##gff-version 3
##sequence-region BBa_K873003 1 1302
BBa_K873003	.	oriT	1	371	.	+	0	ID=BBa_J01003;Name=BBa_J01003
BBa_K873003	.	engineered_region	380	1302	.	+	0	ID=BBa_I13521;Name=BBa_I13521
BBa_K873003	.	terminator	1174	1253	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_K873003	.	stem_loop	1185	1228	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K873003	.	engineered_region	1174	1253	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K873003	.	ribosome_entry_site	442	453	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_K873003	.	conserved_region	446	449	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K873003	.	terminator	1262	1302	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_K873003	.	polyA_site	1289	1302	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K873003	.	stem_loop	1269	1288	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K873003	.	stop_codon	1295	1295	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K873003	.	engineered_region	1262	1302	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K873003	.	promoter	380	433	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_K873003	.	non_covalent_binding_site	380	398	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K873003	.	non_covalent_binding_site	405	423	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K873003	.	promoter	399	404	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K873003	.	engineered_region	380	433	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K873003	.	promoter	422	427	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K873003	.	CDS	460	1140	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_K873003	.	engineered_tag	1141	1165	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K873003	.	CDS	460	1134	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
>BBa_K873003
gccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggct
tggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgtt
gagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgccc
ggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatg
gatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaatactagagtccctatcagtgatag
agattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaa
gacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtg
aaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttg
ggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaa
ctgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggact
cctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgca
gaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatg
cgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgc
cgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacg
tgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata