2221 atgctgctta tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc tcccgtgggg 2281 aaaaaatcat ggcaattctg gaagaaatag cgctttcagc cggcaaaccg gctgaagccg 2341 gatctgcgat tctgataaca aactagcaac accagaacag cccgtttgcg ggcagcaaaa 2401 cccgtcagct gtctcttata cactgctagc gccacacaac cacaccacac cacaaataat 2461 aaaaaagccg gattaataat ctggcttttt atattctctg tcgacttatt accttggtga 2521 tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat 2581 cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg 2641 gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg 2701 cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg 2761 gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa 2821 ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt 2881 ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa 2941 tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa 3001 tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag 3061 gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc 3121 ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg 3181 cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa 3241 ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcatg atgtttaact 3301 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc 3361 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa 3421 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca 3481 aggttctgga ccagttgcgt aagcgcattt tagcttcctt agctcctgaa aatctcgata 3541 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta 3601 cgagctcaaa taaagccctg agtttaaccg ctcggggctt tttgcgtttg ccacctgacg 3661 tctaagaaaa ggaatattca gcaatttgcc cgtgccgaag aaaggcccac ccgtgaaggt 3721 gagccagtga gttgattgct acgtaattag ttagttagcc cttagtgact cgaattcgcg 3781 gccgcttcta gag //