BBa_K874103
type: ComponentDefinition
persistent identity: BBa K874103
display id: BBa_K874103
version: 1
was generated by: iGEM to SBOL conversion
description: Arabinose inducible expression of PZF3838, M.ScaI Fusion protein (ARA -> PZF3838, M.ScaI FP)
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:38Z
mutable provenance: None yet
owned by: james
owned by: myers
mutable description: None yet
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1135_
m user id: 0
owner id: 12435
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None yet
star: false
creator: Ernst Bank
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0014
component: BBa_S05074
component: BBa_K206001
sequence annotation: BBa_K206001
sequence annotation: BBa_S05074
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K874103_sequence/1

BBa_B0014
type: Component
persistent identity: component2192237
display id: component2192237
version: 1
top level: BBa_K874103
definition: BBa_B0014
access: public

BBa_S05074
type: Component
persistent identity: component2192230
display id: component2192230
version: 1
top level: BBa_K874103
definition: BBa_S05074
access: public

BBa_K206001
type: Component
persistent identity: component2192216
display id: component2192216
version: 1
top level: BBa_K874103
definition: BBa_K206001
access: public

BBa_K206001
type: SequenceAnnotation
persistent identity: annotation2192216
display id: annotation2192216
version: 1
top level: BBa_K874103
location: https://synbiohub.org/public/igem/BBa_K874103/annotation2192216/range2192216/1
component: BBa_K206001

https://synbiohub.org/public/igem/BBa_K874103/annotation2192216/range2192216/1
type: Range
persistent identity: range2192216
display id: range2192216
version: 1
top level: BBa_K874103
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_S05074
type: SequenceAnnotation
persistent identity: annotation2192230
display id: annotation2192230
version: 1
top level: BBa_K874103
location: https://synbiohub.org/public/igem/BBa_K874103/annotation2192230/range2192230/1
component: BBa_S05074

https://synbiohub.org/public/igem/BBa_K874103/annotation2192230/range2192230/1
type: Range
persistent identity: range2192230
display id: range2192230
version: 1
top level: BBa_K874103
direction: direction/reverse
start: 139
end: 1630
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2192237
display id: annotation2192237
version: 1
top level: BBa_K874103
location: https://synbiohub.org/public/igem/BBa_K874103/annotation2192237/range2192237/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K874103/annotation2192237/range2192237/1
type: Range
persistent identity: range2192237
display id: range2192237
version: 1
top level: BBa_K874103
direction: direction/reverse
start: 1639
end: 1733
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_S05074
type: ComponentDefinition
persistent identity: BBa S05074
display id: BBa_S05074
version: 1
was generated by: iGEM to SBOL conversion
description: B0032:K874021
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:14:49Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 12435
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Ernst Bank
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K874021
component: BBa_K874001
component: BBa_B0032
component: BBa_K874000
sequence annotation: BBa_B0032
sequence annotation: BBa_K874001
sequence annotation: BBa_K874021
sequence annotation: BBa_K874000
sequence: https://synbiohub.org/public/igem/BBa_S05074_sequence/1

BBa_K874021
type: Component
persistent identity: component2192172
display id: component2192172
version: 1
top level: BBa_S05074
definition: BBa_K874021
access: public

BBa_K874001
type: Component
persistent identity: component2192169
display id: component2192169
version: 1
top level: BBa_S05074
definition: BBa_K874001
access: public

BBa_B0032
type: Component
persistent identity: component2192165
display id: component2192165
version: 1
top level: BBa_S05074
definition: BBa_B0032
access: public

BBa_K874000
type: Component
persistent identity: component2192176
display id: component2192176
version: 1
top level: BBa_S05074
definition: BBa_K874000
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2192165
display id: annotation2192165
version: 1
top level: BBa_S05074
location: https://synbiohub.org/public/igem/BBa_S05074/annotation2192165/range2192165/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_S05074/annotation2192165/range2192165/1
type: Range
persistent identity: range2192165
display id: range2192165
version: 1
top level: BBa_S05074
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K874001
type: SequenceAnnotation
persistent identity: annotation2192169
display id: annotation2192169
version: 1
top level: BBa_S05074
location: https://synbiohub.org/public/igem/BBa_S05074/annotation2192169/range2192169/1
component: BBa_K874001

https://synbiohub.org/public/igem/BBa_S05074/annotation2192169/range2192169/1
type: Range
persistent identity: range2192169
display id: range2192169
version: 1
top level: BBa_S05074
direction: direction/reverse
start: 14
end: 523
orientation: inline

BBa_K874021
type: SequenceAnnotation
persistent identity: annotation2192172
display id: annotation2192172
version: 1
top level: BBa_S05074
location: https://synbiohub.org/public/igem/BBa_S05074/annotation2192172/range2192172/1
component: BBa_K874021

https://synbiohub.org/public/igem/BBa_S05074/annotation2192172/range2192172/1
type: Range
persistent identity: range2192172
display id: range2192172
version: 1
top level: BBa_S05074
direction: direction/reverse
start: 524
end: 577
orientation: inline

BBa_K874000
type: SequenceAnnotation
persistent identity: annotation2192176
display id: annotation2192176
version: 1
top level: BBa_S05074
location: https://synbiohub.org/public/igem/BBa_S05074/annotation2192176/range2192176/1
component: BBa_K874000

https://synbiohub.org/public/igem/BBa_S05074/annotation2192176/range2192176/1
type: Range
persistent identity: range2192176
display id: range2192176
version: 1
top level: BBa_S05074
direction: direction/reverse
start: 578
end: 1492
orientation: inline

BBa_K206001
type: ComponentDefinition
persistent identity: BBa K206001
display id: BBa_K206001
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD weak
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: Site-directed mutagenesis on <partinfo>I13453</partinfo> with the following primers: Forward: TAATCTTATGGATAAAAAAGCTATGGCATAGC Reverse: GCGGATCCTACCTGACGCTTTTTATC
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weaker version of wild type pBAD (<partinfo>I13453</partinfo>).
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049255
display id: annotation2049255
version: 1
top level: BBa_K206001
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1
type: Range
persistent identity: range2049255
display id: range2049255
version: 1
top level: BBa_K206001
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049256
display id: annotation2049256
version: 1
top level: BBa_K206001
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1
type: Range
persistent identity: range2049256
display id: range2049256
version: 1
top level: BBa_K206001
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049257
display id: annotation2049257
version: 1
top level: BBa_K206001
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1
type: Range
persistent identity: range2049257
display id: range2049257
version: 1
top level: BBa_K206001
direction: direction/forward
start: 61
end: 78
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_K874021
type: ComponentDefinition
persistent identity: BBa K874021
display id: BBa_K874021
version: 1
was generated by: iGEM to SBOL conversion
description: Myc-Linker
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:38Z
mutable provenance: None yet
owned by: james
owned by: myers
mutable description: None yet
discontinued: false
dominant: false
experience: experience/Works
group u list: _1135_
m user id: 0
owner id: 12435
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None yet
star: false
creator: Ernst Bank
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Linker
sequence annotation: Myc
sequence: https://synbiohub.org/public/igem/BBa_K874021_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2191557
display id: annotation2191557
version: 1
top level: BBa_K874021
location: https://synbiohub.org/public/igem/BBa_K874021/annotation2191557/range2191557/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K874021/annotation2191557/range2191557/1
type: Range
persistent identity: range2191557
display id: range2191557
version: 1
top level: BBa_K874021
direction: direction/forward
start: 1
end: 54
orientation: inline

Myc
type: SequenceAnnotation
persistent identity: annotation2191558
display id: annotation2191558
version: 1
top level: BBa_K874021
location: https://synbiohub.org/public/igem/BBa_K874021/annotation2191558/range2191558/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K874021/annotation2191558/range2191558/1
type: Range
persistent identity: range2191558
display id: range2191558
version: 1
top level: BBa_K874021
direction: direction/forward
start: 16
end: 45
orientation: inline

BBa_K874000
type: ComponentDefinition
persistent identity: BBa K874000
display id: BBa_K874000
version: 1
was generated by: iGEM to SBOL conversion
description: M.ScaI Methyltransferase
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:38Z
mutable provenance: Natively found in Streptomyces caespitosus. Sequence obtained from REBASE. Synthesized by IDT.
owned by: james
owned by: myers
mutable description: The M.ScaI protein is a type II methyltransferase (subtype beta) that recognises site on the DNA of the following sequence 5..AGTACT..3. It methylates this site at the 5th (Cytosine) nucleotide leaving ao an N4-methylcytosine (m4). This methylation type (m4) is not found in native E. coli nor is the recognition site methylated by any of E. coli's native methylation systems (Dam, Dcm). This part also includes a flexible linker with incorporated myc-tag at the N terminus of the protein. This will allow for easier creation of fusion proteins and expression verification using westerblot (using the myc antibodies). It can be assumed that M.ScaI (with linker) is expressed and folds properly in E. coli because its function has been verified by the iGEM Amsterdam 2012 team.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1135_
m user id: 0
owner id: 12435
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Contains no forbidden sites (according to the RFC-10 protocol). Contains no sites vulnerable to E. coli's native restriction systems.
star: false
creator: Ernst Bank
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: M.ScaI
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K874000_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2187003
display id: annotation2187003
version: 1
top level: BBa_K874000
location: https://synbiohub.org/public/igem/BBa_K874000/annotation2187003/range2187003/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K874000/annotation2187003/range2187003/1
type: Range
persistent identity: range2187003
display id: range2187003
version: 1
top level: BBa_K874000
direction: direction/forward
start: 1
end: 3
orientation: inline

M.ScaI
type: SequenceAnnotation
persistent identity: annotation2187004
display id: annotation2187004
version: 1
top level: BBa_K874000
location: https://synbiohub.org/public/igem/BBa_K874000/annotation2187004/range2187004/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K874000/annotation2187004/range2187004/1
type: Range
persistent identity: range2187004
display id: range2187004
version: 1
top level: BBa_K874000
direction: direction/forward
start: 1
end: 912
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2187005
display id: annotation2187005
version: 1
top level: BBa_K874000
location: https://synbiohub.org/public/igem/BBa_K874000/annotation2187005/range2187005/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K874000/annotation2187005/range2187005/1
type: Range
persistent identity: range2187005
display id: range2187005
version: 1
top level: BBa_K874000
direction: direction/forward
start: 913
end: 915
orientation: inline

BBa_K874001
type: ComponentDefinition
persistent identity: BBa K874001
display id: BBa_K874001
version: 1
was generated by: iGEM to SBOL conversion
description: Polydactyl Zinc Finger (PZF3838)
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:38Z
mutable provenance: Synthesized by IDT. For more information see individual components descriptions.
owned by: james
owned by: myers
mutable description: Intermediate construct used in the iGEM Amsterdam 2012 project.
discontinued: false
dominant: true
experience: experience/Issues
group u list: _1135_
m user id: 0
owner id: 12435
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: See Individual components
star: false
creator: Ernst Bank
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: Zinc Finger Array
sequence annotation: Zinc Finger 1
sequence annotation: Zinc Finger 2
sequence annotation: Zinc Finger 3
sequence annotation: Zinc Finger 4
sequence annotation: Zinc Finger 5
sequence annotation: Zinc Finger 6
sequence: https://synbiohub.org/public/igem/BBa_K874001_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2192156
display id: annotation2192156
version: 1
top level: BBa_K874001
location: https://synbiohub.org/public/igem/BBa_K874001/annotation2192156/range2192156/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K874001/annotation2192156/range2192156/1
type: Range
persistent identity: range2192156
display id: range2192156
version: 1
top level: BBa_K874001
direction: direction/forward
start: 4
end: 6
orientation: inline

Zinc Finger Array
type: SequenceAnnotation
persistent identity: annotation2192157
display id: annotation2192157
version: 1
top level: BBa_K874001
location: https://synbiohub.org/public/igem/BBa_K874001/annotation2192157/range2192157/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K874001/annotation2192157/range2192157/1
type: Range
persistent identity: range2192157
display id: range2192157
version: 1
top level: BBa_K874001
direction: direction/forward
start: 7
end: 510
orientation: inline

Zinc Finger 1
type: SequenceAnnotation
persistent identity: annotation2200695
display id: annotation2200695
version: 1
top level: BBa_K874001
location: https://synbiohub.org/public/igem/BBa_K874001/annotation2200695/range2200695/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K874001/annotation2200695/range2200695/1
type: Range
persistent identity: range2200695
display id: range2200695
version: 1
top level: BBa_K874001
direction: direction/forward
start: 7
end: 90
orientation: inline

Zinc Finger 2
type: SequenceAnnotation
persistent identity: annotation2200698
display id: annotation2200698
version: 1
top level: BBa_K874001
location: https://synbiohub.org/public/igem/BBa_K874001/annotation2200698/range2200698/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K874001/annotation2200698/range2200698/1
type: Range
persistent identity: range2200698
display id: range2200698
version: 1
top level: BBa_K874001
direction: direction/forward
start: 91
end: 174
orientation: inline

Zinc Finger 3
type: SequenceAnnotation
persistent identity: annotation2200700
display id: annotation2200700
version: 1
top level: BBa_K874001
location: https://synbiohub.org/public/igem/BBa_K874001/annotation2200700/range2200700/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K874001/annotation2200700/range2200700/1
type: Range
persistent identity: range2200700
display id: range2200700
version: 1
top level: BBa_K874001
direction: direction/forward
start: 175
end: 258
orientation: inline

Zinc Finger 4
type: SequenceAnnotation
persistent identity: annotation2200703
display id: annotation2200703
version: 1
top level: BBa_K874001
location: https://synbiohub.org/public/igem/BBa_K874001/annotation2200703/range2200703/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K874001/annotation2200703/range2200703/1
type: Range
persistent identity: range2200703
display id: range2200703
version: 1
top level: BBa_K874001
direction: direction/forward
start: 259
end: 342
orientation: inline

Zinc Finger 5
type: SequenceAnnotation
persistent identity: annotation2200705
display id: annotation2200705
version: 1
top level: BBa_K874001
location: https://synbiohub.org/public/igem/BBa_K874001/annotation2200705/range2200705/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K874001/annotation2200705/range2200705/1
type: Range
persistent identity: range2200705
display id: range2200705
version: 1
top level: BBa_K874001
direction: direction/forward
start: 343
end: 426
orientation: inline

Zinc Finger 6
type: SequenceAnnotation
persistent identity: annotation2200707
display id: annotation2200707
version: 1
top level: BBa_K874001
location: https://synbiohub.org/public/igem/BBa_K874001/annotation2200707/range2200707/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K874001/annotation2200707/range2200707/1
type: Range
persistent identity: range2200707
display id: range2200707
version: 1
top level: BBa_K874001
direction: direction/forward
start: 427
end: 510
orientation: inline

https://synbiohub.org/public/igem/BBa_K874103_sequence/1
type: Sequence
persistent identity: BBa K874103 sequence
display id: BBa_K874103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcttttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagtcacacaggaaagcgcatgggcgaaaagccgtatgcgtgccccgaatgtgggaagtccttttctcgtagcgacgagcttgttcgccaccaaaggacccataccggcgagaagccctacgcctgcccggagtgtggaaagtcgttctcccagagcggcgatttgcgtcggcatcaacggacgcataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgagaaaccatacgcctgtcccgagtgcggtaaaagctttagtcagcgtgcccatcttgaacgccaccagaggacccataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgaaaaaccgtatgcttgtccagaatgtggtaagtccttttctcgtagcgataagctcgttcgccaccaaaggactcataccgccgccagctttcaagaacagaaactcatctctgaagaggatctggcacccggcatgtccgggcgggactttggatatgtgatacagtcgtccgctgcactatggaatcgactctctacattctcacagagaggaaaagccttggacaccaggcttgcagacatcaagaaggccctggggaagccgtactacgaaacctcggatgtccttctttaccacggcgacagtcttgagctgctcaagtcaatgcctcagcagattttcgaccttaccgtaactagcccaccttacaatattggcaaagagtacgagggtgtactgtcgatcgaggaatacatttcctggtgcgagacatggatgtcgcgcgttcatagggcgaccagcgcaggcggcgcattttggctcaatgttgggtacgtccctgtcccgaaccaaggaaaagcagtcccgattccttacctcttgtgggacaagagtccgttctacatgatccaggaagttgtctggaattacggggcgggagtggcgtctcgaaaatcgttttccccgcgcaatgaaaagtttctctggtatgtgcgcgacccgctgaattattacttcgacctcgattcggtgcgcgacccaaatgtgaaataccccaaccagaaaaagaatgggaagctcaaatgcaacccgttggggaaaaatcccactgacgtttggcagttccccaaggttacgtcgggcgcgaagagatcaagcgtggagcgcaccgcccatccggcacaattcccgtctgctgtcattgaacgggtcatcaaggcgtgcagcccttccgacggcgtcatcctggacccattcctcggttccggaacgacctcgctgaccgccagaaagcaaggccggtgcagcgtcggtatcgaaatccgcgaagactacctcgacatcgcggtgggacgcctggaggcggaggcgcaatccctcttctagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05074_sequence/1
type: Sequence
persistent identity: BBa S05074 sequence
display id: BBa_S05074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagcgcatgggcgaaaagccgtatgcgtgccccgaatgtgggaagtccttttctcgtagcgacgagcttgttcgccaccaaaggacccataccggcgagaagccctacgcctgcccggagtgtggaaagtcgttctcccagagcggcgatttgcgtcggcatcaacggacgcataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgagaaaccatacgcctgtcccgagtgcggtaaaagctttagtcagcgtgcccatcttgaacgccaccagaggacccataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgaaaaaccgtatgcttgtccagaatgtggtaagtccttttctcgtagcgataagctcgttcgccaccaaaggactcataccgccgccagctttcaagaacagaaactcatctctgaagaggatctggcacccggcatgtccgggcgggactttggatatgtgatacagtcgtccgctgcactatggaatcgactctctacattctcacagagaggaaaagccttggacaccaggcttgcagacatcaagaaggccctggggaagccgtactacgaaacctcggatgtccttctttaccacggcgacagtcttgagctgctcaagtcaatgcctcagcagattttcgaccttaccgtaactagcccaccttacaatattggcaaagagtacgagggtgtactgtcgatcgaggaatacatttcctggtgcgagacatggatgtcgcgcgttcatagggcgaccagcgcaggcggcgcattttggctcaatgttgggtacgtccctgtcccgaaccaaggaaaagcagtcccgattccttacctcttgtgggacaagagtccgttctacatgatccaggaagttgtctggaattacggggcgggagtggcgtctcgaaaatcgttttccccgcgcaatgaaaagtttctctggtatgtgcgcgacccgctgaattattacttcgacctcgattcggtgcgcgacccaaatgtgaaataccccaaccagaaaaagaatgggaagctcaaatgcaacccgttggggaaaaatcccactgacgtttggcagttccccaaggttacgtcgggcgcgaagagatcaagcgtggagcgcaccgcccatccggcacaattcccgtctgctgtcattgaacgggtcatcaaggcgtgcagcccttccgacggcgtcatcctggacccattcctcggttccggaacgacctcgctgaccgccagaaagcaaggccggtgcagcgtcggtatcgaaatccgcgaagactacctcgacatcgcggtgggacgcctggaggcggaggcgcaatccctcttctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206001_sequence/1
type: Sequence
persistent identity: BBa K206001 sequence
display id: BBa_K206001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcttttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K874021_sequence/1
type: Sequence
persistent identity: BBa K874021 sequence
display id: BBa_K874021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccgccagctttcaagaacagaaactcatctctgaagaggatctggcacccggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K874000_sequence/1
type: Sequence
persistent identity: BBa K874000 sequence
display id: BBa_K874000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccgggcgggactttggatatgtgatacagtcgtccgctgcactatggaatcgactctctacattctcacagagaggaaaagccttggacaccaggcttgcagacatcaagaaggccctggggaagccgtactacgaaacctcggatgtccttctttaccacggcgacagtcttgagctgctcaagtcaatgcctcagcagattttcgaccttaccgtaactagcccaccttacaatattggcaaagagtacgagggtgtactgtcgatcgaggaatacatttcctggtgcgagacatggatgtcgcgcgttcatagggcgaccagcgcaggcggcgcattttggctcaatgttgggtacgtccctgtcccgaaccaaggaaaagcagtcccgattccttacctcttgtgggacaagagtccgttctacatgatccaggaagttgtctggaattacggggcgggagtggcgtctcgaaaatcgttttccccgcgcaatgaaaagtttctctggtatgtgcgcgacccgctgaattattacttcgacctcgattcggtgcgcgacccaaatgtgaaataccccaaccagaaaaagaatgggaagctcaaatgcaacccgttggggaaaaatcccactgacgtttggcagttccccaaggttacgtcgggcgcgaagagatcaagcgtggagcgcaccgcccatccggcacaattcccgtctgctgtcattgaacgggtcatcaaggcgtgcagcccttccgacggcgtcatcctggacccattcctcggttccggaacgacctcgctgaccgccagaaagcaaggccggtgcagcgtcggtatcgaaatccgcgaagactacctcgacatcgcggtgggacgcctggaggcggaggcgcaatccctcttctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K874001_sequence/1
type: Sequence
persistent identity: BBa K874001 sequence
display id: BBa_K874001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcatgggcgaaaagccgtatgcgtgccccgaatgtgggaagtccttttctcgtagcgacgagcttgttcgccaccaaaggacccataccggcgagaagccctacgcctgcccggagtgtggaaagtcgttctcccagagcggcgatttgcgtcggcatcaacggacgcataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgagaaaccatacgcctgtcccgagtgcggtaaaagctttagtcagcgtgcccatcttgaacgccaccagaggacccataccggcgagaagccttacgcttgcccggagtgtggcaagtcattcagcgactgccgtgatctcgcaaggcatcaacgcacccataccggcgaaaaaccgtatgcttgtccagaatgtggtaagtccttttctcgtagcgataagctcgttcgccaccaaaggactcatacc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z