BBa_K875003

BBa_K875003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K875003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Federico Colombo, Elisa Clagnan

Date created: 2012-09-22 11:00:00

Date modified: 2015-05-08 01:13:38

CymR

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K875003_sequence (Version 1)

Description

This composite produces constitutively the CymR regulator protein, that binds the Cumate Operator repressing the transcription from the promoter.

Notes

The CymR has a SV5 tag at the C-terminus in order to be detected by an anti-SV5 Ab.

Source

1. The cumate gene-switch: a system for regulated expression in mammalian cells
Alaka Mullick, Yan Xu, Ren?? Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, F??lix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie ( BMC Biotechnology 2006, 6:43 doi:10.1186/1472-6750-6-43 )
2. Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains
Young J. Choi, Lyne Morel, Teffanie Le Fran??ois Denis Bourque, Lucie Bourget, Denis Groleau, Bernard Massie and Carlos B. M??guez (Applied and Environmental Microbiology, Aug. 2010, p. 5058???5066 Vol.76 doi:10.1128/AEM.00413-10)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23100	1,35	feature/promoter, promoter
CymR	62,679	feature/cds, CDS
BBa_B0034	44,55	ribosome_entry_site, feature/rbs
SV5 Tag	680,724	tag, feature/tag
BBa_B0015	739,867	feature/stem_loop, stem_loop

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K875003/1

Member of these Collections

• iGEM Parts Registry