LOCUS BBa_K864022 3732 bp DNA linear 25-Nov-2024 DEFINITION Low copy BioBrick temperature sensitive standard vector ACCESSION BBa_K864022 VERSION BBa_K864022.1 FEATURES Location/Qualifiers ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaggatga tttctgctag 181 agacgcgtgg acagtaagac gggtaagcct gttgatgata ccgctgcctt actgggtgca 241 ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga 301 gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa 361 acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat 421 ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc 481 gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa 541 tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc 601 tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact 661 aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt tattctttct 721 ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg 781 aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac 841 agatacccac aactcaaagg aaaaggtcta gtaattatca ttgactagcc catctcaatt 901 ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca 961 aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt 1021 tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg 1081 tatcgttcac ttataaccaa tacgttcaga tgatgaacat cagtagggaa aatgcttatg 1141 gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt 1201 tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat 1261 tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa 1321 aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt 1381 ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg 1441 atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc 1501 aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg aaattggtgg 1561 ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa 1621 tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa 1681 caaccattac atcagattcc tacctacata acggactaag aaaaacacta cacgatgctt 1741 taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac atgcaaagta 1801 agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga 1861 acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa 1921 gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa 1981 ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga gcttttacga 2041 gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac agatgaacag 2101 catgtaacac ctaatagaac aggtgaaacc agtaaaacaa agcaactaga acatgaaatt 2161 gaacacctga gacaacttgt tacagctcaa cagtcacaca tagacagcct gaaacaggcg 2221 atgctgctta tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc tcccgtgggg 2281 aaaaaatcat ggcaattctg gaagaaatag cgctttcagc cggcaaaccg gctgaagccg 2341 gatctgcgat tctgataaca aactagcaac accagaacag cccgtttgcg ggcagcaaaa 2401 cccgtcagct gtctcttata cactgctagc gccacacaac cacaccacac cacaaataat 2461 aaaaaagccg gattaataat ctggcttttt atattctctg tcgacgaagt tcctattctc 2521 taaaaagtat aggaacttct tattagaaaa actcatcgag catcaaatga aactgcaatt 2581 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag 2641 aaaactcacc gaggcagttc caaagaatgg caaggtcctg gtaacggtct gcgattccga 2701 cccgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg 2761 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caagagcttg tgcatttctt 2821 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca 2881 aaccgttatt catgcgtgat tgcgcctgag caagacgaaa tacacgatcg ctgttaaaag 2941 gacaattaca aacaggaatc gaatgtaacc ggcgcaggaa cacggccagc gcatcaacaa 3001 tattttcacc tgaatcagga tattcttcta atacctggaa ggctgttttc ccaggaatcg 3061 cggtggtgag taaccacgca tcatcaggag tacggataaa atgcttgatg gtcgggagag 3121 gcataaactc cgtcagccag ttgagacgga ccatctcatc tgtaacatca ttggcaacgc 3181 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aagcgataga 3241 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcgt 3301 ccatgttgga gtttaagcgc ggacgggagc aagacgtttc ccgttgaata tggctcataa 3361 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt 3421 tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt tgaataaatc 3481 gaacttttgc tgagttgaag gatcaggaag ttcctattct ctaaaaagta taggaacttc 3541 gagctcaaat aaagccctga gtttaaccgc tcggggcttt ttgcgtttgc cacctgacgt 3601 ctaagaaaag gaatattcag caatttgccc gtgccgaaga aaggcccacc cgtgaaggtg 3661 agccagtgag ttgattgcta cgtaattagt tagttagccc ttagtgactc gaattcgcgg 3721 ccgcttctag ag //