##gff-version 3
##sequence-region BBa_K864120 1 1751
BBa_K864120	.	sequence_feature	1	674	.	+	0	ID=BBa_I50022;Name=BBa_I50022
BBa_K864120	.	sequence_feature	16	630	.	+	0	ID=annotation1959117;Name=rep (pMB1);Parent=BBa_I50022
BBa_K864120	.	sequence_feature	27	615	.	+	0	ID=annotation1959119;Name=origin of replication;Parent=BBa_I50022
BBa_K864120	.	promoter	48	53	.	+	0	ID=annotation1959120;Name=RNAII transcript promoter -10 sequence;Parent=BBa_I50022
BBa_K864120	.	sequence_feature	66	173	.	+	0	ID=annotation1959122;Name=RNAI transcript;Parent=BBa_I50022
BBa_K864120	.	sequence_feature	615	615	.	+	0	ID=annotation1959125;Name=ORI;Parent=BBa_I50022
BBa_K864120	.	sequence_feature	63	615	.	+	0	ID=annotation1959121;Name=RNAII transcript;Parent=BBa_I50022
BBa_K864120	.	promoter	27	32	.	+	0	ID=annotation1959118;Name=RNAII transcript promoter -35 sequence;Parent=BBa_I50022
BBa_K864120	.	promoter	182	187	.	+	0	ID=annotation1959123;Name=RNAI transcript promoter -10;Parent=BBa_I50022
BBa_K864120	.	promoter	204	209	.	+	0	ID=annotation1959124;Name=RNAI transcript promoter -35;Parent=BBa_I50022
BBa_K864120	.	engineered_region	683	1751	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K864120	.	terminator	1623	1702	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K864120	.	engineered_region	1623	1702	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K864120	.	stem_loop	1634	1677	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K864120	.	promoter	683	882	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K864120	.	non_covalent_binding_site	771	808	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K864120	.	promoter	843	848	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K864120	.	engineered_region	683	882	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K864120	.	CDS	683	770	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K864120	.	non_covalent_binding_site	848	882	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K864120	.	promoter	819	824	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K864120	.	start_codon	855	855	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K864120	.	terminator	1711	1751	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K864120	.	engineered_region	1711	1751	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K864120	.	stop_codon	1744	1744	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K864120	.	stem_loop	1718	1737	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K864120	.	polyA_site	1738	1751	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K864120	.	CDS	909	1589	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K864120	.	engineered_tag	1590	1614	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K864120	.	CDS	909	1583	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K864120	.	ribosome_entry_site	891	902	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K864120	.	conserved_region	895	898	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K864120
cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccac
cgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgca
gataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctc
gctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagt
taccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccga
actgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagc
ggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttc
gccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggc
ctttttacggttcctggccttttgctggccttttgctcacattactagagcaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtga
gttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggata
acaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtt
tcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaagg
tacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccag
tacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaat
gggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcat
ctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagct
tccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtc
actacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaa
actggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggt
gcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttata