BBa_K864051 1 pSC101ts pSC101ts temperature sensitive low copy origin of replication 2012-09-25T11:00:00Z 2015-05-08T01:13:37Z pSIM5 pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. false false _1124_ 0 9827 9 Not in stock true - false Erik Lundin annotation2202713 1 Ins(GGC) range2202713 1 2141 2143 annotation2202701 1 Tandem repeat range2202701 1 631 652 annotation2202693 1 Repeat structure range2202693 1 190 204 annotation2202709 1 repA range2202709 1 750 1700 annotation2202688 1 A56V ts mutation range2202688 1 915 917 annotation2202690 1 pSC101ts ori range2202690 1 1 2216 annotation2202708 1 -35 signal range2202708 1 692 697 annotation2202706 1 A>T mutation to remove SpeI range2202706 1 678 678 annotation2202695 1 Repeat structure range2202695 1 232 247 annotation2202703 1 Repeat "4" range2202703 1 671 693 annotation2202692 1 par region range2202692 1 1 368 annotation2202698 1 Repeated sequence range2202698 1 582 589 annotation2202707 1 Hairpin structure range2202707 1 683 761 annotation2202712 1 Hairpin structure range2202712 1 1608 1682 annotation2202710 1 Repeat "5" range2202710 1 702 723 annotation2202700 1 Repeated sequence range2202700 1 603 610 annotation2202705 1 Repeated sequence range2202705 1 676 683 annotation2202711 1 Repeated sequence range2202711 1 712 719 annotation2202697 1 Tandem repeat range2202697 1 578 599 annotation2202699 1 Tandem repeat range2202699 1 601 620 annotation2202702 1 Repeated sequence range2202702 1 635 642 annotation2202696 1 AT rich region range2202696 1 499 581 BBa_K864051_sequence 1 gacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggtctagtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgttcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgacaatgtaacagatgaacagcatgtaacacctaatagaacaggtgaaaccagtaaaacaaagcaactagaacatgaaattgaacacctgagacaacttgttacagctcaacagtcacacatagacagcctgaaacaggcgatgctgcttatcgaatcaaagctgccgacaacacgggagccagtgacgcctcccgtggggaaaaaatcatggcaattctggaagaaatagcgctttcagccggcaaaccggctgaagccggatctgcgattctgataacaaactagcaacaccagaacagcccgtttgcgggcagcaaaacccgt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z