LOCUS       BBa_K874100             1154 bp    DNA     linear       17-May-2024
DEFINITION  IPTG inducible expression of M.ScaI methyltransferase (IPTG -> 
            M.ScaI)
ACCESSION   BBa_K874100
VERSION     BBa_K874100.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     misc_feature    137..139
                     /label=start
     CDS             137..1048
                     /label=M.ScaI
     misc_feature    1049..1051
                     /label=stop
     CDS             137..1051
                     /label=BBa_K874000
     misc_feature    64..1051
                     /label=BBa_S05066
     stem_loop       1067..1086
                     /label=T7 TE
     misc_feature    1093..1093
                     /label=stop
     polyA_site      1087..1100
                     /label=polya
     misc_feature    1060..1100
                     /label=BBa_B0012
     terminator      1060..1100
                     /label=BBa_B0012
     stem_loop       1121..1143
                     /label=stem_loop
     misc_feature    1109..1154
                     /label=BBa_B0011
     terminator      1109..1154
                     /label=BBa_B0011
     terminator      1060..1154
                     /label=BBa_B0014
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagcgca tggccgccag ctttcaagaa cagaaactca tctctgaaga
      121 ggatctggca cccggcatgt ccgggcggga ctttggatat gtgatacagt cgtccgctgc
      181 actatggaat cgactctcta cattctcaca gagaggaaaa gccttggaca ccaggcttgc
      241 agacatcaag aaggccctgg ggaagccgta ctacgaaacc tcggatgtcc ttctttacca
      301 cggcgacagt cttgagctgc tcaagtcaat gcctcagcag attttcgacc ttaccgtaac
      361 tagcccacct tacaatattg gcaaagagta cgagggtgta ctgtcgatcg aggaatacat
      421 ttcctggtgc gagacatgga tgtcgcgcgt tcatagggcg accagcgcag gcggcgcatt
      481 ttggctcaat gttgggtacg tccctgtccc gaaccaagga aaagcagtcc cgattcctta
      541 cctcttgtgg gacaagagtc cgttctacat gatccaggaa gttgtctgga attacggggc
      601 gggagtggcg tctcgaaaat cgttttcccc gcgcaatgaa aagtttctct ggtatgtgcg
      661 cgacccgctg aattattact tcgacctcga ttcggtgcgc gacccaaatg tgaaataccc
      721 caaccagaaa aagaatggga agctcaaatg caacccgttg gggaaaaatc ccactgacgt
      781 ttggcagttc cccaaggtta cgtcgggcgc gaagagatca agcgtggagc gcaccgccca
      841 tccggcacaa ttcccgtctg ctgtcattga acgggtcatc aaggcgtgca gcccttccga
      901 cggcgtcatc ctggacccat tcctcggttc cggaacgacc tcgctgaccg ccagaaagca
      961 aggccggtgc agcgtcggta tcgaaatccg cgaagactac ctcgacatcg cggtgggacg
     1021 cctggaggcg gaggcgcaat ccctcttcta gtactagagt cacactggct caccttcggg
     1081 tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg
     1141 cttttttatt attt
//