LOCUS       BBa_K872001             1982 bp    DNA     linear       01-Jun-2024
DEFINITION  CO2 biosensor
ACCESSION   BBa_K872001
VERSION     BBa_K872001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             50..53
                     /label=RBS
     misc_feature    65..99
                     /label=BBa_J23101
     RBS             108..120
                     /label=RBS-3\Weak
     misc_feature    108..120
                     /label=BBa_B0032
     RBS             114..117
                     /label=RBS
     misc_feature    129..163
                     /label=BBa_J23101
     misc_feature    172..184
                     /label=BBa_B0032
     RBS             172..184
                     /label=RBS-3\Weak
     RBS             178..181
                     /label=RBS
     misc_feature    191..1047
                     /label=BBa_K872000
     misc_feature    1056..1184
                     /label=BBa_B0015
     misc_feature    1056..1135
                     /label=BBa_B0010
     stem_loop       1067..1110
                     /label=stem_loop
     misc_feature    1144..1184
                     /label=BBa_B0012
     stem_loop       1151..1170
                     /label=T7 TE
     polyA_site      1171..1184
                     /label=polya
     misc_feature    1177..1177
                     /label=stop
     misc_feature    1193..1321
                     /label=BBa_B0015
     misc_feature    1193..1272
                     /label=BBa_B0010
     stem_loop       1204..1247
                     /label=stem_loop
     misc_feature    1281..1321
                     /label=BBa_B0012
     stem_loop       1288..1307
                     /label=T7 TE
     polyA_site      1308..1321
                     /label=polya
     misc_feature    1314..1314
                     /label=stop
     misc_feature    1330..1409
                     /label=BBa_B0010
     misc_feature    1330..1458
                     /label=BBa_B0015
     stem_loop       1341..1384
                     /label=stem_loop
     misc_feature    1418..1458
                     /label=BBa_B0012
     stem_loop       1425..1444
                     /label=T7 TE
     polyA_site      1445..1458
                     /label=polya
     misc_feature    1451..1451
                     /label=stop
     misc_feature    1467..1487
                     /label=Crp-cAMP
     misc_feature    1467..1517
                     /label=BBa_K398326
     misc_feature    1526..1538
                     /label=BBa_B0032
     RBS             1526..1538
                     /label=RBS-3\Weak
     RBS             1532..1535
                     /label=RBS
     misc_feature    1545..2264
                     /label=BBa_E0040
     CDS             1545..2264
                     /label=GFP protein
     misc_feature    2273..2352
                     /label=BBa_B0010
     stem_loop       2284..2327
                     /label=stem_loop
     misc_feature    2361..2401
                     /label=BBa_B0012
     stem_loop       2368..2387
                     /label=T7 TE
     polyA_site      2388..2401
                     /label=polya
     misc_feature    2394..2394
                     /label=stop
     misc_feature    1467..2401
                     /label=BBa_K398331
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagcaaaga ggagaaaatg agcctatgga
       61 atgatttgcg attatttttc actcttgtca ttatactcgt attcatttta attacaattc
      121 aaagccgtcg atcagaactg attgctcggt ttgactttat ttggaagctt caggcattag
      181 acgaaggaaa agaaatggaa aaaagacatg cgcaaaatcg agctgtttta gaaaatattt
      241 tgccagcgca tgttgccgaa tatttcctta aagaaactga aagggctgaa ttgtactcag
      301 aagctcgaga taatgctgca atagtattta ttacaattac tgaatttgat aaattttata
      361 tggaattgga tgctaacaat gagggtgttg aatgtttacg acttcttaat gagattattg
      421 ccgactttga tatgcaactc agtcgtgatg agtttaaatg cattgaaaag atcaaaacaa
      481 tctcgactac atatatggca gcatcaggct tatttggtaa agtaaccggt tattcacatg
      541 ttgtcgccgt tgtattattt gctattcgcc ttttagccct tatacaatat atcaatgaac
      601 attcgtttaa taattttaat cttaggatag gcattaatgt tggtccggtg gtagcaggtg
      661 ttattggtgt caagaagcca cattatgata tttggggtaa ctcggtcaac gttgctagca
      721 gaatggatag ttctggtgta gcgggcaaaa tacaaataac ggaagaaacg aaaaatatct
      781 tagaaaaaga aggcttcgag tttgaatgcc gtggaataat taatgtaaaa ggtaaaggtg
      841 atatgaagac atacttcatt aaagtatcag aagaagactt gaattttgat tttaataaga
      901 tctgaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      961 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1021 tttctgcgtt tataggatcc tctagtaagc aggatttagc tcacacttat cgacggtgaa
     1081 gttgcatact atcgatatac tagagtcaca caggaaagta ctagatgcgt aaaggagaag
     1141 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
     1201 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
     1261 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
     1321 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
     1381 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
     1441 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
     1501 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
     1561 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
     1621 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
     1681 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     1741 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
     1801 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agaccaggca
     1861 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1921 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1981 ta
//