LOCUS BBa_K864009 4646 bp DNA linear 22-Dec-2024 DEFINITION Low copy BioBrick standard vector with LacI ACCESSION BBa_K864009 VERSION BBa_K864009.1 FEATURES Location/Qualifiers misc_feature 190..2405 /label=pSC101 ori promoter 4415..4441 /label=Promoter promoter 2439..2494 /label=PlacIq promoter CDS 2521..3603 /label=lacI CDS 3686..4348 /label=cmR RBS 2509..2514 /label=RBS misc_feature 881..886 /label=-35 misc_feature 2441..2446 /label=-35 misc_feature 3634..3679 /label=Lux terminator misc_feature 4461..4501 /label=P22 late terminator misc_feature 4522..4599 /label=T3 terminator misc_feature 47..115 /label=tonB terminator misc_feature 136..175 /label=rrnC misc_feature 903..908 /label=-10 misc_feature 2464..2469 /label=-10 ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaggatga tttctgctag 181 agacgcgtgg acagtaagac gggtaagcct gttgatgata ccgctgcctt actgggtgca 241 ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga 301 gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa 361 acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat 421 ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc 481 gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa 541 tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc 601 tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact 661 aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt tattctttct 721 ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg 781 aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac 841 agatacccac aactcaaagg aaaaggtcta gtaattatca ttgactagcc catctcaatt 901 ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca 961 aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt 1021 tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg 1081 tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg 1141 gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt 1201 tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat 1261 tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa 1321 aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt 1381 ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg 1441 atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc 1501 aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg aaattggtgg 1561 ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa 1621 tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa 1681 caaccattac atcagattcc tacctacata acggactaag aaaaacacta cacgatgctt 1741 taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac atgcaaagta 1801 agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga 1861 acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa 1921 gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa 1981 ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga gcttttacga 2041 gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac agatgaacag 2101 catgtaacac ctaatagaac aggtgaaacc agtaaaacaa agcaactaga acatgaaatt 2161 gaacacctga gacaacttgt tacagctcaa cagtcacaca tagacagcct gaaacaggcg 2221 atgctgctta tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc tcccgtgggg 2281 aaaaaatcat ggcaattctg gaagaaatag cgctttcagc cggcaaaccg gctgaagccg 2341 gatctgcgat tctgataaca aactagcaac accagaacag cccgtttgcg ggcagcaaaa 2401 cccgtcagct gtctcttata cactgctagc tgcatttacg ttgacaccat cgaatggtgc 2461 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggaggtgaat 2521 atgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt 2581 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg 2641 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag 2701 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc 2761 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa 2821 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt 2881 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc 2941 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt 3001 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag 3061 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc 3121 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg 3181 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact 3241 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc 3301 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca 3361 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc 3421 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc 3481 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc 3541 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 3601 taataagtcg agcacacaac cacaccacac cacaaataat aaaaaagccg gattaataat 3661 ctggcttttt atattctctg tcgacttatt acgccccgcc ctgccactca tcacagtact 3721 gttgtaattc attaagcatg cggccgacat ggaagccatc acaaacggca tgatgaacct 3781 ggatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atcgtgaaaa 3841 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc 3901 agggattggc tgagacaaaa aacatattct caataaaccc tttagggaaa taggccaggt 3961 tttcaccgta acacgccaca tcttgcgaat atatgtggag aaactgccgg aaatcgtcgt 4021 ggtattcact ccagagggac gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag 4081 ggtgaacact atcccagatc accagctcac cgtctttcat ggccatacga aactccgggt 4141 gagcgttcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt 4201 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt 4261 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg 4321 tggtatatcc cgtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata 4381 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta 4441 cgtgcccgat caacgagctc aaataaagcc ctgagtttaa ccgctcgggg ctttttgcgt 4501 ttgccacctg acgtctaaga aaaggaatat tcagcaattt gcccgtgccg aagaaaggcc 4561 cacccgtgaa ggtgagccag tgagttgatt gctacgtaat tagttagtta gcccttagtg 4621 actcgaattc gcggccgctt ctagag //