LOCUS       BBa_K874101             1229 bp    DNA     linear       15-May-2024
DEFINITION  Arabinose inducible expression of M.ScaI methyltransferase (ARA -> 
            M.ScaI)
ACCESSION   BBa_K874101
VERSION     BBa_K874101.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206001
     RBS             139..151
                     /label=RBS-3\Weak
     RBS             145..148
                     /label=RBS
     misc_feature    139..151
                     /label=BBa_B0032
     RBS             139..151
                     /label=BBa_B0032
     misc_feature    212..214
                     /label=start
     CDS             212..1123
                     /label=M.ScaI
     misc_feature    1124..1126
                     /label=stop
     CDS             212..1126
                     /label=BBa_K874000
     misc_feature    139..1126
                     /label=BBa_S05066
     stem_loop       1142..1161
                     /label=T7 TE
     misc_feature    1168..1168
                     /label=stop
     polyA_site      1162..1175
                     /label=polya
     misc_feature    1135..1175
                     /label=BBa_B0012
     terminator      1135..1175
                     /label=BBa_B0012
     stem_loop       1196..1218
                     /label=stem_loop
     misc_feature    1184..1229
                     /label=BBa_B0011
     terminator      1184..1229
                     /label=BBa_B0011
     terminator      1135..1229
                     /label=BBa_B0014
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agctttttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagtc acacaggaaa gcgcatggcc gccagctttc aagaacagaa
      181 actcatctct gaagaggatc tggcacccgg catgtccggg cgggactttg gatatgtgat
      241 acagtcgtcc gctgcactat ggaatcgact ctctacattc tcacagagag gaaaagcctt
      301 ggacaccagg cttgcagaca tcaagaaggc cctggggaag ccgtactacg aaacctcgga
      361 tgtccttctt taccacggcg acagtcttga gctgctcaag tcaatgcctc agcagatttt
      421 cgaccttacc gtaactagcc caccttacaa tattggcaaa gagtacgagg gtgtactgtc
      481 gatcgaggaa tacatttcct ggtgcgagac atggatgtcg cgcgttcata gggcgaccag
      541 cgcaggcggc gcattttggc tcaatgttgg gtacgtccct gtcccgaacc aaggaaaagc
      601 agtcccgatt ccttacctct tgtgggacaa gagtccgttc tacatgatcc aggaagttgt
      661 ctggaattac ggggcgggag tggcgtctcg aaaatcgttt tccccgcgca atgaaaagtt
      721 tctctggtat gtgcgcgacc cgctgaatta ttacttcgac ctcgattcgg tgcgcgaccc
      781 aaatgtgaaa taccccaacc agaaaaagaa tgggaagctc aaatgcaacc cgttggggaa
      841 aaatcccact gacgtttggc agttccccaa ggttacgtcg ggcgcgaaga gatcaagcgt
      901 ggagcgcacc gcccatccgg cacaattccc gtctgctgtc attgaacggg tcatcaaggc
      961 gtgcagccct tccgacggcg tcatcctgga cccattcctc ggttccggaa cgacctcgct
     1021 gaccgccaga aagcaaggcc ggtgcagcgt cggtatcgaa atccgcgaag actacctcga
     1081 catcgcggtg ggacgcctgg aggcggaggc gcaatccctc ttctagtact agagtcacac
     1141 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc
     1201 agattattaa tccggctttt ttattattt
//