BBa_K864444 1 BBa_K864444 RFP-Linker-SYFP2 2012-09-24T11:00:00Z 2015-05-08T01:13:37Z Constructed from SYFP2 <partinfo>BBa_K864100</partinfo>, linker <partinfo>BBa_J18922</partinfo> and RFP <partinfo>BBa_J04450</partinfo> Released HQ 2013 Use this part to insert any 5?????TR of your gene of interest to screen for downregulation of your gene with small RNA. PCR amplify your 5???UTR with EcoRI and BamHI and clone into any backbone carrying this part. Digest whis part with EcoRI and BamHI and as RFP is replaced, this part functions as a red/white screening system. This allows you to create a reporter system with a fluorescent marker for screening for functional small RNAs. SYFP2 <partinfo>BBa_K864100</partinfo> is situated downstrean of the RFP <partinfo>BBa_J04450</partinfo> and is connected in frame with a 12 aa glycine-serine repeated linker <partinfo>BBa_J18922</partinfo>, to prevent folding distruptions in the YFP or the 5???UTR. The first 6 nt of the glycine-serine linker is a BamHI restriction site (GGATCC), which translates into glycine-serine in E.Coli. When screening for working small RNAs, it's recommended to include the first 15 codons of the gene of interest. false false _1124_ 0 13998 9 In stock true - false Joel ??s annotation2198889 1 B0012 range2198889 1 1029 1069 annotation2198883 1 RFP J04450 range2198883 1 1 1068 annotation2198886 1 B0034 range2198886 1 209 220 annotation2198885 1 Plac range2198885 1 1 200 annotation2198894 1 BamHI range2198894 1 1070 1075 annotation2198896 1 SYFP2 range2198896 1 1106 1825 annotation2198890 1 B0010 range2198890 1 941 1020 annotation2198887 1 RFP range2198887 1 227 681 annotation2198893 1 Linker range2198893 1 1076 1105 BBa_K864444_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggatccggtagcggcagcggtagcggtagcggcagcatggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z