LOCUS BBa_K864005 3534 bp DNA linear 22-Nov-2024 DEFINITION Low copy BioBrick standard vector ACCESSION BBa_K864005 VERSION BBa_K864005.1 FEATURES Location/Qualifiers primer_bind 18..38 /label=Lambda Red reverse primer stem_loop 47..115 /label=TonB terminator primer_bind 116..135 /label=VR stem_loop 136..175 /label=rrnC terminator misc_feature 190..2405 /label=pSC101 ori BBa_K864050 primer_bind 2433..2450 /label=Lambda Red forward primer stem_loop 2454..2499 /label=Lux terminator ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaggatga tttctgctag 181 agacgcgtgg acagtaagac gggtaagcct gttgatgata ccgctgcctt actgggtgca 241 ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga 301 gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa 361 acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat 421 ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc 481 gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa 541 tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc 601 tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact 661 aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt tattctttct 721 ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg 781 aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac 841 agatacccac aactcaaagg aaaaggtcta gtaattatca ttgactagcc catctcaatt 901 ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca 961 aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt 1021 tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg 1081 tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg 1141 gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt 1201 tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat 1261 tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa 1321 aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt 1381 ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg 1441 atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc 1501 aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg aaattggtgg 1561 ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa 1621 tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa 1681 caaccattac atcagattcc tacctacata acggactaag aaaaacacta cacgatgctt 1741 taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac atgcaaagta 1801 agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga 1861 acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa 1921 gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa 1981 ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga gcttttacga 2041 gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac agatgaacag 2101 catgtaacac ctaatagaac aggtgaaacc agtaaaacaa agcaactaga acatgaaatt 2161 gaacacctga gacaacttgt tacagctcaa cagtcacaca tagacagcct gaaacaggcg 2221 atgctgctta tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc tcccgtgggg 2281 aaaaaatcat ggcaattctg gaagaaatag cgctttcagc cggcaaaccg gctgaagccg 2341 gatctgcgat tctgataaca aactagcaac accagaacag cccgtttgcg ggcagcaaaa 2401 cccgtcagct gtctcttata cactgctagc gccacacaac cacaccacac cacaaataat 2461 aaaaaagccg gattaataat ctggcttttt atattctctg tcgacgaagt tcctattctc 2521 taaaaagtat aggaacttct tattacgccc cgccctgcca ctcatcacag tactgttgta 2581 attcattaag catgcggccg acatggaagc catcacaaac ggcatgatga acctggatcg 2641 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatcgtg aaaacggggg 2701 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat 2761 tggctgagac aaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac 2821 cgtaacacgc cacatcttgc gaatatatgt ggagaaactg ccggaaatcg tcgtggtatt 2881 cactccagag ggacgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa 2941 cactatccca gatcaccagc tcaccgtctt tcatggccat acgaaactcc gggtgagcgt 3001 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta 3061 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa 3121 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat 3181 atcccgtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa 3241 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc 3301 cgatcaacga agttcctatt ctctaaaaag tataggaact tcgagctcaa ataaagccct 3361 gagtttaacc gctcggggct ttttgcgttt gccacctgac gtctaagaaa aggaatattc 3421 agcaatttgc ccgtgccgaa gaaaggccca cccgtgaagg tgagccagtg agttgattgc 3481 tacgtaatta gttagttagc ccttagtgac tcgaattcgc ggccgcttct agag //