##gff-version 3
##sequence-region BBa_K864121 1 1610
BBa_K864121	.	sequence_feature	1	674	.	+	0	ID=BBa_I50022;Name=BBa_I50022
BBa_K864121	.	sequence_feature	615	615	.	+	0	ID=annotation1959125;Name=ORI;Parent=BBa_I50022
BBa_K864121	.	sequence_feature	66	173	.	+	0	ID=annotation1959122;Name=RNAI transcript;Parent=BBa_I50022
BBa_K864121	.	promoter	204	209	.	+	0	ID=annotation1959124;Name=RNAI transcript promoter -35;Parent=BBa_I50022
BBa_K864121	.	sequence_feature	16	630	.	+	0	ID=annotation1959117;Name=rep (pMB1);Parent=BBa_I50022
BBa_K864121	.	promoter	27	32	.	+	0	ID=annotation1959118;Name=RNAII transcript promoter -35 sequence;Parent=BBa_I50022
BBa_K864121	.	sequence_feature	27	615	.	+	0	ID=annotation1959119;Name=origin of replication;Parent=BBa_I50022
BBa_K864121	.	promoter	182	187	.	+	0	ID=annotation1959123;Name=RNAI transcript promoter -10;Parent=BBa_I50022
BBa_K864121	.	sequence_feature	63	615	.	+	0	ID=annotation1959121;Name=RNAII transcript;Parent=BBa_I50022
BBa_K864121	.	promoter	48	53	.	+	0	ID=annotation1959120;Name=RNAII transcript promoter -10 sequence;Parent=BBa_I50022
BBa_K864121	.	engineered_region	683	1610	.	+	0	ID=BBa_K864502;Name=BBa_K864502
BBa_K864121	.	promoter	683	741	.	+	0	ID=BBa_K864511;Name=BBa_K864511;Parent=BBa_K864502
BBa_K864121	.	terminator	1570	1610	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K864502
BBa_K864121	.	stem_loop	1577	1596	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K864121	.	engineered_region	1570	1610	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K864121	.	stop_codon	1603	1603	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K864121	.	polyA_site	1597	1610	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K864121	.	terminator	1482	1561	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K864502
BBa_K864121	.	engineered_region	1482	1561	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K864121	.	stem_loop	1493	1536	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K864121	.	CDS	768	1473	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K864502
BBa_K864121	.	CDS	768	1442	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K864121	.	engineered_tag	1449	1473	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K864121	.	ribosome_entry_site	750	761	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K864502
BBa_K864121	.	conserved_region	754	757	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K864121
cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccac
cgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgca
gataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctc
gctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagt
taccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccga
actgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagc
ggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttc
gccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggc
ctttttacggttcctggccttttgctggccttttgctcacattactagagcatcgggtagtttattcttgacaattaag
tagagcctgatataatagttcagtactgtttactagagaaagaggagaaatactagatggcttcctccgaagacgttat
caaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaa
ggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc
tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcctt
cccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctg
caagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaa
ccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaa
actgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgct
tacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaag
gtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata