LOCUS       BBa_K873003             1302 bp    DNA     linear       06-May-2024
DEFINITION  conjugative transfer of RFG
ACCESSION   BBa_K873003
VERSION     BBa_K873003.1  
FEATURES             Location/Qualifiers
     oriT            1..371
                     /label=BBa_J01003
     misc_feature    380..398
                     /label=TetR 1
     misc_feature    380..433
                     /label=BBa_R0040
     promoter        399..404
                     /label=-35
     misc_feature    405..423
                     /label=TetR 2
     promoter        422..427
                     /label=-10
     promoter        380..433
                     /label=BBa_R0040
     misc_feature    446..449
                     /label=conserved
     RBS             442..453
                     /label=BBa_B0034
     CDS             460..1134
                     /label=mrfp1
     misc_feature    1141..1165
                     /label=Help:Barcodes
     CDS             460..1140
                     /label=BBa_E1010
     stem_loop       1185..1228
                     /label=stem_loop
     misc_feature    1174..1253
                     /label=BBa_B0010
     terminator      1174..1253
                     /label=BBa_B0010
     stem_loop       1269..1288
                     /label=T7 TE
     misc_feature    1295..1295
                     /label=stop
     polyA_site      1289..1302
                     /label=polya
     misc_feature    1262..1302
                     /label=BBa_B0012
     terminator      1262..1302
                     /label=BBa_B0012
     misc_feature    380..1302
                     /label=BBa_I13521
ORIGIN
        1 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg
       61 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
      121 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
      181 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
      241 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
      301 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
      361 gcagcggaaa atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag
      421 agatactgag cactactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt
      481 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
      541 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
      601 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
      661 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
      721 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
      781 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
      841 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
      901 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
      961 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
     1021 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
     1081 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
     1141 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc
     1201 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1261 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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