BBa_K864204 1 BBa_K864204 OmpF 2012-09-24T11:00:00Z 2015-05-08T01:13:37Z Amplified from MG1655 (accession NC_000913) chromosome using primers listed below (annealing sequences are <u>underlined</u>) ompF_BB_F TTCGAATTCGCGGCCGCTTCTAG<u>ATGATGAAGCGCAATATTC</u> ompF_BB_R CGGCCGCTACTAGTATTA<u>TTAGAACTGGTAAACGATACC</u> Released HQ 2013 OmpF false false _1124_ 0 9827 9 In stock false Illegal PstI restriction site removed by silent mutations T>A at position 66 and A>T at position 69 using primers listed below (annealing sequences are <u>underlined</u>) ompF_rsr_F CT<u>GAAATCTATAACAAAGATGGCAA</u> ompF_rsr_R CT<u>GCGTTTGCAGTACCTGCT</u> false Erik Lundin annotation2197199 1 ompF range2197199 1 1 1092 annotation2197200 1 T>A range2197200 1 66 66 annotation2197201 1 A>T range2197201 1 69 69 BBa_K864204_sequence 1 atgatgaagcgcaatattctggcagtgatcgtccctgctctgttagtagcaggtactgcaaacgcagctgaaatctataacaaagatggcaacaaagtagatctgtacggtaaagctgttggtctgcattatttttccaagggtaacggtgaaaacagttacggtggcaatggcgacatgacctatgcccgtcttggttttaaaggggaaactcaaatcaattccgatctgaccggttatggtcagtgggaatataacttccagggtaacaactctgaaggcgctgacgctcaaactggtaacaaaacgcgtctggcattcgcgggtcttaaatacgctgacgttggttctttcgattacggccgtaactacggtgtggtttatgatgcactgggttacaccgatatgctgccagaatttggtggtgatactgcatacagcgatgacttcttcgttggtcgtgttggcggcgttgctacctatcgtaactccaacttctttggtctggttgatggcctgaacttcgctgttcagtacctgggtaaaaacgagcgtgacactgcacgccgttctaacggcgacggtgttggcggttctatcagctacgaatacgaaggctttggtatcgttggtgcttatggtgcagctgaccgtaccaacctgcaagaagctcaacctcttggcaacggtaaaaaagctgaacagtgggctactggtctgaagtacgacgcgaacaacatctacctggcagcgaactacggtgaaacccgtaacgctacgccgatcactaataaatttacaaacaccagcggcttcgccaacaaaacgcaagacgttctgttagttgcgcaataccagttcgatttcggtctgcgtccgtccatcgcttacaccaaatctaaagcgaaagacgtagaaggtatcggtgatgttgatctggtgaactactttgaagtgggcgcaacctactacttcaacaaaaacatgtccacctatgttgactacatcatcaaccagatcgattctgacaacaaactgggcgtaggttcagacgacaccgttgctgtgggtatcgtttaccagttctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z