##gff-version 3
##sequence-region BBa_K864004 18 3661
BBa_K864004	.	sequence_feature	190	2405	.	+	0	ID=annotation2198869;Name=pSC101 ori BBa_K864050
BBa_K864004	.	ribosome_entry_site	3408	3412	.	+	0	ID=annotation2198876;Name=RBS
BBa_K864004	.	stem_loop	136	175	.	+	0	ID=annotation2198868;Name=rrnC terminator
BBa_K864004	.	promoter	3447	3452	.	+	0	ID=annotation2198878;Name=-35
BBa_K864004	.	sequence_feature	2506	2539	.	+	0	ID=annotation2198879;Name=FRT site
BBa_K864004	.	primer_binding_site	18	38	.	+	0	ID=annotation2198865;Name=Lambda Red reverse primer
BBa_K864004	.	primer_binding_site	2433	2450	.	+	0	ID=annotation2198870;Name=Lambda Red forward primer
BBa_K864004	.	CDS	2543	3403	.	+	0	ID=annotation2198875;Name=AmpR P1002
BBa_K864004	.	promoter	3421	3426	.	+	0	ID=annotation2198877;Name=-10
BBa_K864004	.	sequence_feature	3483	3516	.	+	0	ID=annotation2198880;Name=FRT site
BBa_K864004	.	stem_loop	2454	2499	.	+	0	ID=annotation2198871;Name=Lux terminator
BBa_K864004	.	primer_binding_site	3564	3583	.	+	0	ID=annotation2198873;Name=VF2
BBa_K864004	.	stem_loop	47	115	.	+	0	ID=annotation2198866;Name=TonB terminator
BBa_K864004	.	stem_loop	3584	3661	.	+	0	ID=annotation2198874;Name=T3 terminator
BBa_K864004	.	primer_binding_site	116	135	.	+	0	ID=annotation2198867;Name=VR
BBa_K864004	.	stem_loop	3523	3563	.	+	0	ID=annotation2198872;Name=P22 late terminator
>BBa_K864004
tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggagg
cttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctt
tcgctaggatgatttctgctagagacgcgtggacagtaagacgggtaagcctgttgatgataccgctgccttactgggt
gcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctga
acagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgta
ttatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgag
cgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgattt
gcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgac
atccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatcttttttt
attctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacaga
gggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggtct
agtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaat
tagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctg
tgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgct
cagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtgg
aaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattaga
attagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaag
tcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaa
atatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaacca
atgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccg
actgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaa
tgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgc
tttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggt
tcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttctt
atggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagg
gaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcatt
caaagctgttcaccatgaacagatcgacaatgtaacagatgaacagcatgtaacacctaatagaacaggtgaaaccagt
aaaacaaagcaactagaacatgaaattgaacacctgagacaacttgttacagctcaacagtcacacatagacagcctga
aacaggcgatgctgcttatcgaatcaaagctgccgacaacacgggagccagtgacgcctcccgtggggaaaaaatcatg
gcaattctggaagaaatagcgctttcagccggcaaaccggctgaagccggatctgcgattctgataacaaactagcaac
accagaacagcccgtttgcgggcagcaaaacccgtcagctgtctcttatacactgctagcgccacacaaccacaccaca
ccacaaataataaaaaagccggattaataatctggctttttatattctctgtcgacgaagttcctattctctaaaaagt
ataggaacttcttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgttcgtccatagttg
cctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaataataccgcggga
cccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaact
ttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaagagtttgcgcaacg
ttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatc
aaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgttgtcagaagtaag
ttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtccgtaagatgctttt
ctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaat
acgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggggcgaaaactctca
agaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggcatcttttactttca
ccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaat
actcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatagatatttgaatgt
atttaggaagttcctattctctaaaaagtataggaacttcgagctcaaataaagccctgagtttaaccgctcggggctt
tttgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaagg
tgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagag