BBa_K864446 1 BBa_K864446 Spot42 randomized reverse primer 2012-09-28T11:00:00Z 2015-05-08T01:13:37Z Own design. This part is a component of the Uppsala University iGEM 2012 modular small RNA screening system. This primer is used to create a random sRNA library, together with <partinfo>K864445</partinfo>, by randomizing the mRNA-binding region of the spot42 small RNA (<partinfo>K864444</partinfo>). This library can be used to screen for synthetic silencing sRNA:s. '''Note''' Due to technical limitations, the correct sequence can not be entered into the registry. The entered sequence should be preceded by 20 N:s, to denote the randomized region, and thus read: NNNNNNNNNNNNNNNNNNNNGCTAGCATAATACCTAGGAC false false _1124_ 0 13993 9 Not in stock false The melting temperature has been designed to match <partinfo>K864445</partinfo>. false Arvid Hed??n Gynn??, Sabri Jamal, Lisa Branzell, Hampus Elofsson BBa_K864446_sequence 1 gctagcataatacctaggac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z