LOCUS       BBa_K864121             1610 bp    DNA     linear       26-Dec-2024
DEFINITION  pMB1 ori - constitutive RFP
ACCESSION   BBa_K864121
VERSION     BBa_K864121.1  
FEATURES             Location/Qualifiers
     misc_feature    16..630
                     /label=rep (pMB1)
     promoter        27..32
                     /label=RNAII transcript promoter -35 sequence
     misc_feature    27..615
                     /label=origin of replication
     promoter        48..53
                     /label=RNAII transcript promoter -10 sequence
     misc_feature    63..615
                     /label=RNAII transcript
     misc_feature    66..173
                     /label=RNAI transcript
     promoter        182..187
                     /label=RNAI transcript promoter -10
     promoter        204..209
                     /label=RNAI transcript promoter -35
     misc_feature    615..615
                     /label=ORI
     misc_feature    1..674
                     /label=BBa_I50022
     promoter        683..741
                     /label=BBa_K864511
     misc_feature    754..757
                     /label=conserved
     RBS             750..761
                     /label=BBa_B0034
     CDS             768..1442
                     /label=mrfp1
     misc_feature    1449..1473
                     /label=Help:Barcodes
     CDS             768..1473
                     /label=BBa_E1010
     stem_loop       1493..1536
                     /label=stem_loop
     misc_feature    1482..1561
                     /label=BBa_B0010
     terminator      1482..1561
                     /label=BBa_B0010
     stem_loop       1577..1596
                     /label=T7 TE
     misc_feature    1603..1603
                     /label=stop
     polyA_site      1597..1610
                     /label=polya
     misc_feature    1570..1610
                     /label=BBa_B0012
     terminator      1570..1610
                     /label=BBa_B0012
     misc_feature    683..1610
                     /label=BBa_K864502
ORIGIN
        1 cccgtagaaa agatcaaaag atcttcttga gatccttttt ttctgcgcgt aatctgctac
       61 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      121 actctttttc cgaaggtaac tggctttagc agagcgcaga taccaaatac tgtccttcta
      181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      661 ccttttgctc acattactag agcatcgggt agtttattct tgacaattaa gtagagcctg
      721 atataatagt tcagtactgt ttactagaga aagaggagaa atactagatg gcttcctccg
      781 aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc
      841 acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta
      901 aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt
      961 tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac
     1021 tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg
     1081 ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc
     1141 gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag
     1201 cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc
     1261 tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa
     1321 aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca
     1381 acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg
     1441 cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg
     1501 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1561 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//