LOCUS       BBa_K874200             2022 bp    DNA     linear       17-May-2024
DEFINITION  IPTG inducible PZF targeted methylation and measurement system 
            (dual PZF3838 targeted)
ACCESSION   BBa_K874200
VERSION     BBa_K874200.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     misc_feature    80..82
                     /label=start
     CDS             83..586
                     /label=Zinc Finger Array
     CDS             83..166
                     /label=Zinc Finger 1
     CDS             167..250
                     /label=Zinc Finger 2
     CDS             251..334
                     /label=Zinc Finger 3
     CDS             335..418
                     /label=Zinc Finger 4
     CDS             419..502
                     /label=Zinc Finger 5
     CDS             503..586
                     /label=Zinc Finger 6
     CDS             77..586
                     /label=BBa_K874001
     CDS             587..640
                     /label=Linker
     misc_feature    602..631
                     /label=Myc
     misc_feature    587..640
                     /label=BBa_K874021
     misc_feature    641..643
                     /label=start
     CDS             641..1552
                     /label=M.ScaI
     misc_feature    1553..1555
                     /label=stop
     CDS             641..1555
                     /label=BBa_K874000
     misc_feature    64..1555
                     /label=BBa_S05074
     stem_loop       1571..1590
                     /label=T7 TE
     misc_feature    1597..1597
                     /label=stop
     polyA_site      1591..1604
                     /label=polya
     misc_feature    1564..1604
                     /label=BBa_B0012
     terminator      1564..1604
                     /label=BBa_B0012
     stem_loop       1625..1647
                     /label=stem_loop
     misc_feature    1613..1658
                     /label=BBa_B0011
     terminator      1613..1658
                     /label=BBa_B0011
     terminator      1564..1658
                     /label=BBa_B0014
     misc_feature    1..1658
                     /label=BBa_K874102
     misc_feature    1669..1673
                     /label=BamHI
     misc_feature    1674..1799
                     /label=125pb Spacer
     misc_feature    1659..1799
                     /label=BBa_K874040
     misc_feature    1800..1816
                     /label=E2C Binding Site
     misc_feature    1874..1890
                     /label=E2C Binding Site (rev. comp.)
     misc_feature    1817..1841
                     /label=25bp Spacer
     misc_feature    1849..1873
                     /label=25bp Spacer
     misc_feature    1842..1848
                     /label=M/R Site
     misc_feature    1800..1891
                     /label=BBa_K874060
     misc_feature    2017..2022
                     /label=NdeI
     misc_feature    1892..2016
                     /label=125bp Spacer
     misc_feature    1892..2022
                     /label=BBa_K874041
     misc_feature    1659..2022
                     /label=BBa_K874120
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagcgca tgggcgaaaa gccgtatgcg tgccccgaat gtgggaagtc
      121 cttttctcgt agcgacgagc ttgttcgcca ccaaaggacc cataccggcg agaagcccta
      181 cgcctgcccg gagtgtggaa agtcgttctc ccagagcggc gatttgcgtc ggcatcaacg
      241 gacgcatacc ggcgagaagc cttacgcttg cccggagtgt ggcaagtcat tcagcgactg
      301 ccgtgatctc gcaaggcatc aacgcaccca taccggcgag aaaccatacg cctgtcccga
      361 gtgcggtaaa agctttagtc agcgtgccca tcttgaacgc caccagagga cccataccgg
      421 cgagaagcct tacgcttgcc cggagtgtgg caagtcattc agcgactgcc gtgatctcgc
      481 aaggcatcaa cgcacccata ccggcgaaaa accgtatgct tgtccagaat gtggtaagtc
      541 cttttctcgt agcgataagc tcgttcgcca ccaaaggact cataccgccg ccagctttca
      601 agaacagaaa ctcatctctg aagaggatct ggcacccggc atgtccgggc gggactttgg
      661 atatgtgata cagtcgtccg ctgcactatg gaatcgactc tctacattct cacagagagg
      721 aaaagccttg gacaccaggc ttgcagacat caagaaggcc ctggggaagc cgtactacga
      781 aacctcggat gtccttcttt accacggcga cagtcttgag ctgctcaagt caatgcctca
      841 gcagattttc gaccttaccg taactagccc accttacaat attggcaaag agtacgaggg
      901 tgtactgtcg atcgaggaat acatttcctg gtgcgagaca tggatgtcgc gcgttcatag
      961 ggcgaccagc gcaggcggcg cattttggct caatgttggg tacgtccctg tcccgaacca
     1021 aggaaaagca gtcccgattc cttacctctt gtgggacaag agtccgttct acatgatcca
     1081 ggaagttgtc tggaattacg gggcgggagt ggcgtctcga aaatcgtttt ccccgcgcaa
     1141 tgaaaagttt ctctggtatg tgcgcgaccc gctgaattat tacttcgacc tcgattcggt
     1201 gcgcgaccca aatgtgaaat accccaacca gaaaaagaat gggaagctca aatgcaaccc
     1261 gttggggaaa aatcccactg acgtttggca gttccccaag gttacgtcgg gcgcgaagag
     1321 atcaagcgtg gagcgcaccg cccatccggc acaattcccg tctgctgtca ttgaacgggt
     1381 catcaaggcg tgcagccctt ccgacggcgt catcctggac ccattcctcg gttccggaac
     1441 gacctcgctg accgccagaa agcaaggccg gtgcagcgtc ggtatcgaaa tccgcgaaga
     1501 ctacctcgac atcgcggtgg gacgcctgga ggcggaggcg caatccctct tctagtacta
     1561 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata
     1621 taaaaagcca gattattaat ccggcttttt tattatttcg cccggcgcgg atccctcgaa
     1681 gtatcgtcat ttacacgttg tcggtcccag acgtgactac ttcgaggtcc cccgagcttt
     1741 cgggtccgat ttacttgccc tgagagtgat tgggaaacga tacgcgcgta caactggtgg
     1801 gggccggagc cgcagtgtcc tattgtccgt ggtaccggtg atagtactac tagaatagga
     1861 ggggctatat ccacactgcg gctccggccc ctccacctag ttgtcaaggg gttgtagagt
     1921 gcggtctttc acacatgtcg ccacctacaa ggctttcaga accagaactt gtagtgtgac
     1981 ttttttcggc ggtggagtcg ccgcagcaat ggaccgcata tg
//