LOCUS       BBa_K864051             2216 bp    DNA     linear       31-Oct-2024
DEFINITION  pSC101ts temperature sensitive low copy origin of replication
ACCESSION   BBa_K864051
VERSION     BBa_K864051.1  
FEATURES             Location/Qualifiers
     misc_feature    915..917
                     /label=A56V ts mutation
     misc_feature    1..2216
                     /label=pSC101ts ori
     misc_feature    1..368
                     /label=par region
     misc_feature    190..204
                     /label=Repeat structure
     misc_feature    232..247
                     /label=Repeat structure
     misc_feature    499..581
                     /label=AT rich region
     misc_feature    578..599
                     /label=Tandem repeat
     misc_feature    582..589
                     /label=Repeated sequence
     misc_feature    601..620
                     /label=Tandem repeat
     misc_feature    603..610
                     /label=Repeated sequence
     misc_feature    631..652
                     /label=Tandem repeat
     misc_feature    635..642
                     /label=Repeated sequence
     misc_feature    671..693
                     /label=Repeat "4"
     misc_feature    676..683
                     /label=Repeated sequence
     misc_feature    678..678
                     /label=A>T mutation to remove SpeI
     stem_loop       683..761
                     /label=Hairpin structure
     promoter        692..697
                     /label=-35 signal
     CDS             750..1700
                     /label=repA
     misc_feature    702..723
                     /label=Repeat "5"
     misc_feature    712..719
                     /label=Repeated sequence
     stem_loop       1608..1682
                     /label=Hairpin structure
     misc_feature    2141..2143
                     /label=Ins(GGC)
ORIGIN
        1 gacagtaaga cgggtaagcc tgttgatgat accgctgcct tactgggtgc attagccagt
       61 ctgaatgacc tgtcacggga taatccgaag tggtcagact ggaaaatcag agggcaggaa
      121 ctgctgaaca gcaaaaagtc agatagcacc acatagcaga cccgccataa aacgccctga
      181 gaagcccgtg acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag
      241 gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat
      301 gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg
      361 atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta
      421 gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg
      481 agttatacac agggctggga tctattcttt ttatcttttt ttattctttc tttattctat
      541 aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag
      601 agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta cagataccca
      661 caactcaaag gaaaaggtct agtaattatc attgactagc ccatctcaat tggtatagtg
      721 attaaaatca cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg
      781 aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg
      841 tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca
      901 cttataacca atacgttcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag
      961 ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag
     1021 gctttgagat tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag
     1081 tttttagtga agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc
     1141 tggaacatgt taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa
     1201 aagaactaac acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta
     1261 agttcatgtt aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt
     1321 tgaaaccaat aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc
     1381 gaggccgccc gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg
     1441 taaccgaact tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta
     1501 catcagattc ctacctacat aacggactaa gaaaaacact acacgatgct ttaactgcaa
     1561 aaattcagct caccagtttt gaggcaaaat ttttgagtga catgcaaagt aagtatgatc
     1621 tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg
     1681 ctaaatacgg aaggatctga ggttcttatg gctcttgtat ctatcagtga agcatcaaga
     1741 ctaacaaaca aaagtagaac aactgttcac cgttacatat caaagggaaa actgtccata
     1801 tgcacagatg aaaacggtgt aaaaaagata gatacatcag agcttttacg agtttttggt
     1861 gcattcaaag ctgttcacca tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca
     1921 cctaatagaa caggtgaaac cagtaaaaca aagcaactag aacatgaaat tgaacacctg
     1981 agacaacttg ttacagctca acagtcacac atagacagcc tgaaacaggc gatgctgctt
     2041 atcgaatcaa agctgccgac aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca
     2101 tggcaattct ggaagaaata gcgctttcag ccggcaaacc ggctgaagcc ggatctgcga
     2161 ttctgataac aaactagcaa caccagaaca gcccgtttgc gggcagcaaa acccgt
//