LOCUS       BBa_K874103             1733 bp    DNA     linear       06-Dec-2025
DEFINITION  Arabinose inducible expression of PZF3838, M.ScaI Fusion protein 
            (ARA -> PZF3838, M.ScaI FP)
ACCESSION   BBa_K874103
VERSION     BBa_K874103.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206001
     RBS             139..151
                     /label=RBS-3\Weak
     RBS             145..148
                     /label=RBS
     misc_feature    139..151
                     /label=BBa_B0032
     RBS             139..151
                     /label=BBa_B0032
     misc_feature    155..157
                     /label=start
     CDS             158..661
                     /label=Zinc Finger Array
     CDS             158..241
                     /label=Zinc Finger 1
     CDS             242..325
                     /label=Zinc Finger 2
     CDS             326..409
                     /label=Zinc Finger 3
     CDS             410..493
                     /label=Zinc Finger 4
     CDS             494..577
                     /label=Zinc Finger 5
     CDS             578..661
                     /label=Zinc Finger 6
     CDS             152..661
                     /label=BBa_K874001
     CDS             662..715
                     /label=Linker
     misc_feature    677..706
                     /label=Myc
     misc_feature    662..715
                     /label=BBa_K874021
     misc_feature    716..718
                     /label=start
     CDS             716..1627
                     /label=M.ScaI
     misc_feature    1628..1630
                     /label=stop
     CDS             716..1630
                     /label=BBa_K874000
     misc_feature    139..1630
                     /label=BBa_S05074
     stem_loop       1646..1665
                     /label=T7 TE
     misc_feature    1672..1672
                     /label=stop
     polyA_site      1666..1679
                     /label=polya
     misc_feature    1639..1679
                     /label=BBa_B0012
     terminator      1639..1679
                     /label=BBa_B0012
     stem_loop       1700..1722
                     /label=stem_loop
     misc_feature    1688..1733
                     /label=BBa_B0011
     terminator      1688..1733
                     /label=BBa_B0011
     terminator      1639..1733
                     /label=BBa_B0014
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agctttttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagtc acacaggaaa gcgcatgggc gaaaagccgt atgcgtgccc
      181 cgaatgtggg aagtcctttt ctcgtagcga cgagcttgtt cgccaccaaa ggacccatac
      241 cggcgagaag ccctacgcct gcccggagtg tggaaagtcg ttctcccaga gcggcgattt
      301 gcgtcggcat caacggacgc ataccggcga gaagccttac gcttgcccgg agtgtggcaa
      361 gtcattcagc gactgccgtg atctcgcaag gcatcaacgc acccataccg gcgagaaacc
      421 atacgcctgt cccgagtgcg gtaaaagctt tagtcagcgt gcccatcttg aacgccacca
      481 gaggacccat accggcgaga agccttacgc ttgcccggag tgtggcaagt cattcagcga
      541 ctgccgtgat ctcgcaaggc atcaacgcac ccataccggc gaaaaaccgt atgcttgtcc
      601 agaatgtggt aagtcctttt ctcgtagcga taagctcgtt cgccaccaaa ggactcatac
      661 cgccgccagc tttcaagaac agaaactcat ctctgaagag gatctggcac ccggcatgtc
      721 cgggcgggac tttggatatg tgatacagtc gtccgctgca ctatggaatc gactctctac
      781 attctcacag agaggaaaag ccttggacac caggcttgca gacatcaaga aggccctggg
      841 gaagccgtac tacgaaacct cggatgtcct tctttaccac ggcgacagtc ttgagctgct
      901 caagtcaatg cctcagcaga ttttcgacct taccgtaact agcccacctt acaatattgg
      961 caaagagtac gagggtgtac tgtcgatcga ggaatacatt tcctggtgcg agacatggat
     1021 gtcgcgcgtt catagggcga ccagcgcagg cggcgcattt tggctcaatg ttgggtacgt
     1081 ccctgtcccg aaccaaggaa aagcagtccc gattccttac ctcttgtggg acaagagtcc
     1141 gttctacatg atccaggaag ttgtctggaa ttacggggcg ggagtggcgt ctcgaaaatc
     1201 gttttccccg cgcaatgaaa agtttctctg gtatgtgcgc gacccgctga attattactt
     1261 cgacctcgat tcggtgcgcg acccaaatgt gaaatacccc aaccagaaaa agaatgggaa
     1321 gctcaaatgc aacccgttgg ggaaaaatcc cactgacgtt tggcagttcc ccaaggttac
     1381 gtcgggcgcg aagagatcaa gcgtggagcg caccgcccat ccggcacaat tcccgtctgc
     1441 tgtcattgaa cgggtcatca aggcgtgcag cccttccgac ggcgtcatcc tggacccatt
     1501 cctcggttcc ggaacgacct cgctgaccgc cagaaagcaa ggccggtgca gcgtcggtat
     1561 cgaaatccgc gaagactacc tcgacatcgc ggtgggacgc ctggaggcgg aggcgcaatc
     1621 cctcttctag tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
     1681 actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta ttt
//