LOCUS BBa_K864004 3708 bp DNA linear 23-May-2024 DEFINITION Low copy BioBrick standard vector ACCESSION BBa_K864004 VERSION BBa_K864004.1 FEATURES Location/Qualifiers primer_bind 18..38 /label=Lambda Red reverse primer stem_loop 47..115 /label=TonB terminator primer_bind 116..135 /label=VR stem_loop 136..175 /label=rrnC terminator misc_feature 190..2405 /label=pSC101 ori BBa_K864050 primer_bind 2433..2450 /label=Lambda Red forward primer stem_loop 2454..2499 /label=Lux terminator stem_loop 3523..3563 /label=P22 late terminator primer_bind 3564..3583 /label=VF2 stem_loop 3584..3661 /label=T3 terminator CDS 2543..3403 /label=AmpR P1002 RBS 3408..3412 /label=RBS promoter 3421..3426 /label=-10 promoter 3447..3452 /label=-35 misc_feature 2506..2539 /label=FRT site misc_feature 3483..3516 /label=FRT site ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaggatga tttctgctag 181 agacgcgtgg acagtaagac gggtaagcct gttgatgata ccgctgcctt actgggtgca 241 ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga 301 gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa 361 acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat 421 ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc 481 gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa 541 tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc 601 tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact 661 aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt tattctttct 721 ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg 781 aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac 841 agatacccac aactcaaagg aaaaggtcta gtaattatca ttgactagcc catctcaatt 901 ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca 961 aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt 1021 tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg 1081 tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg 1141 gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt 1201 tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat 1261 tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa 1321 aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt 1381 ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg 1441 atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc 1501 aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg aaattggtgg 1561 ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa 1621 tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa 1681 caaccattac atcagattcc tacctacata acggactaag aaaaacacta cacgatgctt 1741 taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac atgcaaagta 1801 agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga 1861 acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa 1921 gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa 1981 ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga gcttttacga 2041 gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac agatgaacag 2101 catgtaacac ctaatagaac aggtgaaacc agtaaaacaa agcaactaga acatgaaatt 2161 gaacacctga gacaacttgt tacagctcaa cagtcacaca tagacagcct gaaacaggcg 2221 atgctgctta tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc tcccgtgggg 2281 aaaaaatcat ggcaattctg gaagaaatag cgctttcagc cggcaaaccg gctgaagccg 2341 gatctgcgat tctgataaca aactagcaac accagaacag cccgtttgcg ggcagcaaaa 2401 cccgtcagct gtctcttata cactgctagc gccacacaac cacaccacac cacaaataat 2461 aaaaaagccg gattaataat ctggcttttt atattctctg tcgacgaagt tcctattctc 2521 taaaaagtat aggaacttct tattaccaat gcttaatcag ggaggcacct atctcagcga 2581 tctggcgatt acgttcgtcc atagttgcct ggctccccgt cgtgtagata actacgatac 2641 gggagggctt accatctggc cccagggctg caataatacc gcgggaccca cgctcaccgg 2701 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 2761 caactttatc cgcctccatc cagtctatta attgttgccg ggaagcgaga gtaagaagtt 2821 cgccagttaa gagtttgcgc aacgttgttg ccatagctac aggcatcgtg gtgtcacgct 2881 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 2941 cacccatgtt gtgcaaaaaa gcggtaagct ccttcggtcc tccgatcgtt gtcagaagta 3001 agttggccgc cgtgttatca ctcatggtta tggcagcgct gcataattcg cgtactgtca 3061 tgccgtccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 3121 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 3181 agagcagaac tttaaaagtg ctcatcattg ggaaacgttc ttcggggcga aaactctcaa 3241 gaatcttacc gctgttgagg tccagttcga tgtaacccac acgtgcaccc aactgatctt 3301 cggcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 3361 caaaaaaggg aataagggcg acacggaaat gttgaatact catattcttc ctttttcaat 3421 attattgaag catttatcag ggttattgtc tcatgagcgg atagatattt gaatgtattt 3481 aggaagttcc tattctctaa aaagtatagg aacttcgagc tcaaataaag ccctgagttt 3541 aaccgctcgg ggctttttgc gtttgccacc tgacgtctaa gaaaaggaat attcagcaat 3601 ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc agtgagttga ttgctacgta 3661 attagttagt tagcccttag tgactcgaat tcgcggccgc ttctagag //