LOCUS       BBa_K864150             2214 bp    DNA     linear       16-Feb-2025
DEFINITION  Cat-SacB cassette for scarless chromosomal knockout
ACCESSION   BBa_K864150
VERSION     BBa_K864150.1  
FEATURES             Location/Qualifiers
     CDS             107..769
                     /label=cmR
     misc_feature    1..769
                     /label=BBa_P1004
     RBS             778..780
                     /label=RBS
     CDS             790..2211
                     /label=Coding sequence
     misc_feature    988..993
                     /label=Removed EcoRI site
     misc_feature    778..2214
                     /label=BBa_K322921
ORIGIN
        1 gttgatcggg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg
       61 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
      121 cacgggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
      181 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
      241 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
      301 cctgatgaac gctcacccgg agtttcgtat ggccatgaaa gacggtgagc tggtgatctg
      361 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcgtccct
      421 ctggagtgaa taccacgacg atttccggca gtttctccac atatattcgc aagatgtggc
      481 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgttttttgt
      541 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
      601 cttcttcgcc cccgttttca cgatgggcaa atattatacg caaggcgaca aggtgctgat
      661 gccgctggcg atccaggttc atcatgccgt ttgtgatggc ttccatgtcg gccgcatgct
      721 taatgaatta caacagtact gtgatgagtg gcagggcggg gcgtaataat actagaggag
      781 acatgaacga tgaacatcaa aaagtttgca aaacaagcaa cagtattaac ctttactacc
      841 gcactgctgg caggaggcgc aactcaagcg tttgcgaaag aaacgaacca aaagccatat
      901 aaggaaacat acggcatttc ccatattaca cgccatgata tgctgcaaat ccctgaacag
      961 caaaaaaatg aaaaatatca agttcctgag ttcgattcgt ccacaattaa aaatatctct
     1021 tctgcaaaag gcctggacgt ttgggacagc tggccattac aaaacgctga cggcactgtc
     1081 gcaaactatc acggctacca catcgtcttt gcattagccg gagatcctaa aaatgcggat
     1141 gacacatcga tttacatgtt ctatcaaaaa gtcggcgaaa cttctattga cagctggaaa
     1201 aacgctggcc gcgtctttaa agacagcgac aaattcgatg caaatgattc tatcctaaaa
     1261 gaccaaacac aagaatggtc aggttcagcc acatttacat ctgacggaaa aatccgttta
     1321 ttctacactg atttctccgg taaacattac ggcaaacaaa cactgacaac tgcacaagtt
     1381 aacgtatcag catcagacag ctctttgaac atcaacggtg tagaggatta taaatcaatc
     1441 tttgacggtg acggaaaaac gtatcaaaat gtacagcagt tcatcgatga aggcaactac
     1501 agctcaggcg acaaccatac gctgagagat cctcactacg tagaagataa aggccacaaa
     1561 tacttagtat ttgaagcaaa cactggaact gaagatggct accaaggcga agaatcttta
     1621 tttaacaaag catactatgg caaaagcaca tcattcttcc gtcaagaaag tcaaaaactt
     1681 ctgcaaagcg ataaaaaacg cacggctgag ttagcaaacg gcgctctcgg tatgattgag
     1741 ctaaacgatg attacacact gaaaaaagtg atgaaaccgc tgattgcatc taacacagta
     1801 acagatgaaa ttgaacgcgc gaacgtcttt aaaatgaacg gcaaatggta cctgttcact
     1861 gactcccgcg gatcaaaaat gacgattgac ggcattacgt ctaacgatat ttacatgctt
     1921 ggttatgttt ctaattcttt aactggccca tacaagccgc tgaacaaaac tggccttgtg
     1981 ttaaaaatgg atcttgatcc taacgatgta acctttactt actcacactt cgctgtacct
     2041 caagcgaaag gaaacaatgt cgtgattaca agctatatga caaacagagg attctacgca
     2101 gacaaacaat caacgtttgc gccaagcttc ctgctgaaca tcaaaggcaa gaaaacatct
     2161 gttgtcaaag acagcatcct tgaacaagga caattaacag ttaacaaata ataa
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