##gff-version 3
##sequence-region BBa_K874300 1 1154
BBa_K874300	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K874300	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K874300	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K874300	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K874300	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K874300	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K874300	.	engineered_region	64	1154	.	+	0	ID=BBa_S05078;Name=BBa_S05078
BBa_K874300	.	sequence_feature	1052	1059	.	+	0	ID=BBa_K874090;Name=BBa_K874090;Parent=BBa_S05078
BBa_K874300	.	engineered_region	64	1051	.	+	0	ID=BBa_S05066;Name=BBa_S05066;Parent=BBa_S05078
BBa_K874300	.	CDS	137	1051	.	+	0	ID=BBa_K874000;Name=BBa_K874000;Parent=BBa_S05066
BBa_K874300	.	stop_codon	1049	1051	.	+	0	ID=annotation2187005;Name=stop;Parent=BBa_K874000
BBa_K874300	.	start_codon	137	139	.	+	0	ID=annotation2187003;Name=start;Parent=BBa_K874000
BBa_K874300	.	CDS	137	1048	.	+	0	ID=annotation2187004;Name=M.ScaI;Parent=BBa_K874000
BBa_K874300	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05066
BBa_K874300	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K874300	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K874300	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K874300	.	terminator	1060	1154	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_S05078
BBa_K874300	.	terminator	1060	1100	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K874300	.	polyA_site	1087	1100	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K874300	.	stop_codon	1093	1093	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K874300	.	engineered_region	1060	1100	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K874300	.	stem_loop	1067	1086	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K874300	.	terminator	1109	1154	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K874300	.	stem_loop	1121	1143	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K874300	.	engineered_region	1109	1154	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
>BBa_K874300
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagcgc
atggccgccagctttcaagaacagaaactcatctctgaagaggatctggcacccggcatgtccgggcgggactttggat
atgtgatacagtcgtccgctgcactatggaatcgactctctacattctcacagagaggaaaagccttggacaccaggct
tgcagacatcaagaaggccctggggaagccgtactacgaaacctcggatgtccttctttaccacggcgacagtcttgag
ctgctcaagtcaatgcctcagcagattttcgaccttaccgtaactagcccaccttacaatattggcaaagagtacgagg
gtgtactgtcgatcgaggaatacatttcctggtgcgagacatggatgtcgcgcgttcatagggcgaccagcgcaggcgg
cgcattttggctcaatgttgggtacgtccctgtcccgaaccaaggaaaagcagtcccgattccttacctcttgtgggac
aagagtccgttctacatgatccaggaagttgtctggaattacggggcgggagtggcgtctcgaaaatcgttttccccgc
gcaatgaaaagtttctctggtatgtgcgcgacccgctgaattattacttcgacctcgattcggtgcgcgacccaaatgt
gaaataccccaaccagaaaaagaatgggaagctcaaatgcaacccgttggggaaaaatcccactgacgtttggcagttc
cccaaggttacgtcgggcgcgaagagatcaagcgtggagcgcaccgcccatccggcacaattcccgtctgctgtcattg
aacgggtcatcaaggcgtgcagcccttccgacggcgtcatcctggacccattcctcggttccggaacgacctcgctgac
cgccagaaagcaaggccggtgcagcgtcggtatcgaaatccgcgaagactacctcgacatcgcggtgggacgcctggag
gcggaggcgcaatccctcttctagcactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agagagaatataaaaagccagattattaatccggcttttttattattt