LOCUS BBa_K864050 2216 bp DNA linear 31-Oct-2024 DEFINITION pSC101 low copy origin of replication ACCESSION BBa_K864050 VERSION BBa_K864050.1 FEATURES Location/Qualifiers misc_feature 1..368 /label=par region misc_feature 1..2216 /label=pSC101 ori misc_feature 190..204 /label=Repeat structure misc_feature 232..247 /label=Repeat structure misc_feature 499..581 /label=AT rich region misc_feature 578..599 /label=Tandem repeat misc_feature 582..589 /label=Repeated sequence misc_feature 601..620 /label=Tandem repeat misc_feature 603..610 /label=Repeated sequence misc_feature 631..652 /label=Tandem repeat misc_feature 635..642 /label=Repeated sequence misc_feature 673..691 /label=Repeat "4" misc_feature 676..683 /label=Repeated sequence misc_feature 678..678 /label=A>T mutation to remove SpeI stem_loop 683..761 /label=Hairpin structure promoter 692..697 /label=-35 signal misc_feature 702..723 /label=Repeat "5" misc_feature 712..719 /label=Repeated sequence CDS 750..1700 /label=repA stem_loop 1608..1682 /label=Hairpin structure misc_feature 2141..2143 /label=Ins(GGC) ORIGIN 1 gacagtaaga cgggtaagcc tgttgatgat accgctgcct tactgggtgc attagccagt 61 ctgaatgacc tgtcacggga taatccgaag tggtcagact ggaaaatcag agggcaggaa 121 ctgctgaaca gcaaaaagtc agatagcacc acatagcaga cccgccataa aacgccctga 181 gaagcccgtg acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag 241 gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat 301 gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg 361 atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta 421 gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg 481 agttatacac agggctggga tctattcttt ttatcttttt ttattctttc tttattctat 541 aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag 601 agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta cagataccca 661 caactcaaag gaaaaggtct agtaattatc attgactagc ccatctcaat tggtatagtg 721 attaaaatca cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg 781 aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg 841 tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca 901 cttataacca atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag 961 ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag 1021 gctttgagat tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag 1081 tttttagtga agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc 1141 tggaacatgt taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa 1201 aagaactaac acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta 1261 agttcatgtt aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt 1321 tgaaaccaat aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc 1381 gaggccgccc gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg 1441 taaccgaact tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta 1501 catcagattc ctacctacat aacggactaa gaaaaacact acacgatgct ttaactgcaa 1561 aaattcagct caccagtttt gaggcaaaat ttttgagtga catgcaaagt aagtatgatc 1621 tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg 1681 ctaaatacgg aaggatctga ggttcttatg gctcttgtat ctatcagtga agcatcaaga 1741 ctaacaaaca aaagtagaac aactgttcac cgttacatat caaagggaaa actgtccata 1801 tgcacagatg aaaacggtgt aaaaaagata gatacatcag agcttttacg agtttttggt 1861 gcattcaaag ctgttcacca tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca 1921 cctaatagaa caggtgaaac cagtaaaaca aagcaactag aacatgaaat tgaacacctg 1981 agacaacttg ttacagctca acagtcacac atagacagcc tgaaacaggc gatgctgctt 2041 atcgaatcaa agctgccgac aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca 2101 tggcaattct ggaagaaata gcgctttcag ccggcaaacc ggctgaagcc ggatctgcga 2161 ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgt //