BBa_K874000 1 BBa_K874000 M.ScaI Methyltransferase 2012-09-16T11:00:00Z 2015-05-08T01:13:38Z Natively found in Streptomyces caespitosus. Sequence obtained from REBASE. Synthesized by IDT. The M.ScaI protein is a type II methyltransferase (subtype beta) that recognises site on the DNA of the following sequence 5..AGTACT..3. It methylates this site at the 5th (Cytosine) nucleotide leaving ao an N4-methylcytosine (m4). This methylation type (m4) is not found in native E. coli nor is the recognition site methylated by any of E. coli's native methylation systems (Dam, Dcm). This part also includes a flexible linker with incorporated myc-tag at the N terminus of the protein. This will allow for easier creation of fusion proteins and expression verification using westerblot (using the myc antibodies). It can be assumed that M.ScaI (with linker) is expressed and folds properly in E. coli because its function has been verified by the iGEM Amsterdam 2012 team. false false _1135_ 0 12435 9 Not in stock true Contains no forbidden sites (according to the RFC-10 protocol). Contains no sites vulnerable to E. coli's native restriction systems. false Ernst Bank annotation2187003 1 start range2187003 1 1 3 annotation2187004 1 M.ScaI range2187004 1 1 912 annotation2187005 1 stop range2187005 1 913 915 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K874001 1 BBa_K874001 Polydactyl Zinc Finger (PZF3838) 2012-09-18T11:00:00Z 2015-05-08T01:13:38Z Synthesized by IDT. For more information see individual components descriptions. Intermediate construct used in the iGEM Amsterdam 2012 project. false true _1135_ 0 12435 9 Not in stock false See Individual components false Ernst Bank annotation2200705 1 Zinc Finger 5 range2200705 1 343 426 annotation2200707 1 Zinc Finger 6 range2200707 1 427 510 annotation2192156 1 start range2192156 1 4 6 annotation2200695 1 Zinc Finger 1 range2200695 1 7 90 annotation2200703 1 Zinc Finger 4 range2200703 1 259 342 annotation2200700 1 Zinc Finger 3 range2200700 1 175 258 annotation2192157 1 Zinc Finger Array range2192157 1 7 510 annotation2200698 1 Zinc Finger 2 range2200698 1 91 174 BBa_K874102 1 BBa_K874102 IPTG inducible expression of PZF3838, M.ScaI Fusion protein (IPTG -> PZF3838, M.ScaI FP) 2012-09-21T11:00:00Z 2015-05-08T01:13:38Z None yet None yet false false _1135_ 0 12435 9 Not in stock false None yet false Ernst Bank component2192177 1 BBa_R0011 component2192202 1 BBa_B0014 component2192195 1 BBa_S05074 annotation2192177 1 BBa_R0011 range2192177 1 1 54 annotation2192195 1 BBa_S05074 range2192195 1 64 1555 annotation2192202 1 BBa_B0014 range2192202 1 1564 1658 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation1683 1 stem_loop range1683 1 13 35 annotation7019 1 BBa_B0011 range7019 1 1 46 BBa_S05074 1 BBa_S05074 B0032:K874021 2012-09-21T11:00:00Z 2015-05-08T01:14:49Z false false _9_ 0 12435 9 Not in stock false false Ernst Bank component2192176 1 BBa_K874000 component2192172 1 BBa_K874021 component2192169 1 BBa_K874001 component2192165 1 BBa_B0032 annotation2192169 1 BBa_K874001 range2192169 1 14 523 annotation2192165 1 BBa_B0032 range2192165 1 1 13 annotation2192172 1 BBa_K874021 range2192172 1 524 577 annotation2192176 1 BBa_K874000 range2192176 1 578 1492 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2000 1 -35 range2000 1 20 25 annotation2001 1 lac O1 range2001 1 26 42 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2002 1 -10 range2002 1 43 48 annotation1999 1 lac O1 range1999 1 3 19 BBa_K874021 1 BBa_K874021 Myc-Linker 2012-09-21T11:00:00Z 2015-05-08T01:13:38Z None yet None yet false false _1135_ 0 12435 9 Not in stock false None yet false Ernst Bank annotation2191557 1 Linker range2191557 1 1 54 annotation2191558 1 Myc range2191558 1 16 45 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939311 1 BBa_B0011 component939303 1 BBa_B0012 annotation939311 1 BBa_B0011 range939311 1 50 95 annotation939303 1 BBa_B0012 range939303 1 1 41 BBa_K874021_sequence 1 gccgccagctttcaagaacagaaactcatctctgaagaggatctggcacccggc BBa_K874001_sequence 1 cgcatgggcgaaaagccgtatgcgtgccccgaatgtgggaagtccttttctcgtagcgacgagcttgttcgccaccaaaggacccataccggcgagaagccctacgcctgcccggagtgtggaaagtcgttctcccagagcggcgatttgcgtcggcatcaacggacgcataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgagaaaccatacgcctgtcccgagtgcggtaaaagctttagtcagcgtgcccatcttgaacgccaccagaggacccataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgaaaaaccgtatgcttgtccagaatgtggtaagtccttttctcgtagcgataagctcgttcgccaccaaaggactcatacc BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K874000_sequence 1 atgtccgggcgggactttggatatgtgatacagtcgtccgctgcactatggaatcgactctctacattctcacagagaggaaaagccttggacaccaggcttgcagacatcaagaaggccctggggaagccgtactacgaaacctcggatgtccttctttaccacggcgacagtcttgagctgctcaagtcaatgcctcagcagattttcgaccttaccgtaactagcccaccttacaatattggcaaagagtacgagggtgtactgtcgatcgaggaatacatttcctggtgcgagacatggatgtcgcgcgttcatagggcgaccagcgcaggcggcgcattttggctcaatgttgggtacgtccctgtcccgaaccaaggaaaagcagtcccgattccttacctcttgtgggacaagagtccgttctacatgatccaggaagttgtctggaattacggggcgggagtggcgtctcgaaaatcgttttccccgcgcaatgaaaagtttctctggtatgtgcgcgacccgctgaattattacttcgacctcgattcggtgcgcgacccaaatgtgaaataccccaaccagaaaaagaatgggaagctcaaatgcaacccgttggggaaaaatcccactgacgtttggcagttccccaaggttacgtcgggcgcgaagagatcaagcgtggagcgcaccgcccatccggcacaattcccgtctgctgtcattgaacgggtcatcaaggcgtgcagcccttccgacggcgtcatcctggacccattcctcggttccggaacgacctcgctgaccgccagaaagcaaggccggtgcagcgtcggtatcgaaatccgcgaagactacctcgacatcgcggtgggacgcctggaggcggaggcgcaatccctcttctag BBa_S05074_sequence 1 tcacacaggaaagcgcatgggcgaaaagccgtatgcgtgccccgaatgtgggaagtccttttctcgtagcgacgagcttgttcgccaccaaaggacccataccggcgagaagccctacgcctgcccggagtgtggaaagtcgttctcccagagcggcgatttgcgtcggcatcaacggacgcataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgagaaaccatacgcctgtcccgagtgcggtaaaagctttagtcagcgtgcccatcttgaacgccaccagaggacccataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgaaaaaccgtatgcttgtccagaatgtggtaagtccttttctcgtagcgataagctcgttcgccaccaaaggactcataccgccgccagctttcaagaacagaaactcatctctgaagaggatctggcacccggcatgtccgggcgggactttggatatgtgatacagtcgtccgctgcactatggaatcgactctctacattctcacagagaggaaaagccttggacaccaggcttgcagacatcaagaaggccctggggaagccgtactacgaaacctcggatgtccttctttaccacggcgacagtcttgagctgctcaagtcaatgcctcagcagattttcgaccttaccgtaactagcccaccttacaatattggcaaagagtacgagggtgtactgtcgatcgaggaatacatttcctggtgcgagacatggatgtcgcgcgttcatagggcgaccagcgcaggcggcgcattttggctcaatgttgggtacgtccctgtcccgaaccaaggaaaagcagtcccgattccttacctcttgtgggacaagagtccgttctacatgatccaggaagttgtctggaattacggggcgggagtggcgtctcgaaaatcgttttccccgcgcaatgaaaagtttctctggtatgtgcgcgacccgctgaattattacttcgacctcgattcggtgcgcgacccaaatgtgaaataccccaaccagaaaaagaatgggaagctcaaatgcaacccgttggggaaaaatcccactgacgtttggcagttccccaaggttacgtcgggcgcgaagagatcaagcgtggagcgcaccgcccatccggcacaattcccgtctgctgtcattgaacgggtcatcaaggcgtgcagcccttccgacggcgtcatcctggacccattcctcggttccggaacgacctcgctgaccgccagaaagcaaggccggtgcagcgtcggtatcgaaatccgcgaagactacctcgacatcgcggtgggacgcctggaggcggaggcgcaatccctcttctag BBa_B0032_sequence 1 tcacacaggaaag BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_K874102_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagcgcatgggcgaaaagccgtatgcgtgccccgaatgtgggaagtccttttctcgtagcgacgagcttgttcgccaccaaaggacccataccggcgagaagccctacgcctgcccggagtgtggaaagtcgttctcccagagcggcgatttgcgtcggcatcaacggacgcataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgagaaaccatacgcctgtcccgagtgcggtaaaagctttagtcagcgtgcccatcttgaacgccaccagaggacccataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgaaaaaccgtatgcttgtccagaatgtggtaagtccttttctcgtagcgataagctcgttcgccaccaaaggactcataccgccgccagctttcaagaacagaaactcatctctgaagaggatctggcacccggcatgtccgggcgggactttggatatgtgatacagtcgtccgctgcactatggaatcgactctctacattctcacagagaggaaaagccttggacaccaggcttgcagacatcaagaaggccctggggaagccgtactacgaaacctcggatgtccttctttaccacggcgacagtcttgagctgctcaagtcaatgcctcagcagattttcgaccttaccgtaactagcccaccttacaatattggcaaagagtacgagggtgtactgtcgatcgaggaatacatttcctggtgcgagacatggatgtcgcgcgttcatagggcgaccagcgcaggcggcgcattttggctcaatgttgggtacgtccctgtcccgaaccaaggaaaagcagtcccgattccttacctcttgtgggacaagagtccgttctacatgatccaggaagttgtctggaattacggggcgggagtggcgtctcgaaaatcgttttccccgcgcaatgaaaagtttctctggtatgtgcgcgacccgctgaattattacttcgacctcgattcggtgcgcgacccaaatgtgaaataccccaaccagaaaaagaatgggaagctcaaatgcaacccgttggggaaaaatcccactgacgtttggcagttccccaaggttacgtcgggcgcgaagagatcaagcgtggagcgcaccgcccatccggcacaattcccgtctgctgtcattgaacgggtcatcaaggcgtgcagcccttccgacggcgtcatcctggacccattcctcggttccggaacgacctcgctgaccgccagaaagcaaggccggtgcagcgtcggtatcgaaatccgcgaagactacctcgacatcgcggtgggacgcctggaggcggaggcgcaatccctcttctagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z